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Transgenic animal technology : a laboratory handbook.

Author: Carl A Pinkert
Publisher: London : Academic Press, 2002.
Edition/Format:   Musical score : English : 2nd

Includes chapters on nuclear transfer technologies in mouse, cattle, sheep and swine and on the production of transgenic poultry and primates. This title contains an entire section on analysis,  Read more...


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Document Type: Musical Score
All Authors / Contributors: Carl A Pinkert
ISBN: 0125571666 9780125571661
OCLC Number: 56804588
Description: 618 pages
Contents: Section OneOverview.Introduction to transgenic animals.Section TwoTransgenic animal production focusing on the mouse model.Transgenic animal production using DNA microinjection.Factors affecting transgenic animal production.Gene targeting in embryonic stem cells I: History and methodology.Gene targeting in embryonic stem cells II. Conditional technologies.Retrovirus-mediated gene transfer.Nuclear transfer technologies.Section ThreeProduction of transgenic laboratory and domestic animal species.Production of transgenic rats.Production of transgenic rabbits.Production of transgenic fish.Production of transgenic poultry.Production of transgenic swine by DNA Microinjection.Production of transgenic ruminants by DNA Microinjection.Production of transgenic non-human primates.Retrovirus-mediated gene transfer into oocytes.Section Four: Molecular biology, analyses and enabling technologiesVector design for Transgene Expression.Analysis of transgene integration.PCR optimization for Detection of Transgenic Integration.Evaluation of Transgene Expression.Control of gene expression (from standard gain/loss of function to anti-sense to PNAs).Enabling technologies:Cryopreservation of gametes.Enabling technologies:Embryo culture considerations.Databases, Internet Resources and Genetic Nomenclature.


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"A `must have' for every lab involved with transgenic technology."-Andreas Kolb, Hannah Research Institute"The handbook will be a valuable resource for two audiences, one seeking a broad appreciation Read more...

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