skip to content
Unnatural amino acids : methods and protocols Preview this item
ClosePreview this item
Checking...

Unnatural amino acids : methods and protocols

Author: Loredano Pollegioni; Stefano Servi
Publisher: [Totowa, NJ] : Humana Press ; New York : Springer Science+Business Media, LLC, ©2012.
Series: Methods in molecular biology (Clifton, N.J.), v. 794.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Even though they are present in nature, non-proteinogenic amino acids are usually defined as unnatural or non-natural. Beside their structural diversity, interest in these compounds is due to their occurrence in nature, their biological properties, the analytical aspects, their use as probes, and their incorporation into peptides and proteins, among other reasons. Divided into five convenient sections, Unnatural  Read more...
Rating:

(not yet rated) 0 with reviews - Be the first.

Subjects
More like this

Find a copy online

Links to this item

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Genre/Form: Electronic books
Additional Physical Format: Printed edition:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Loredano Pollegioni; Stefano Servi
ISBN: 9781617793318 1617793310 9781617793301 1617793302
OCLC Number: 756512314
Language Note: English.
Description: 1 online resource (xiv, 409 pages) : illustrations.
Contents: Preparation of unnatural amino acids with ammonia-lyases and 2,3-aminomutases --
Multistep enzyme catalyzed reactions for unnatural amino acids --
Enzymatic production of enantiopure amino acids from mono-substituted hydantoin substrates --
Preparation of glutamate analogues by enzymatic transamination --
Carbon-carbon bond-forming enzymes for the synthesis of non-natural amino acids --
Engineering cyclic amidases for non-natural amino acid synthesis --
NMR analysis of unnatural amino acids in natural antibiotics --
Site-specific incorporation of unnatural amino acids as probes for protein conformational changes --
Application of unnatural amino acids to the de novo design of selective antibiotic peptides --
Use of unnatural amino acids to probe structure-activity relationships and mode-of-action of antimicrobial peptides --
Experimental methods for scanning unnatural amino acid mutagenesis --
Genetic incorporation of unnatural amino acids into proteins in yeast --
Site-specific incorporation of unnatural amino acids into proteins in mammalian cells --
Incorporation of unnatural non-alpha-amino acids into the N-terminus of proteins in a cell-free translation system --
Site-specific modification of proteins by the staudinger-phosphite reaction --
Hplc methods for determination of D-aspartate and N-methyl-D-aspartate --
Estimation of chronological age from the racemization rate of L- and D-aspartic acid: How to completely separate enantiomers from dentin --
Enzymatic detection of D-amino acids --
An enzymatic-HPLC assay to monitor endogenous D-serine release from neuronal cultures --
Electrophysiological analysis of the modulation of NMDA-receptors function by D-serine and glycine in the central nervous system --
Biosensors for d-amino acid detection --
Analysis of D-[beta]-aspartyl isomers at specific sites in proteins --
Nutritional value of D-amino acids, d-peptides, and amino acid derivatives in mice --
Preparation and assay of recombinant serine racemase --
Assay of amino acid racemases --
Assays of D-amino acid oxidases --
Enzymes acting on D-amino acid containing peptides.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 794.
Responsibility: edited by Loredano Pollegioni, Stefano Servi.

Abstract:

Divided into convenient sections, this book contributes to directing the reader's attention to the many fields of growing scientific interest in non-natural amino acids. It includes methods and  Read more...

Reviews

User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.
Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Linked Data


Primary Entity

<http://www.worldcat.org/oclc/756512314> # Unnatural amino acids : methods and protocols
    a schema:CreativeWork, schema:Book, schema:MediaObject ;
    library:oclcnum "756512314" ;
    library:placeOfPublication <http://id.loc.gov/vocabulary/countries/nju> ;
    library:placeOfPublication <http://experiment.worldcat.org/entity/work/data/1020800802#Place/totowa_nj> ; # Totowa, NJ
    library:placeOfPublication <http://dbpedia.org/resource/New_York_City> ; # New York
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/information_science> ; # Information Science
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/information_services> ; # Information Services
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/amino_acids_peptides_and_proteins> ; # Amino Acids, Peptides, and Proteins
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/analytical_diagnostic_and_therapeutic_techniques_and_equipment> ; # Analytical, Diagnostic and Therapeutic Techniques and Equipment
    schema:about <http://id.worldcat.org/fast/807622> ; # Amino acids
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/phenomena_and_processes> ; # Phenomena and Processes
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Thing/biochemistry_general> ; # Biochemistry, general
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/health_&_biological_sciences> ; # Health & Biological Sciences
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Thing/enzymology> ; # Enzymology
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/amino_acid_sequence> ; # Amino Acid Sequence
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/amino_acid_substitution> ; # Amino Acid Substitution
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/protein_engineering> ; # Protein Engineering
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/amino_acids> ; # Amino Acids
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Thing/biochemistry> ; # Biochemistry
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/mutagenesis> ; # Mutagenesis
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/molecular_structure> ; # Molecular Structure
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Thing/life_sciences> ; # Life sciences
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/biochemical_phenomena> ; # Biochemical Phenomena
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/animal_biochemistry> ; # Animal Biochemistry
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/genetic_engineering> ; # Genetic Engineering
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/chemical_phenomena> ; # Chemical Phenomena
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/genetic_techniques> ; # Genetic Techniques
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Thing/enzymes> ; # Enzymes
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/investigative_techniques> ; # Investigative Techniques
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/molecular_sequence_data> ; # Molecular Sequence Data
    schema:about <http://dewey.info/class/572.65/e23/> ;
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/human_anatomy_&_physiology> ; # Human Anatomy & Physiology
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/publication_characteristics> ; # Publication Characteristics
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/laboratory_manuals> ; # Laboratory Manuals
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/chemicals_and_drugs> ; # Chemicals and Drugs
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/publication_formats> ; # Publication Formats
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/genetic_phenomena> ; # Genetic Phenomena
    schema:about <http://experiment.worldcat.org/entity/work/data/1020800802#Topic/documentation> ; # Documentation
    schema:bookFormat schema:EBook ;
    schema:contributor <http://viaf.org/viaf/219209286> ; # Loredano Pollegioni
    schema:contributor <http://viaf.org/viaf/58276454> ; # Stefano Servi
    schema:copyrightYear "2012" ;
    schema:datePublished "2012" ;
    schema:description "Even though they are present in nature, non-proteinogenic amino acids are usually defined as unnatural or non-natural. Beside their structural diversity, interest in these compounds is due to their occurrence in nature, their biological properties, the analytical aspects, their use as probes, and their incorporation into peptides and proteins, among other reasons. Divided into five convenient sections, Unnatural Amino Acids: Methods and Protocols deals with enzymatic methods used to produce non-natural amino acids, aspects concerning the presence of unnatural amino acids in peptides with antimicrobial properties, genetic incorporation of unnatural amino acids into proteins (yeast and mammalian cells), and detection and quantification of D-amino acids and related enzymes. Written in the highly successful Methods in Molecular Biology"!series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Unnatural Amino Acids: Methods and Protocols serves as an ideal guide for scientists and contributes to directing the attention of researchers to the many fields of growing scientific interest in non-natural amino acids."@en ;
    schema:description "Preparation of unnatural amino acids with ammonia-lyases and 2,3-aminomutases -- Multistep enzyme catalyzed reactions for unnatural amino acids -- Enzymatic production of enantiopure amino acids from mono-substituted hydantoin substrates -- Preparation of glutamate analogues by enzymatic transamination -- Carbon-carbon bond-forming enzymes for the synthesis of non-natural amino acids -- Engineering cyclic amidases for non-natural amino acid synthesis -- NMR analysis of unnatural amino acids in natural antibiotics -- Site-specific incorporation of unnatural amino acids as probes for protein conformational changes -- Application of unnatural amino acids to the de novo design of selective antibiotic peptides -- Use of unnatural amino acids to probe structure-activity relationships and mode-of-action of antimicrobial peptides -- Experimental methods for scanning unnatural amino acid mutagenesis -- Genetic incorporation of unnatural amino acids into proteins in yeast -- Site-specific incorporation of unnatural amino acids into proteins in mammalian cells -- Incorporation of unnatural non-alpha-amino acids into the N-terminus of proteins in a cell-free translation system -- Site-specific modification of proteins by the staudinger-phosphite reaction -- Hplc methods for determination of D-aspartate and N-methyl-D-aspartate -- Estimation of chronological age from the racemization rate of L- and D-aspartic acid: How to completely separate enantiomers from dentin -- Enzymatic detection of D-amino acids -- An enzymatic-HPLC assay to monitor endogenous D-serine release from neuronal cultures -- Electrophysiological analysis of the modulation of NMDA-receptors function by D-serine and glycine in the central nervous system -- Biosensors for d-amino acid detection -- Analysis of D-[beta]-aspartyl isomers at specific sites in proteins -- Nutritional value of D-amino acids, d-peptides, and amino acid derivatives in mice -- Preparation and assay of recombinant serine racemase -- Assay of amino acid racemases -- Assays of D-amino acid oxidases -- Enzymes acting on D-amino acid containing peptides."@en ;
    schema:exampleOfWork <http://worldcat.org/entity/work/id/1020800802> ;
    schema:genre "Electronic books"@en ;
    schema:inLanguage "en" ;
    schema:isPartOf <http://worldcat.org/issn/1064-3745> ; # Methods in molecular biology (Clifton, N.J.) ;
    schema:isSimilarTo <http://worldcat.org/entity/work/data/1020800802#CreativeWork/> ;
    schema:name "Unnatural amino acids : methods and protocols"@en ;
    schema:productID "756512314" ;
    schema:publication <http://www.worldcat.org/title/-/oclc/756512314#PublicationEvent/totowa_nj_humana_press_new_york_springer_science+business_media_llc_2012> ;
    schema:publisher <http://experiment.worldcat.org/entity/work/data/1020800802#Agent/springer_science+business_media_llc> ; # Springer Science+Business Media, LLC
    schema:publisher <http://experiment.worldcat.org/entity/work/data/1020800802#Agent/humana_press> ; # Humana Press
    schema:url <http://www.springerprotocols.com/doiresolver?genre=book&pid=10.1007/978-1-61779-331-8> ;
    schema:url <http://www.library.yorku.ca/e/resolver/id/2369847> ;
    schema:url <http://proxy.library.carleton.ca/login?url=http://dx.doi.org/10.1007/978-1-61779-331-8> ;
    schema:url <http://dx.doi.org/10.1007/978-1-61779-331-8> ;
    schema:url <https://0-link-springer-com.pugwash.lib.warwick.ac.uk/book/10.1007/978-1-61779-331-8> ;
    schema:url <http://link.springer.com/10.1007/978-1-61779-331-8> ;
    schema:url <https://link.springer.com/openurl?genre=book&isbn=978-1-61779-330-1> ;
    schema:url <http://proxy.library.carleton.ca/login?url=http://books.scholarsportal.info/viewdoc.html?id=/ebooks/ebooks2/springer/2012-07-23/1/9781617793318> ;
    schema:url <http://link.springer.com/openurl?genre=book&isbn=978-1-61779-330-1> ;
    schema:url <http://link.springer.com/openurl?genre=book&isbn=978-1-61779-331-8> ;
    schema:url <https://link.springer.com/book/10.1007/978-1-61779-331-8> ;
    schema:workExample <http://worldcat.org/isbn/9781617793318> ;
    schema:workExample <http://dx.doi.org/10.1007/978-1-61779-331-8> ;
    schema:workExample <http://worldcat.org/isbn/9781617793301> ;
    wdrs:describedby <http://www.worldcat.org/title/-/oclc/756512314> ;
    .


Related Entities

<http://dbpedia.org/resource/New_York_City> # New York
    a schema:Place ;
    schema:name "New York" ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Agent/springer_science+business_media_llc> # Springer Science+Business Media, LLC
    a bgn:Agent ;
    schema:name "Springer Science+Business Media, LLC" ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Thing/biochemistry_general> # Biochemistry, general
    a schema:Thing ;
    schema:name "Biochemistry, general" ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/amino_acid_sequence> # Amino Acid Sequence
    a schema:Intangible ;
    schema:name "Amino Acid Sequence"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/amino_acid_substitution> # Amino Acid Substitution
    a schema:Intangible ;
    schema:name "Amino Acid Substitution"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/amino_acids_peptides_and_proteins> # Amino Acids, Peptides, and Proteins
    a schema:Intangible ;
    schema:name "Amino Acids, Peptides, and Proteins"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/analytical_diagnostic_and_therapeutic_techniques_and_equipment> # Analytical, Diagnostic and Therapeutic Techniques and Equipment
    a schema:Intangible ;
    schema:name "Analytical, Diagnostic and Therapeutic Techniques and Equipment"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/animal_biochemistry> # Animal Biochemistry
    a schema:Intangible ;
    schema:name "Animal Biochemistry"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/biochemical_phenomena> # Biochemical Phenomena
    a schema:Intangible ;
    schema:name "Biochemical Phenomena"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/chemical_phenomena> # Chemical Phenomena
    a schema:Intangible ;
    schema:name "Chemical Phenomena"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/chemicals_and_drugs> # Chemicals and Drugs
    a schema:Intangible ;
    schema:name "Chemicals and Drugs"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/genetic_engineering> # Genetic Engineering
    a schema:Intangible ;
    schema:name "Genetic Engineering"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/genetic_phenomena> # Genetic Phenomena
    a schema:Intangible ;
    schema:name "Genetic Phenomena"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/genetic_techniques> # Genetic Techniques
    a schema:Intangible ;
    schema:name "Genetic Techniques"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/health_&_biological_sciences> # Health & Biological Sciences
    a schema:Intangible ;
    schema:name "Health & Biological Sciences"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/human_anatomy_&_physiology> # Human Anatomy & Physiology
    a schema:Intangible ;
    schema:name "Human Anatomy & Physiology"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/information_science> # Information Science
    a schema:Intangible ;
    schema:name "Information Science"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/information_services> # Information Services
    a schema:Intangible ;
    schema:name "Information Services"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/investigative_techniques> # Investigative Techniques
    a schema:Intangible ;
    schema:name "Investigative Techniques"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/laboratory_manuals> # Laboratory Manuals
    a schema:Intangible ;
    schema:name "Laboratory Manuals"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/molecular_sequence_data> # Molecular Sequence Data
    a schema:Intangible ;
    schema:name "Molecular Sequence Data"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/molecular_structure> # Molecular Structure
    a schema:Intangible ;
    schema:name "Molecular Structure"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/phenomena_and_processes> # Phenomena and Processes
    a schema:Intangible ;
    schema:name "Phenomena and Processes"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/protein_engineering> # Protein Engineering
    a schema:Intangible ;
    schema:name "Protein Engineering"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/publication_characteristics> # Publication Characteristics
    a schema:Intangible ;
    schema:name "Publication Characteristics"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1020800802#Topic/publication_formats> # Publication Formats
    a schema:Intangible ;
    schema:name "Publication Formats"@en ;
    .

<http://id.worldcat.org/fast/807622> # Amino acids
    a schema:Intangible ;
    schema:name "Amino acids"@en ;
    .

<http://link.springer.com/10.1007/978-1-61779-331-8>
    rdfs:comment "Springer Complete Protocols" ;
    .

<http://viaf.org/viaf/219209286> # Loredano Pollegioni
    a schema:Person ;
    schema:familyName "Pollegioni" ;
    schema:givenName "Loredano" ;
    schema:name "Loredano Pollegioni" ;
    .

<http://viaf.org/viaf/58276454> # Stefano Servi
    a schema:Person ;
    schema:birthDate "1943" ;
    schema:familyName "Servi" ;
    schema:givenName "Stefano" ;
    schema:name "Stefano Servi" ;
    .

<http://worldcat.org/entity/work/data/1020800802#CreativeWork/>
    a schema:CreativeWork ;
    schema:description "Printed edition:" ;
    schema:isSimilarTo <http://www.worldcat.org/oclc/756512314> ; # Unnatural amino acids : methods and protocols
    .

<http://worldcat.org/isbn/9781617793301>
    a schema:ProductModel ;
    schema:isbn "1617793302" ;
    schema:isbn "9781617793301" ;
    .

<http://worldcat.org/isbn/9781617793318>
    a schema:ProductModel ;
    schema:isbn "1617793310" ;
    schema:isbn "9781617793318" ;
    .

<http://worldcat.org/issn/1064-3745> # Methods in molecular biology (Clifton, N.J.) ;
    a bgn:PublicationSeries ;
    schema:hasPart <http://www.worldcat.org/oclc/756512314> ; # Unnatural amino acids : methods and protocols
    schema:issn "1064-3745" ;
    schema:name "Methods in molecular biology (Clifton, N.J.) ;" ;
    schema:name "Methods in molecular biology," ;
    .

<https://link.springer.com/book/10.1007/978-1-61779-331-8>
    rdfs:comment "Access restricted to Stanford community" ;
    .


Content-negotiable representations

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.