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Transgenic wheat, barley and oats : production and characterization protocols

Author: Huw D Jones; P R Shewry
Publisher: New York, NY : Humana Press, ©2009.
Series: Methods in molecular biology (Clifton, N.J.), v. 478.; Springer protocols (Series)
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
The understanding of the physical and genetic structure of plant genomes and the determination of phenotypes via interactions of coding and non-coding regions with the environment has quickly become the key to the future of plant breeding and agriculture. In Transgenic Wheat, Barley and Oats: Production and Characterization Protocols, expert investigators contribute the latest protocols for the transformation,  Read more...
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Genre/Form: Electronic books
Laboratory Manuals
Additional Physical Format: Print version:
Transgenic wheat, barley and oats.
New York, NY : Humana Press, ©2009
(DLC) 2008933591
(OCoLC)227922736
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Huw D Jones; P R Shewry
ISBN: 9781597453790 159745379X
OCLC Number: 300491846
Language Note: English.
Description: 1 online resource (x, 349 pages, [8] pages of plates) : illustrations (some color).
Contents: Transgenic wheat, barley and oats : production and characterization / Paul A. Lazzeri and Huw D. Jones --
Selection of transformed plants / Huw D. Jones and Caroline A. Sparks --
Reporter genes / Alison Huttly --
Biolistics transformation of wheat / Caroline A. Sparks and Huw D. Jones --
Agrobacterium-mediated transformation of bread and durum wheat using freshly isolated immature embryos / Huixia Wu, Angela Doherty and Huw D. Jones --
Floral transformation of wheat / Sujata Agarwal [and others] --
Highly efficient agrobacterium-mediated transformation of wheat via in planta inoculation / Thierry Risacher [and others] --
Barley transformation using biolistic techniques / Wendy A. Harwood and Mark A. Smedley --
Barley transformation using agrobacterium-mediated techniques / Wendy A. Harwood [and others] --
Transformation of oats and its application to improving osmotic stress tolerance / Shahina B. Maqbool [and others] --
Promoter sequences for defining transgene expression / Huw D. Jones and Caroline A. Sparks --
Down-regulation of gene expression by RNA-induced gene silencing / Silvia Travella and Beat Keller --
Gene insertion patterns and sites / Philippe Vain and Vera Thole --
Fluorescent in situ hybridization to detect transgene integration into plant genomes / Trude Schwarzacher --
Establishing substantial equivalence : transcriptomics / María Marcela Baudo [and others] --
Establishing substantial equivalence : proteomics / Alison Lovegrove, Louise Salt, and Peter R. Shewry --
Establishing substantial equivalence : metabolomics / Michael H. Beale, Jane L. Ward, and John M. Baker --
Design and management of field trials of transgenic cereals / Zoltán Bedő, Mariann Rakszegi, and László Láng --
GM risk assessment / Penny A.C. Sparrow --
Transgenic wheat, barley and oats : future prospects / Jim M. Dunwell.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 478.; Springer protocols (Series)
Responsibility: edited by Huw D. Jones and Peter R. Shewry.

Abstract:

Here, experts examine the latest protocols for the transformation, regeneration and selection of these three species, using both biolistics and Agrobacterium tumefaciens, to fulfil the need for  Read more...

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