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Type-1 diabetes : methods and protocols

Author: Kathleen M Gillespie
Publisher: New York, NY : Humana Press, 2016.
Series: Methods in molecular biology (Clifton, N.J.), v. 1433.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on  Read more...
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Genre/Form: Electronic books
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Kathleen M Gillespie
ISBN: 9781493936434 1493936433 1493936417 9781493936410
OCLC Number: 951536404
Notes: Includes index.
Description: 1 online resource (x, 213 pages) : illustrations (some color).
Contents: Type 1 diabetes: Current perspectives --
Type 1 diabetes high-risk HLA class ii determination by polymerase chain reaction sequence-specific primers --
Pancreatic beta cell survival and signaling pathways: Effects of type 1 diabetes-associated genetic variants --
Islet autoantibody analysis: Radioimmunoassays --
Islet autoantibody detection by electrochemiluminescence (ECL) assay --
Detection of C-peptide in urine as a measure of ongoing beta cell function --
Histology of type 1 diabetes pancreas --
Identification of islet antigen-specific CD8 T cells using mhci-peptide tetramer reagents in the non obese diabetic (NOD) mouse model of type 1 diabetes --
Tracking immunological responses of islet antigen-specific T cells in the nonobese diabetic (NOD) mouse model of type 1 diabetes --
Adoptive transfer of autoimmune diabetes using immunodeficient nonobese diabetic (NOD) mice --
Methylation analysis in distinct immune cell subsets in type 1 diabetes --
Fluorescence in situ hybridization with concomitant immunofluorescence in human pancreas --
Laser capture and single cell genotyping from frozen tissue sections --
The gut microbiome in the NOD mouse --
Molecular methods and protein synthesis for definition of autoantibody epitopes.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1433.
Other Titles: Type 1 diabetes
Type one diabetes
Responsibility: edited by Kathleen M. Gillespie.

Abstract:

The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.

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