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Type 3 secretion systems : methods and protocols

Author: Matthew L Nilles; Danielle Jessen Condry
Publisher: New York, NY : Humana Press, [2016] ©2017
Series: Methods in molecular biology (Clifton, N.J.), 1531.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems  Read more...

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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: (OCoLC)954425099
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Matthew L Nilles; Danielle Jessen Condry
ISBN: 9781493966493 1493966499
OCLC Number: 963832100
Notes: Includes index.
Description: 1 online resource (x, 224 pages) : illustrations (some color).
Contents: Preface Foreword Table of Contents Contributing Authors1. Introduction to Type III Secretion SystemsDanielle Condry and Matthew L. Nilles2. Site-Directed Mutagenesis and its Application in Studying the Interactions of T3S ComponentsMatthew S. Francis, Ayad A.A. Amer, Debra L. Milton, and Tiago R.D. Costa3. Blue Native Protein Electrophoresis to Study the T3S System using Yersinia pestis as a ModelThomas A. Henderson and Matthew L. Nilles4. In Vivo Photo-Cross-Linking to Study T3S Interactions Demonstrated using the Yersinia pestis T3S SystemThomas A. Henderson and Matthew L. Nilles5. Isolation of Type III Secretion System Needle Complexes by ShearingMatthew L. Nilles, Danielle L. Jessen Condry, and Patrick Osei-Owusu6. Use of Transcriptional Control to Increase Expression and Secretion of Heterologous Proteins in T3S SystemsKevin J. Metcalf and Danielle Tullman-Ercek7. Characterization of Type Three Secretion System Translocator Interactions with Phospholipid MembranesPhilip R. Adam, Michael L. Barta, and Nicholas E. Dickenson8. Analysis of Type III Secretion System Secreted ProteinsDanielle L. Jessen Condry and Matthew L. Nilles9. Fractionation Techniques to Examine Effector TranslocationRachel M. Olson and Deborah M. Anderson10. Measurement of Effector Protein Translocation using Phosphorylatable Epitope Tags and Phospho-Specific AntibodiesSara Schesser Bartra and Gregory V. Plano11. A TAL-Based Reporter Assay for Monitoring Type III-Dependent Protein Translocation in XanthomonasSabine Drehkopf, Jens Hausner, Michael Jordan, Felix Scheibner, Ulla Bonas, and Daniela Buttner12. Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in PlantsKyaw Aung, Xiufang Xin, Christy Mecey, and Sheng Yang He13. A Method for Characterizing the Type III Secretion System's Contribution to Pathogenesis: Homologous Recombination to Generate Yersinia pestis Type III Secretion System MutantsPatrick Osei-Owusu, Matthew L. Nilles, David S. Bradley, and Travis D. Alvine14. Detecting Immune Responses to Type III Secretion SystemsPeter L. Knopick and David S. Bradley15. Recombinant Expression and Purification of the Shigella Translocator IpaBMichael L. Barta, Philip R. Adam, and Nicholas E. Dickenson16. Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion ProteinsTravis D. Alvine, atrick Osei-Owusu, Danielle L. Jessen Condry, and Matthew L. Nilles17. Mouse Immunization with Purified Needle Proteins from Type III Secretion Systems and the Characterization of the Immune Response to these ProteinsTravis D. Alvine, David S. Bradley, and Matthew L. Nilles18. Identification of the Targets of Type III Secretion System InhibitorsDanielle L. Jessen Condry and Matthew L. Nilles19. Detection of Protein Interactions in T3S Systems using Yeast 2-Hybrid AnalysisMatthew L. Nilles
Series Title: Methods in molecular biology (Clifton, N.J.), 1531.
Responsibility: edited by Matthew L. Nilles, Danielle L. Jessen Condry.

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