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Test no. 487: in vitro mammalian cell micronucleus test

Author: Organisation for Economic Co-operation and Development.
Publisher: Paris : OECD Publishing, 2016.
Series: OECD Guidelines for the Testing of Chemicals., Section 4.
Edition/Format:   eBook : Document : International government publication : EnglishView all editions and formats
Summary:
The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that are unable to migrate to the poles during the anaphase stage of cell division. The assay detects the activity of clastogenic and aneugenic test substances in cells that have  Read more...
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Details

Material Type: Document, Government publication, International government publication, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Organisation for Economic Co-operation and Development.
ISBN: 9789264264861 9264264868
OCLC Number: 961480360
Description: 1 online resource (29 pages).
Series Title: OECD Guidelines for the Testing of Chemicals., Section 4.
Other Titles: In vitro mammalian cell micronucleus test
TG 487
Responsibility: Organisation for Economic Co-operation and Development.

Abstract:

The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that are unable to migrate to the poles during the anaphase stage of cell division. The assay detects the activity of clastogenic and aneugenic test substances in cells that have undergone cell division during or after exposure to the test substance. This Test Guideline allows the use of protocols with and without the actin polymerisation inhibitor cytochalasin B. Cytochalasin B allows for the identification and selective analysis of micronucleus frequency in cells that have completed one mitosis, because such cells are binucleate. This Test Guideline also allows the use of protocols without cytokinesis block provided there is evidence that the cell population analysed has undergone mitosis.

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