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Unnatural amino acids : methods and protocols

Author: Loredano Pollegioni; Stefano Servi
Publisher: [Totowa, NJ] : Humana Press ; New York : Springer Science+Business Media, LLC, ©2012.
Series: Methods in molecular biology (Clifton, N.J.), v. 794.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Even though they are present in nature, non-proteinogenic amino acids are usually defined as unnatural or non-natural. Beside their structural diversity, interest in these compounds is due to their occurrence in nature, their biological properties, the analytical aspects, their use as probes, and their incorporation into peptides and proteins, among other reasons. Divided into five convenient sections, Unnatural  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Printed edition:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Loredano Pollegioni; Stefano Servi
ISBN: 9781617793318 1617793310 9781617793301 1617793302
OCLC Number: 756512314
Description: 1 online resource (xiv, 409 pages) : illustrations.
Contents: Preparation of unnatural amino acids with ammonia-lyases and 2,3-aminomutases --
Multistep enzyme catalyzed reactions for unnatural amino acids --
Enzymatic production of enantiopure amino acids from mono-substituted hydantoin substrates --
Preparation of glutamate analogues by enzymatic transamination --
Carbon-carbon bond-forming enzymes for the synthesis of non-natural amino acids --
Engineering cyclic amidases for non-natural amino acid synthesis --
NMR analysis of unnatural amino acids in natural antibiotics --
Site-specific incorporation of unnatural amino acids as probes for protein conformational changes --
Application of unnatural amino acids to the de novo design of selective antibiotic peptides --
Use of unnatural amino acids to probe structure-activity relationships and mode-of-action of antimicrobial peptides --
Experimental methods for scanning unnatural amino acid mutagenesis --
Genetic incorporation of unnatural amino acids into proteins in yeast --
Site-specific incorporation of unnatural amino acids into proteins in mammalian cells --
Incorporation of unnatural non-alpha-amino acids into the N-terminus of proteins in a cell-free translation system --
Site-specific modification of proteins by the staudinger-phosphite reaction --
Hplc methods for determination of D-aspartate and N-methyl-D-aspartate --
Estimation of chronological age from the racemization rate of L- and D-aspartic acid: How to completely separate enantiomers from dentin --
Enzymatic detection of D-amino acids --
An enzymatic-HPLC assay to monitor endogenous D-serine release from neuronal cultures --
Electrophysiological analysis of the modulation of NMDA-receptors function by D-serine and glycine in the central nervous system --
Biosensors for d-amino acid detection --
Analysis of D-[beta]-aspartyl isomers at specific sites in proteins --
Nutritional value of D-amino acids, d-peptides, and amino acid derivatives in mice --
Preparation and assay of recombinant serine racemase --
Assay of amino acid racemases --
Assays of D-amino acid oxidases --
Enzymes acting on D-amino acid containing peptides.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 794.
Responsibility: edited by Loredano Pollegioni, Stefano Servi.

Abstract:

Divided into convenient sections, this book contributes to directing the reader's attention to the many fields of growing scientific interest in non-natural amino acids. It includes methods and  Read more...

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