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Transgenesis techniques : principles and protocols

Author: Alan R Clarke
Publisher: Totowa, NJ : Humana Press, ©2002.
Series: Methods in molecular biology (Clifton, N.J.), v. 180.
Edition/Format:   Print book : English : 2nd edView all editions and formats
Summary:

A collection of reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of mammalian genome. It focuses on the creation and maintenance of  Read more...

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Genre/Form: Laboratory manuals
Manuels de laboratoire
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Alan R Clarke
ISBN: 0896036960 9780896036963
OCLC Number: 46640797
Description: x, 351 pages : illustrations ; 24 cm.
Contents: pt. 1. Topical reviews in transgenesis --
Biomedical and agricultural applications of animal transgenesis / Jim McWhir --
pt. 2. Transgenesis in invertebrate and lower vertebrate species --
Gene transfer in Drosophila / Mark J. O'Connor --
pt. 3. Transgenesis in the mouse: oocyte injection --
Oocyte injection in the mouse / Gary A.J. Brown --
pt. 4. Alternative routes to the germline --
Adenoviral infection / Tohru Tsukui --
Retroviral infection / Richard A. Bowen --
pt. 4. Transgenesis in the mouse: the ES cell route --
In vitro isolation of murine embryonic stem cells / David Wells --
Production of chimeras derived from murine embryonic stem cells / David Wells --
Gene targeting strategies / David W. Melton --
Cre/loxP recombination system and gene targeting / Ralf Kühn --
pt. 6. Cryopreservation of mouse lines --
Cryopreservation of transgenic mouse lines / Jillian M. Shaw --
Ovarian tissue transplantation and cryopreservation: application to maintenance and recovery of transgenic and inbred mouse lines / Jillian M. Shaw --
pt. 7. Transgenesis in the rat --
Transgenesis in the rat / Linda J. Mullins --
pt. 8. Transgenesis in domestic species --
Generation of transgenic livestock by pronuclear injection / A. John Clark --
Transgenic sheep from cultured cells / Keith H.S. Campbell --
pt. 9. Characterization and analysis of transgenic strains --
Analysis of transgenic mice / Stefan Selbert.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 180.
Responsibility: edited by Alan R. Clarke.
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From Reviews of the First Edition a must in any laboratory - Cellular and Molecular Biology should be useful to anyone currently involved in using transgenesis as a technique - Journal of Heredity Read more...

 
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