skip to content
Test No. 490: In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene Preview this item
ClosePreview this item
Checking...

Test No. 490: In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene

Author: Organisation for Economic Co-operation and Development.
Publisher: Paris : OECD Publishing, 2015.
Series: OECD Guidelines for the Testing of Chemicals, Section 4
Edition/Format:   eBook : Document : International government publication : EnglishView all editions and formats
Summary:
The in vitro mammalian cell gene mutation test can be used to detect gene mutations induced by chemical substances. This TG includes two distinct in vitro mammalian gene mutation assays requiring two specific tk heterozygous cells lines: L5178Y tk+/-3.7.2C cells for the mouse lymphoma assay (MLA) and TK6 tk+/- cells for the TK6 assay. Genetic events detected using the tk locus include both gene mutations and  Read more...
Rating:

(not yet rated) 0 with reviews - Be the first.

Subjects
More like this

 

Find a copy online

Links to this item

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Material Type: Document, Government publication, International government publication, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Organisation for Economic Co-operation and Development.
ISBN: 9789264242241 9264242244
OCLC Number: 927186747
Description: 1 online resource (25 pages).
Series Title: OECD Guidelines for the Testing of Chemicals, Section 4
Responsibility: Organisation for Economic Co-operation and Development.

Abstract:

The in vitro mammalian cell gene mutation test can be used to detect gene mutations induced by chemical substances. This TG includes two distinct in vitro mammalian gene mutation assays requiring two specific tk heterozygous cells lines: L5178Y tk+/-3.7.2C cells for the mouse lymphoma assay (MLA) and TK6 tk+/- cells for the TK6 assay. Genetic events detected using the tk locus include both gene mutations and chromosomal events. Cells in suspension or monolayer culture are exposed to, at least four analysable concentrations of the test substance, both with and without metabolic activation, for a suitable period of time. They are subcultured to determine cytotoxicity and to allow phenotypic expression prior to mutant selection. Cytotoxicity is usually determined by measuring the relative cloning efficiency (survival) or relative total growth of the cultures after the treatment period. The treated cultures are maintained in growth medium for a sufficient period of time, characteristic of each selected locus and cell type, to allow near-optimal phenotypic expression of induced mutations. Mutant frequency is determined by seeding known numbers of cells in medium containing the selective agent to detect mutant cells, and in medium without selective agent to determine the cloning efficiency (viability). After a suitable incubation time, colonies are counted.

Reviews

User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.

Similar Items

Related Subjects:(2)

Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Linked Data


Primary Entity

<http://www.worldcat.org/oclc/927186747> # Test No. 490: In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene
    a schema:MediaObject, schema:Book, schema:CreativeWork ;
   library:oclcnum "927186747" ;
   library:placeOfPublication <http://experiment.worldcat.org/entity/work/data/2789537284#Place/paris> ; # Paris
   library:placeOfPublication <http://id.loc.gov/vocabulary/countries/fr> ;
   rdfs:comment "Unknown 'gen' value: igp" ;
   schema:about <http://experiment.worldcat.org/entity/work/data/2789537284#Topic/environment> ; # Environment
   schema:about <http://experiment.worldcat.org/entity/work/data/2789537284#Topic/adaptation_biology> ; # Adaptation (Biology)
   schema:bookFormat schema:EBook ;
   schema:creator <http://experiment.worldcat.org/entity/work/data/2789537284#Organization/organisation_for_economic_co_operation_and_development> ; # Organisation for Economic Co-operation and Development.
   schema:datePublished "2015" ;
   schema:description "The in vitro mammalian cell gene mutation test can be used to detect gene mutations induced by chemical substances. This TG includes two distinct in vitro mammalian gene mutation assays requiring two specific tk heterozygous cells lines: L5178Y tk+/-3.7.2C cells for the mouse lymphoma assay (MLA) and TK6 tk+/- cells for the TK6 assay. Genetic events detected using the tk locus include both gene mutations and chromosomal events. Cells in suspension or monolayer culture are exposed to, at least four analysable concentrations of the test substance, both with and without metabolic activation, for a suitable period of time. They are subcultured to determine cytotoxicity and to allow phenotypic expression prior to mutant selection. Cytotoxicity is usually determined by measuring the relative cloning efficiency (survival) or relative total growth of the cultures after the treatment period. The treated cultures are maintained in growth medium for a sufficient period of time, characteristic of each selected locus and cell type, to allow near-optimal phenotypic expression of induced mutations. Mutant frequency is determined by seeding known numbers of cells in medium containing the selective agent to detect mutant cells, and in medium without selective agent to determine the cloning efficiency (viability). After a suitable incubation time, colonies are counted."@en ;
   schema:exampleOfWork <http://worldcat.org/entity/work/id/2789537284> ;
   schema:genre "Government publication"@en ;
   schema:inLanguage "en" ;
   schema:isPartOf <http://worldcat.org/issn/2074-5788> ; # OECD Guidelines for the Testing of Chemicals, Section 4,
   schema:name "Test No. 490: In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene"@en ;
   schema:productID "927186747" ;
   schema:publication <http://www.worldcat.org/title/-/oclc/927186747#PublicationEvent/paris_oecd_publishing_2015> ;
   schema:publisher <http://experiment.worldcat.org/entity/work/data/2789537284#Agent/oecd_publishing> ; # OECD Publishing
   schema:url <http://ezproxy.lib.ryerson.ca/login?url=http://dx.doi.org/10.1787/9789264242241-en> ;
   schema:url <http://dx.doi.org/10.1787/9789264242241-en> ;
   schema:workExample <http://worldcat.org/isbn/9789264242241> ;
   schema:workExample <http://dx.doi.org/10.1787/9789264242241-en> ;
   wdrs:describedby <http://www.worldcat.org/title/-/oclc/927186747> ;
    .


Related Entities

<http://dx.doi.org/10.1787/9789264242241-en>
    a schema:IndividualProduct ;
   rdfs:comment "e-book" ;
   rdfs:comment "eBook available for UOIT via OECD iLibrary. Click link to access" ;
    .

<http://experiment.worldcat.org/entity/work/data/2789537284#Agent/oecd_publishing> # OECD Publishing
    a bgn:Agent ;
   schema:name "OECD Publishing" ;
    .

<http://experiment.worldcat.org/entity/work/data/2789537284#Organization/organisation_for_economic_co_operation_and_development> # Organisation for Economic Co-operation and Development.
    a schema:Organization ;
   schema:name "Organisation for Economic Co-operation and Development." ;
    .

<http://experiment.worldcat.org/entity/work/data/2789537284#Topic/adaptation_biology> # Adaptation (Biology)
    a schema:Intangible ;
   schema:name "Adaptation (Biology)"@en ;
    .

<http://worldcat.org/isbn/9789264242241>
    a schema:ProductModel ;
   schema:isbn "9264242244" ;
   schema:isbn "9789264242241" ;
    .

<http://worldcat.org/issn/2074-5788> # OECD Guidelines for the Testing of Chemicals, Section 4,
    a bgn:PublicationSeries ;
   schema:hasPart <http://www.worldcat.org/oclc/927186747> ; # Test No. 490: In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene
   schema:issn "2074-5788" ;
   schema:name "OECD Guidelines for the Testing of Chemicals, Section 4," ;
    .

<http://www.worldcat.org/title/-/oclc/927186747>
    a genont:InformationResource, genont:ContentTypeGenericResource ;
   schema:about <http://www.worldcat.org/oclc/927186747> ; # Test No. 490: In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene
   schema:dateModified "2017-12-22" ;
   void:inDataset <http://purl.oclc.org/dataset/WorldCat> ;
    .


Content-negotiable representations

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.