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The use of CRISPR/cas9, ZFNs, TALENs in generating site specific genome alterations. Volume 546

Author: Jennifer A Doudna; Erik J Sontheimer; Carolin Anders
Publisher: Amsterdam : Elsevier/Academic Press, 2014. ©2014
Series: Methods in enzymology, volume 546.
Edition/Format:   eBook : Document : English : First editionView all editions and formats
Summary:
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Use of CRISPR/cas9, ZFNs, TALENs in generating site specific genome alterations.
San Diego, California : Academic Press, ©2014
xx, 549 pages
(OCoLC)885225075
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Jennifer A Doudna; Erik J Sontheimer; Carolin Anders
ISBN: 9780128013342 0128013346 9780128016152 0128016159
OCLC Number: 899004371
Description: 1 online resource (594 pages, 24 unnumbered pages of plates) : illustrations (some color).
Contents: In vitro enzymology of Cas9 --
Targeted genome editing in human cells using CRISPR/Cas nucleases and truncated guide RNAs --
Determining the specificities of TALENs, Cas9, and other genome-editing enzymes --
Genome engineering with custom recombinases --
Genome engineering in human cells --
Genome editing in human stem cells --
Tagging endogenous loci for live-cell fluorescence imaging and molecule counting using ZFNs, TALENs, and Cas9 --
Genome editing using Cas9 nickases --
Assaying break and nick-induced homologous recombination in mammalian cells using the DR-GFP reporter and Cas9 nucleases --
Adapting CRISPR/Cas9 for functional genomics screens --
The iCRISPR platform for rapid genome editing in human pluripotent stem cells --
Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired Nicks --
Genome editing for human gene therapy --
Generation of site-specific mutations in the rate genome via CRISPR/Cas9 --
CRISPR/Cas9-based genome editing in mice by single plasmid injection --
Imaging genomic elements in living cells using CRISPR/Cas9 --
Cas9-based genome editing in xenopus tropicalis --
Cas9-based genome editing in zebrafish --
Cas9-based genome editing in drosophila --
Transgene-free genome editing by germline injection of CRISPR/Cas RNA --
Cas9-based genome editing in arabidopsis and tobacco --
Multiplex engineering of industrial yeast genomes using CRISPRm --
Protein engineering of Cas9 for enhanced function.
Series Title: Methods in enzymology, volume 546.
Responsibility: edited by Jennifer A. Doudna, Erik J. Sontheimer ; contributors, Carolin Anders [and seventy-six others].

Abstract:

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells.

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