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Vectorial transport of proteins into and across membranes

Author: Alan Michael Tartakoff
Publisher: San Diego : Academic Press, ©1991.
Series: Methods in cell biology, v. 34.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This is the third volume in this series covering protein translocation within and between cells. Volumes 31 and 32, Vesicular Transport, Parts A and B, treated techniques for eukaryotic cells available as of 1989. This volume, Vectorial Transport of Proteins into and across Membranes, brings together new methods and new topics as of 1991. Highlights include use of anti-idiotype antibodies, gene fusions,  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Vectorial transport of proteins into and across membranes.
San Diego : Academic Press, ©1991
(DLC) 64014220
(OCoLC)26163555
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Alan Michael Tartakoff
ISBN: 9780080859323 0080859321 0125641346 9780125641340
OCLC Number: 298460834
Description: 1 online resource (xxi, 438 pages) : illustrations.
Contents: Use of antiidiotype antibodies for the characterization of protein-protein interactions --
In vivo protein translocation into or across the bacterial plasma membrane --
Analysis of membrane protein topology using alkaline Phosphatase and [beta]-Galactosidase gene fusions --
Expression of foreign polypeptides at the Escherichia coli cell surface --
In vitro biochemical studies on translocation of presecretory proteins across the cytoplasmic membrane of Escherichia coli --
Preprotein translocase of Escherichia coli --
In vitro protein translocation into Escherichia coli inverted membrane vesicles --
Membrane components of the protein secretion machinery --
Signal sequence-independent protein secretion in gram-negative bacteria --
Transcription of full-length and truncated mRNA transcripts to study protein translocation across the endoplasmic reticulum --
Probing the molecular environment of translocating polypeptide chains by cross-linking. Reconstitution of secretory protein translocation from detergent-solubilized rough microsomes --
Analysis of protein topology in the endoplasmic reticulum --
Protein import into peroxisomes in vitro --
In vitro reconstitution of protein transport into chloroplasts --
Analysis of mitochondrial protein import using translocation intermediates and specific antibodies --
Import of precursor proteins into yeast submitochondrial particles --
Pulse labeling of yeast cells as a tool to study mitochondrial protein import --
Protein import machinery of yeast mitochondria --
Protein import into isolated yeast mitochondria --
Mitochondrial inner membrane protease I of Saccharomyces cerevisiae --
Purified precursor proteins for studying protein import into yeast mitochondria --
Cross-linking reagents as tools for identifying components of the yeast mitochondrial protein import machinery.
Series Title: Methods in cell biology, v. 34.
Responsibility: edited by Alan M. Tartakoff.

Abstract:

Covering protein translocation within and between cells, this book presents various methods and topics, and information on the use of anti-idiotype antibodies, gene fusions, transcription,  Read more...

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