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Transcriptional regulation : methods and protocols

Author: Ales Vancura
Publisher: New York, NY : Springer Science+Business Media, LLC, ©2012.
Series: Methods in molecular biology (Clifton, N.J.), 809.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

This book exemplifies recent advances in transcriptional regulation with a set of key cutting-edge protocols from around the world. It explores topics ranging from transcription factors to  Read more...

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Genre/Form: Electronic books
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Ales Vancura
ISBN: 9781617793769 1617793760
OCLC Number: 765960096
Notes: "E-ISSN 1940-6029."
Description: 1 online resource (xvi, 627 pages) : illustrations.
Contents: Genome-wide in vivo cross-linking of sequence-specific transcription factors / Xiao-Yong li and Mark D. Biggin --
Characterization of complex regulatory networks and identification of promoter regulatory elements in yeast : "in silico" And "wet-lab" approaches / Nuno P. Mira, Miguel C. Teixeira, and Isabel Sá-Correia --
Electrophoretic mobility shift assay analysis of NF[kappa]B transcriptional regulation by nuclear I[kappa]B[alpha] / Ashish Juvekar [and others] --
Probing endogenous RNA polymerase II pre-initiation complexes by electrophoretic mobility shift assay / Emmanuelle Wilhelm, Christopher Takacs, and Brendan Bell --
Elucidating protein : DNA complex by oligonucleotide DNA affinity purification / Teddy T.C. Yang and Chi-Wing Chow --
Chromatin immunoprecipitation assay as a tool for analyzing transcription factor activity / Padmaja Gade and Dhan V. Kalvakolanu --
Two-step cross-linking for analysis of protein-chromatin interactions / Bing Tian, Jun Yan, and Allan R. Brasier --
Chromatin immunoprecipitation analysis of NF[kappa]B transcriptional regulation by nuclear I[kappa]B[alpha] in human macrophages / Sitharam Ramaswami [and others] --
In vivo ChIP for the analysis of microdissected tissue samples / Chris Murgatroyd, Anke Hoffmann, and Dietmar Spengler --
Quantification of protein-DNA interactions by in vivo chromatin immunoprecipitation in yeast / Amparo Pascual-Ahuir and Markus Proft --
Mapping protein-DNA interactions using ChIP-sequencing / Charles E. Massie and Ian G. Mils --
ChIP and re-ChIP assays : investigating interactions between regulatory proteins, histone modifications, and the DNA sequences to which they bind / Agnieszka D. Truax and Susanna F. Greer --
Transcriptional regulation of genes via hypoxia-inducible factor / Olga Roche and Michael Ohh --
Exchange protein directly activated by cyclic AMP-1-regulated recruitment of CCAAT/enhancer-binding proteins to the suppressor of cytokine signaling-3 promoter / William A. Sands [and others] --
Computational analysis of promoter elements and chromatin features in yeast / John J. Wyrick --
Chromatin affinity purification / Ryoko Harada and Alain Nepveu --
Determination of histone acetylation status by chromatin immunoprecipitation / Luciano Galdieri, John Moon, and Ales Vancura --
Immunostaining of Drosophila polytene chromosomes to investigate recruitment of chromatin-binding proteins / Magdalena Murawska and Alexander Brehm --
Detection of transcriptional activators, co-activators, and chromatin remodeling by chromatin immunoprecipitation coupled with real-time PCR / Tamara Y. Erkina and Alexandre M. Erkine --
Chromatin endogenous cleavage and psoralen crosslinking assays to analyze rRNA gene chromatin in vivo / Joachim Griesenbeck [and others] --
UV-induced DNA damage and DNA repair in ribosomal genes chromatin / Julie Pelloux [and others] --
Analysis of SUC2 promoter structure by nucleosome scanning / Jennifer Chang and Ales Vancura --
Chromatin immunoprecipitation of mouse embryos / Anne K. Voss [and others] --
Chromatin immunoprecipitation in mouse hippocampal cells and tissues / Badi Sri Sailaja, Takumi Takizawa, and Eran Meshorer --
Approaches for studying nucleosome movement by ATP-dependent chromatin remodeling complexes / Swetansu K. Hota and Blaine Bartholomew --
Mapping protein-DNA and protein-protein interactions of ATP-dependent chromatin remodelers / Swetansu K. Hota [and others] --
Evaluation of histone-modifying enzymes in stem cell populations / Leanne Stalker and Christopher Wynder --
Purification of multiprotein histone acetyltransferase complexes / Yuan-Liang Wang, Francesco Faiola, and Ernest Martinez --
Reconstitution of active and stoichiometric multisubunit lysine acetyltransferase complexes in insect cells / Kezhi Yan [and others] --
Affinity purification of MLL3/MLL4 histone H3K4 methyltransferase complex / Young-Wook Cho, SunHwa Hong, and Kai Ge --
Methods for analyzing histone citrullination in chromatin structure and gene regulation / Pingxin Li, Jing Hu, and Yanming Wang --
Analysis of mRNA abundance and stability by ribonuclease protection assay / Cristina Romero-López [and others] --
Array-based nuclear run-on analysis / Jinshui Fan [and others] --
In vivo run-on assays to monitor nascent precursor RNA transcripts / Piergiorgio Percipalle and Emilie Louvet --
Genome wide full-length transcript analysis using 5' and 3' paired-end-tag next generation sequencing (RNA-PET) / Xiaoan Ruan and Yijun Ruan --
Analysis of co-transcriptional RNA processing by RNA- ChIP assay / Danielle Bittencourt and Didier Auboeuf --
Quantitative analysis of transcription elongation by RNA polymerase I in vitro / David Alan Schneider --
Detection and characterization of transcription termination / Ghada Ghazal, Jules Gagnon, and Sherif Abou Elela --
Promoter-associated noncoding RNA from the CCND1 promoter / Xiaoyuan Song [and others].
Series Title: Methods in molecular biology (Clifton, N.J.), 809.
Responsibility: edited by Ales Vancura.

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