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Translocation : movement of tRNA and mRNA through the ribosome

Author: Harry F Noller
Publisher: London : Henry Stewart Talks, 2008.
Series: Henry Stewart talks., Biomedical & life sciences collection., Mechanisms of ribosome function : insights into protein synthesis.
Edition/Format:   eVideo : Clipart/images/graphics : EnglishView all editions and formats
Database:WorldCat
Summary:
Audio-visual presentation: Ribosomes and translation -- Molecular composition of the ribosome -- Why is the ribosome so big? -- The mRNA channel in the 30S subunit -- Positions of tRNA in the A-, P- and E-sites -- Structural dynamics of the ribosome during translocation -- The hybrid states elongation cycle -- tRNA binding during its movement through the ribosome -- The 70S ribosome -- Distortion of tRNA structure  Read more...
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Details

Material Type: Clipart/images/graphics, Internet resource, Videorecording
Document Type: Internet Resource, Computer File, Visual material
All Authors / Contributors: Harry F Noller
OCLC Number: 310381880
Notes: Title from title frames.
Animated audio-visual presentation with synchronized narration.
Description: 1 streaming video file (41 min.) : digital, mono, SWF file, sound, color.
Details: Mode of access: World Wide Web.; System requirements: Operating System: PC Windows 2000+, Mac OSX+ 3.2. Browser Compatibility: IE6+, Firefox 2+, Opera 9+, Safari 2+ 3.3. Browser settings: enable JavaScript, enable popups from the Henry Stewart Talks site. 3.4. Required Browser Plugins & Viewers: Adobe (Macromedia) Flash Player 7+, Adobe Acrobat Reader6.0+.
Contents: Ribosomes and translation --
Molecular composition of the ribosome --
Why is the ribosome so big? --
The mRNA channel in the 30S subunit --
Positions of tRNA in the A-, P- and E-sites --
Structural dynamics of the ribosome during translocation --
The hybrid states elongation cycle --
tRNA binding during its movement through the ribosome --
The 70S ribosome --
Distortion of tRNA structure by the ribosome --
The ratchet model --
Cryo-EM experiment --
Inferred relationship between ratcheting and hybrid states --
FRET changes --
Intersubunit movement --
Testing the ratchet model for translocation --
Translocation assay --
What defines the mechanism of translocation --
Sparsomycin --
The energetics of translocation --
E-site tRNA --
The ribosome is a molecular machine.
Series Title: Henry Stewart talks., Biomedical & life sciences collection., Mechanisms of ribosome function : insights into protein synthesis.
Responsibility: Harry F. Noller.
More information:

Abstract:

Audio-visual presentation: Ribosomes and translation -- Molecular composition of the ribosome -- Why is the ribosome so big? -- The mRNA channel in the 30S subunit -- Positions of tRNA in the A-, P- and E-sites -- Structural dynamics of the ribosome during translocation -- The hybrid states elongation cycle -- tRNA binding during its movement through the ribosome -- The 70S ribosome -- Distortion of tRNA structure by the ribosome -- The ratchet model -- Cryo-EM experiment -- Inferred relationship between ratcheting and hybrid states -- FRET changes -- Intersubunit movement -- Testing the ratchet model for translocation -- Translocation assay -- What defines the mechanism of translocation -- Sparsomycin -- The energetics of translocation -- E-site tRNA -- The ribosome is a molecular machine.

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