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UHPLC in life sciences

Author: Davy Guillarme; Jean-Luc Veuthey; Royal Society of Chemistry (Great Britain)
Publisher: Cambridge, U.K. : Royal Society of Chemistry, 2012.
Series: RSC chromatography monographs, 16.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Database:WorldCat
Summary:
Since its commercial introduction in 2004, UHPLC (Ultra-High Performance Liquid Chromatography) has begun to replace conventional HPLC in academia and industry and interest in this technique continues to grow. Both the increases in speed and resolution make this an attractive method; particularly to the life sciences and more than 1500 papers have been written on this strongly-evolving topic to date.
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Genre/Form: Electronic books
Additional Physical Format: Print version:
UHPLC in life sciences.
Cambridge : Royal Society of Chemistry, 2012
(OCoLC)779245884
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Davy Guillarme; Jean-Luc Veuthey; Royal Society of Chemistry (Great Britain)
ISBN: 9781849735490 1849735492 9781621981466 1621981460
OCLC Number: 798102383
Description: 1 online resource (xviii, 447 pages) : illustrations.
Contents: 1.1. Trends in HPLC; 1.2. Comparison of Chromatographic Techniques and Supports; 1.3. Constructing Kinetic Plots; 1.4. History of the Kinetic Plot Method; 1.5 Unification of the Isocratic and Gradient Kinetic Plot Method; 1.6. Relation between the Kinetic Performance under Isocratic and Gradient Elution Conditions; 1.7. Influence of the Test Conditions on the Obtained Kinetic-Performance Limit Curve; 1.8. Some Reflections on Recent Trends in Liquid Chromatography using the Kinetic Plot Method; 1.9. Conclusions; Acknowledgements; References; 2.1 UHPLC Instrumentation; 2.2 UHPLC Columns; 3.1 Introduction; 3.2 Qualitative Transfer from HPLC to UHPLC; 3.3 Normative Context for the HPLC to UHPLC Transfer; 3.4 Validation of UHPLC Methods and Equivalence of the HPLC-UHPLC Methods; 3.5 Conclusions; 3.6 References; 4.1 Introduction; 4.2 High throughput and high resolution in HT-UHPLC; 4.3 Limitations of HTLC and HT-UHPLC; 4.4 Advantage of high temperature in life science analysis; 4.5 HT-UHPLC in comprehensive on-line two-dimensional liquid chromatography (LC x LC); 4.6 Conclusion; 5. Comparison of the performance of totally porous and core-shell particles; 5.1. Introduction; 5.2. Column performance; 5.3. Possibilities of recent core-shell technology; 5.4. Particle size distribution and roughness of core-shell particles; 5.5. Loading capacity of core-shell particles; 5.6. Limited efficiency when core-shell particles packed in narrow-bore columns; 5.7. Extra column effects, contribution to band broadening; 5.8. Performance of core-shell and totally porous particles in isocratic elution mode; 5.9. Performance of core-shell and totally porous particles in gradient elution mode; 5.10. Conclusion; 6.1 Introduction; 6.2 Analytical Conditions for performing HILIC.; 6.3 Applications of HILIC in UHPLC.; 6.4 References.; 7.1 Introduction; 7.2 Selection of ionization techniques; 7.3 Overview of mass analyzers and their main features; 7.4 New developments in mass spectrometry applicable in UHPLC/MS; 7.5 Conclusions; 8.1 Introduction; 8.2 Solubility; 8.3 Ionization; 8.4 Lipophilicity; 8.5 Permeability; 8.6 Conclusion; 8.7 References; 9.1 Introduction; 9.2 UHPLC in Bioanalysis; 9.3 Sample preparation for UHPLC in bioanalysis; 9.4 Conclusions; 10.1 Introduction;10.2 GC-MS Analyses; 10.3 LC-MS(/MS) Analyses; 10.4 Application of UHPLC-MS(/MS) for Drug Testing in Sports; 10.5 Conclusion; References; 11.1 Introduction; 11.2 Use of UHPLC for the Analysis of Seized Drugs; 12.1. Pharmaceuticals as environmental contaminants; 12.2. Analysis of pharmaceuticals in environmental samples; 12.3. Occurrence of pharmaceuticals in environmental and wastewater samples; 12.4. Conclusions; Acknowledgements; References; 13.1 Introduction; 13.2 Multiple Facets of UHPLC in NP research; 13.3 Fast Targeted Analysis; 13.4 Fast Non-Targeted Analysis, Fingerprinting, and Metabolomics; 13.5 High-Resolution Profiling and Metabolite ID; 13.6 Conclusion; Acknowledgments; References; 14.1. Introduction; 14.2. Pre-analysis Considerations: Protocol Design, Sample Collection, Storage and Preparation; 14.3. Sample Preparation for Serum and/Plasma; 14.4. Collection and Storage of Urine Samples; 14.5. UHPLC-MS-Based Metabolite Profiling; 14.6. Applications of UHPLC-MS to Human Metabolic Profiling Studies; 14.7 Current Challenges for UHPLC-MS in Global metabolic profiling Studies; 14.8. Conclusions
Series Title: RSC chromatography monographs, 16.
Other Titles: Ultra-high performance liquid chromatography in life sciences
Responsibility: edited by Davy Guillarme and Jean-Luc Veuthey.

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This book presents a comprehensive overview of UHPLIC and is essential reading to newcomers to field and postgradutes and an essential handbook for experienced users of the technique.  Read more...

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"The book gives a very comprehensive overview of the fundamental and application of ultra high pressure liquid chromatography." "... has a clear layout, good quality of figures and can be recommended Read more...

 
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