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Tools and techniques in biomolecular science

Author: Aysha Divan; Janice Royds
Publisher: Oxford : Oxford University Press, 2013.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

Tools and Techniques in the Biomolecular Sciences reviews a broad range of modern technologies, explaining the theoretical principles of each technology, their applications and limitations, and how  Read more...

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Document Type: Book
All Authors / Contributors: Aysha Divan; Janice Royds
ISBN: 9780199695560 0199695563
OCLC Number: 851397787
Notes: Includes index.
Formerly CIP.
Description: xv, 519 pages : illustrations ; 27 cm
Contents: Machine generated contents note: pt. 1 Working with DNA and RNA --
1. Gene cloning essentials --
1.1. Introduction --
1.2. Gene cloning applications --
1.3. Gene cloning in the laboratory --
1.4. Gene cloning processes --
1.5. Further types of gene cloning --
1.6. Chapter summary --
2. Polymerase chain reaction --
2.1. Introduction --
2.2. How PCR works --
2.3. The PCR protocol --
2.4. PCR techniques and applications --
2.5. Forensic DNA analysis --
2.6. Future prospects --
2.7. Chapter summary --
3. DNA mutagenesis --
3.1. Introduction --
3.2. Rational or site-directed mutagenesis --
3.3. Uracil-containing DNA --
3.4. QuikChange® mutagenesis --
3.5. Multi-site mutagenesis --
3.6. Cassette mutagenesis --
3.7. PCR mutagenesis --
3.8. Saturation mutagenesis --
3.9. Random mutagenesis --
3.10. Chapter summary --
4. DNA sequencing --
4.1. First-generation sequencing --
4.2. Next-generation sequencing --
4.3. Third-generation sequencing --
4.4. Chapter summary --
5. Measuring DNA-protein interactions. Contents note continued: 5.1. Introduction --
5.2. Footprinting --
5.3. Electrophoretic mobility shift assay (EMSA) --
5.4. Chromatin immunoprecipitation --
5.5. Chapter summary --
6. RNA interference technology --
6.1. Regulation of gene expression: the RNA interference pathway --
6.2. Applications of RNAi --
6.3. Short interfering RNA (siRNA) --
6.4. Short hairpin RNA (shRNA) --
6.5. Targeted gene silencing: therapeutic possibilities --
6.6. Chapter summary --
pt. 2 Working with protein --
7. Recombinant protein expression --
7.1. Introduction --
7.2. Choosing the right expression system --
7.3.E. coli-based expression systems --
7.4. Yeast expression systems --
7.5. Baculovirus-insect larval cell expression system --
7.6. Chapter summary --
8. Protein purification --
8.1. Introduction --
8.2. Devising a purification strategy --
8.3. Cell lysis and disruption --
8.4. Performing chromatography --
8.5. Chromatographic separation methods --
8.6. Measuring protein recovery --
8.7. Chapter summary. Contents note continued: 9. Antibodies as research tools --
9.1. Introduction --
9.2. Antibody structure and function --
9.3. Target detection and visualization --
9.4. Do I need to make a new antibody? --
9.5.Common experimental platforms for immunoassay --
9.6. Chapter summary --
10. Measuring protein-protein interactions: qualitative approaches --
10.1. Introduction --
10.2. Pull-down methods --
10.3. Phage display for analysis of protein interactions --
10.4. Yeast two-hybrid assay --
10.5. Chapter summary --
11. Measuring protein-protein interactions: quantitative approaches --
11.1. Introduction --
11.2. Fluorescence spectroscopy --
11.3. Isothermal titration microcalorimetry --
11.4. Surface plasmon resonance --
11.5. Chapter summary --
12. Structural analysis of proteins: X-ray crystallography, NMR, AFM, and CD spectroscopy --
12.1.X-ray crystallography --
12.2. Nuclear magnetic resonance --
12.3. Atomic force microscopy --
12.4. Circular dichroism spectroscopy --
12.5. Chapter summary. Contents note continued: 13. Mass spectrometry --
13.1. Introduction --
13.2. Sample preparation and ionization --
13.3. Mass analysis --
13.4. Tandem mass spectrometry --
13.5. Chapter summary --
14. Proteomic analysis --
14.1. Introduction --
14.2. Gel-based proteomics --
14.3. Protein identification by tandem mass spectrometry (MS/MS) --
14.4. Post-translational modifications --
14.5. Quantitative mass spectrometry --
14.6. Data analysis --
14.7. Chapter summary --
pt. 3 Working with cells and tissues --
15. Culturing mammalian cells --
15.1. Introduction --
15.2. Culturing cells in vitro: essential principles --
15.3. General applications --
15.4. Human embryonic and adult stem cells: culture, isolation, and expansion --
15.5. Stem cell culture protocols --
15.6. Limitations of cell culturing methods --
15.7. Ethical and storage issues --
15.8. Chapter summary --
16. Flow cytometry --
16.1. Flow cytometers: how they work --
16.2. Data display and analysis. Contents note continued: 16.3. Flow cytometry application: immunophenotyping --
16.4. Flow cytometry application: cell cycle analysis --
16.5. Flow cytometry application: apoptosis and viability assessment --
16.6. Cell sorting --
16.7. Chapter summary --
17. Bioimaging: light and electron microscopy --
17.1. Fluorescence microscopy --
17.2. Transmission electron microscopy --
17.3. Correlative light electron microscopy --
17.4. Chapter summary --
18. Histopathology in biomolecular research --
18.1. Introduction --
18.2. Ethical approval and consent --
18.3. Tissue organization and data storage --
18.4. Obtaining tissue samples for research --
18.5. Recognizing pathology --
18.6. Tissue preparation --
18.7. Microscopic analysis of tissue samples --
18.8. Chapter summary --
pt. 4 Working with models in the biomolecular sciences --
19. Mouse models in bioscience research --
19.1. Introduction --
19.2. Transgenic mice --
19.3. Gene targeted mice. Contents note continued: 19.4. Regulatable gene expression systems (Cre-lox models) --
19.5. Regulatable gene expression systems (Tet-Off/Tet-On mice) --
19.6. Chapter summary --
20. Mathematical models in biomolecular sciences --
20.1. Introduction --
20.2. Difference equations --
20.3. Cellular automata --
20.4.Networks --
20.5. Markov chains --
20.6. Petri nets --
20.7. Ordinary differential equations (ODEs) --
20.8. Chapter summary.
Responsibility: edited by Aysha Divan, Janice Royds.

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The book provides a valuable laboratory resource, especially for students trying to understand the theory and background of their experiments. * Professor Bruce Baguley and Dr Euphemia Leung, Read more...

 
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   schema:description "Machine generated contents note: pt. 1 Working with DNA and RNA -- 1. Gene cloning essentials -- 1.1. Introduction -- 1.2. Gene cloning applications -- 1.3. Gene cloning in the laboratory -- 1.4. Gene cloning processes -- 1.5. Further types of gene cloning -- 1.6. Chapter summary -- 2. Polymerase chain reaction -- 2.1. Introduction -- 2.2. How PCR works -- 2.3. The PCR protocol -- 2.4. PCR techniques and applications -- 2.5. Forensic DNA analysis -- 2.6. Future prospects -- 2.7. Chapter summary -- 3. DNA mutagenesis -- 3.1. Introduction -- 3.2. Rational or site-directed mutagenesis -- 3.3. Uracil-containing DNA -- 3.4. QuikChange® mutagenesis -- 3.5. Multi-site mutagenesis -- 3.6. Cassette mutagenesis -- 3.7. PCR mutagenesis -- 3.8. Saturation mutagenesis -- 3.9. Random mutagenesis -- 3.10. Chapter summary -- 4. DNA sequencing -- 4.1. First-generation sequencing -- 4.2. Next-generation sequencing -- 4.3. Third-generation sequencing -- 4.4. Chapter summary -- 5. Measuring DNA-protein interactions."@en ;
   schema:description "Contents note continued: 9. Antibodies as research tools -- 9.1. Introduction -- 9.2. Antibody structure and function -- 9.3. Target detection and visualization -- 9.4. Do I need to make a new antibody? -- 9.5.Common experimental platforms for immunoassay -- 9.6. Chapter summary -- 10. Measuring protein-protein interactions: qualitative approaches -- 10.1. Introduction -- 10.2. Pull-down methods -- 10.3. Phage display for analysis of protein interactions -- 10.4. Yeast two-hybrid assay -- 10.5. Chapter summary -- 11. Measuring protein-protein interactions: quantitative approaches -- 11.1. Introduction -- 11.2. Fluorescence spectroscopy -- 11.3. Isothermal titration microcalorimetry -- 11.4. Surface plasmon resonance -- 11.5. Chapter summary -- 12. Structural analysis of proteins: X-ray crystallography, NMR, AFM, and CD spectroscopy -- 12.1.X-ray crystallography -- 12.2. Nuclear magnetic resonance -- 12.3. Atomic force microscopy -- 12.4. Circular dichroism spectroscopy -- 12.5. Chapter summary."@en ;
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   schema:description "Contents note continued: 5.1. Introduction -- 5.2. Footprinting -- 5.3. Electrophoretic mobility shift assay (EMSA) -- 5.4. Chromatin immunoprecipitation -- 5.5. Chapter summary -- 6. RNA interference technology -- 6.1. Regulation of gene expression: the RNA interference pathway -- 6.2. Applications of RNAi -- 6.3. Short interfering RNA (siRNA) -- 6.4. Short hairpin RNA (shRNA) -- 6.5. Targeted gene silencing: therapeutic possibilities -- 6.6. Chapter summary -- pt. 2 Working with protein -- 7. Recombinant protein expression -- 7.1. Introduction -- 7.2. Choosing the right expression system -- 7.3.E. coli-based expression systems -- 7.4. Yeast expression systems -- 7.5. Baculovirus-insect larval cell expression system -- 7.6. Chapter summary -- 8. Protein purification -- 8.1. Introduction -- 8.2. Devising a purification strategy -- 8.3. Cell lysis and disruption -- 8.4. Performing chromatography -- 8.5. Chromatographic separation methods -- 8.6. Measuring protein recovery -- 8.7. Chapter summary."@en ;
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