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Therapeutic oligonucleotides : methods and protocols

Author: John Goodchild
Publisher: New York : Humana Press : Springer, ©2011.
Series: Methods in molecular biology (Clifton, N.J.), v. 764.; Springer protocols (Series)
Edition/Format:   Print book : EnglishView all editions and formats
Summary:
[Publisher-supplied data] The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed  Read more...
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Genre/Form: Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: John Goodchild
ISBN: 9781617791871 1617791873 9781617791888 1617791881
OCLC Number: 728580587
Description: xi, 340 pages : illustrations ; 27 cm.
Contents: Therapeutic oligonucleotides / John Goodchild --
Dinucleotides containing 3-S-phosphorothiolate linkages / James W. Gaynor and Richard Cosstick --
2-O,4-C-methyleneoxymethylene bridged nucleic acids (2,4-BNACOC) / Yoshiyuki Hari [and others] --
Non-covalent peptide-based strategy for ex vivo and in vivo oligonucleotide delivery / Laurence Crombez [and others] --
Cell-penetrating peptides-based strategies for the delivery of splice redirecting antisense oligonucleotides / Samir El Andaloussi [and others] --
Nanoparticle for tumor targeted delivery of oligomers / Xinrong Liu, Yi Wang, and Donald J. Hnatowich --
Light-directed delivery of nucleic acids / Sigurd Bøe [and others] --
Antibody targeted siRNA delivery / Masoud M. Toloue and Lance P. Ford --
Aptamer-drug conjugation for targeted tumor cell therapy / Michael J. Donovan [and others] --
Five-step process for screening antisense compounds for efficacy: gene target IL-12Rb2 / Nikki B. Marshall, Laura L. Hauck, and Dan V. Mourich --
Diverse small non-coding RNAs in RNA interference pathways / Liande Li and Yi Liu --
Quantification of siRNAs in vitro and in vivo / Angie Cheng, Alexander V. Vlassov, and Susan Magdaleno --
Optimization of transfection conditions and analysis of siRNA potency using real-time PCR / Angie Cheng, Susan Magdaleno, and Alexander V. Vlassov --
siRNA knockdown of gene expression in endothelial cells / Emily Dennstedt and Brad Bryan --
Using RNA interference in Schistosoma mansoni / Rita Bhardwaj, Greice Krautz-Peterson, and Patrick J. Skelly --
Performing the labeled microRNA pull-down (LAMP) assay system : an experimental approach for high-throughput identification of microRNA-target mRNAs / Ren-Jun Hsu and Huai-Jen Tsai --
Synthesis, purification, and characterization of oligoribonucleotides that act as agonists of TLR7 and/or TLR8 / Tao Lan and Ekambar R. Kandimalla --
Synthesis, purification, and characterization of immune-modulatory oligodeoxynucleotides that act as agonists of toll-like receptor 9 / Mallikarjuna Reddy Putta, Dong Yu, and Ekambar R. Kandimalla --
Surface plasmon resonance investigation of RNA aptamer-RNA ligand interactions / Carmelo Di Primo, Eric Dausse, and Jean-Jacques Toulme --
Practical considerations for analyzing antigene RNAs (agRNAs) : RNA immunoprecipitation of argonaute protein / Jacob C. Schwartz and David R. Corey --
Inhibition of human papillomavirus expression using DNAzymes / Maria Luisa Benitez-Hess, Pablo Reyes-Gutierrez, and Luis Marat Alvarez-Salas.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 764.; Springer protocols (Series)
Responsibility: edited by John Goodchild.
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Abstract:

This volume in the Methods in Molecular Biology (TM) series presents established and emerging methods for the application of oligonucleotides as therapeutics. Includes protocols for optimizing cell  Read more...

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From the reviews:"The book is written for both professionals and novices interested in the field of oligonucleotide therapeutics. It also would be appropriate for graduate students and research Read more...

 
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