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Ubiquitin-proteasome protocols

Author: Cam Patterson; Douglas M Cyr
Publisher: Totowa, N.J. : Humana Press, ©2005.
Series: Methods in molecular biology (Clifton, N.J.), v. 301.
Edition/Format:   Print book : EnglishView all editions and formats
Database:WorldCat
Summary:

Presents a collection of techniques for studying ubiquitin dependent protein degradation via the proteasome. This work covers topics from basic biochemistry to cellular assays to discovery techniques  Read more...

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Genre/Form: Laboratory manuals
Aufsatzsammlung
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Cam Patterson; Douglas M Cyr
ISBN: 1588292525 9781588292520 1592598951 9781592598953
OCLC Number: 56066486
Description: xi, 381 pages : illustrations ; 24 cm.
Contents: Small-molecule inhibitors of proteasome activity / Maria Gaczynska and Pawel A. Osmulski --
Purification of E1 and E1-like enzymes / Arthur L. Haas --
Assays for RING family ubiquitin ligases / Manabu Furukawa, Paul S. Andrews, and Yue Xiong --
Ubiquitin chain synthesis / Shahri Raasi and Cecile M. Pickart --
Purification of proteasomes, proteasome subcomplexes, and proteasome-associated proteins from budding yeast / David S. Leggett, Michael H. Glickman, and Daniel Finley --
Recognition and processing of misfolded proteins by PA700, the 19S regulatory complex of the 26S proteasome / Chang-Wei Liu [and others] --
Cell-free assay for ubiquitin-independent proteasomal protein degradation / Chaim Kahana and Yuval Reiss. Assays of proteasome-dependent cleavage products / Stefan Tenzer and Hansjörg Schild --
Identification of components of protein complexes / Carol E. Parker [and others] --
Mass spectrometric determination of protein ubiqitination / Carol E. Parker [and others] --
Reconstitution of endoplasmic reticulum-associated degradation using yeast membranes and cytosol / Robert J. Lee, Ardythe A. McCracken, and Jeffrey L. Brodsky --
Reticulocyte lysate as a model system to study endoplasmic reticulum membrane protein degradation / Eric Carlson [and others] --
Deubiquitinating enzyme purifucation, assay inhibitors, and characterization / Nathaniel S. Russell and Keith D. Wilkinson --
Measuring ubiquitin conjugation in cells / Edward G. Mimnaugh and Leonard M. Neckers --
Assays for proteasome assembly and maturation / R. Jürgen Dohmen [and others]. N-terminal ubiquitination / Aaron Ciechanover --
Quantitating defective ribosome products / Shu-Bing Qian, Jack R. Bennink, and Jonathan W. Yewdell --endoplasmic reticulum-associated protein quality control and degradation: screen for ERAD mutants after ethylmethane sulfonate mutagenesis / Antje Schäfer and Dieter H. Wolf --
Endoplasmic reticulum-associated protein quality control and degradation: genome wide screen for ERAD components / Antje Schäfer and Dieter H. Wolf --
Cystic fibrosis transmembrane conductance regulator as a model substrate to study endoplasmic reticulum protein quality control in mammalian cells / J. Michael Younger [and others] --
Aggresome formation / Michael J. Corboy, Philip J. Thomas, and W. Christian Wigley --
Detection of sumoylated proteins / Roland S. Hilgarth and Kevin D. Sarge --
Proteasome inhibitors in cancer therapy / Robert Z. Orlowski --
Parkinson's disease: assays for the ubiquitin ligase activity of neural parkin / Michael G. Schlossmacher and Hideki Shimura.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 301.
Responsibility: edited by Cam Patterson, Douglas M. Cyr.
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"...clearly written and thorough...a valuable addition to any lab entering, or already working in, the ubiquitin or proteasome field." - Doody's Health Science Book Review Journal

 
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