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Transgenic animal technology : a laboratory handbook

Author: Carl A Pinkert
Publisher: San Diego : Academic Press, ©2002.
Edition/Format:   Print book : English : 2nd edView all editions and formats
Summary:

Includes chapters on nuclear transfer technologies in mouse, cattle, sheep and swine and on the production of transgenic poultry and primates. This title contains an entire section on analysis,  Read more...

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Genre/Form: Laboratory manuals
laboratory manuals
Document Type: Book
All Authors / Contributors: Carl A Pinkert
ISBN: 0125571666 9780125571661
OCLC Number: 51761423
Description: xv, 618 pages : illustrations ; 24 cm
Contents: Introduction to transgenic animals / Carl A. Pinkert --
DNA microinjection and transgenic animal production / H.G. Polites and Carl A. Pinkert --
Factors affecting transgenic animal production / Paul A. Overbeek --
Gene targeting in embryonic stem cells: I. History and methodology / Thomas Doetschman --
Gene targeting in embryonic stem cells: II. Conditional technologies / Edmund B. Rucker III, James G. Thomson, and Jorge A. Piedrahita --
Retrovirus-mediated gene transfer / Teoan Kim --
Nuclear transfer technologies / Yukio Tsunoda and Yoko Kato --
Production of transgenic rats / Philip Iannaccone and Vasiliy Galat --
Production of transgenic rabbits / James M. Robl and Amy S. Burnside --
Production of transgenic fish / Richard N. Winn --
Production of transgenic poultry / J.N. Petitte and P.E. Mozdziak --
Production of transgenic swine by DNA microinjection / M.J. Martin and Carl A. Pinkert --
Production of transgenic ruminants by DNA microinjection / H. Niemann, H.H. Döpke, and K.G. Hadeler --
Production of transgenic nonhuman primates / A.W.S. Chan, K.Y. Chong, and G. Schatten --
Nuclear transfer technology in cattle, sheep, and swine / Lesley Paterson, William Ritchie, and Ian Wilmut --
Vector design for transgene expression / L.M. Houdebine, J. Attal, and J.L. Vilotte --
Analysis of transgene integration / Brad T. Tinkle and Gilbert Jay --
PCR optimization for detection of transgene integration / M.H. Irwin, W.K. Pogozelski, and Carl A. Pinkert --
Analysis of transgene expression / Lien Ngo and Gilbert Jay --
Assisted reproductive technologies and embryo culture methods for farm animals / Robert A. Godke, Marina Sansinena, and Curtis R. Youngs --
Databases, internet resources, and genetic nomenclature / Anna V. Anagnostopoulos, Larry E. Mobraaten, and Muriel T. Davisson.
Responsibility: edited by Carl A. Pinkert.

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"A `must have' for every lab involved with transgenic technology."-Andreas Kolb, Hannah Research Institute"The handbook will be a valuable resource for two audiences, one seeking a broad appreciation Read more...

 
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