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| Genre/Form: | Laboratory Manuals Laboratory manuals |
|---|---|
| Document Type: | Book, Computer File |
| All Authors / Contributors: |
Barry W Hicks |
| ISBN: | 9781934115879 1934115878 9781597455596 1597455598 |
| OCLC Number: | 258102282 |
| Description: | xi, 357 p. : ill. + 1 CD-ROM (4 3/4 in.) |
| Details: | System requirements for accompanying CD-ROM: compatible with Windows and Apple Macintosh operating systems. |
| Contents: | 1. Using fluorescent proteins to study poxvirus morphogenesis / Brian M. Ward -- 2. Application of reporter gene imaging for studying human embryonic stem cells in living subjects / Kazim H. Narsinh, Feng Cao, and Joseph C. Wu -- 3. Use of a GFP-PML-expressing cell line as a biosensor for human cytomegalovirus infection / Tomonori Ueno and Kiyoko Ogawa-Goto -- 4. Dual-color imaging of tumor angiogenesis / Robert M. Hoffman -- 5. Use of GFP to analyze morphology, connectivity, and function of cells in the central nervous system / Alan R. Harvey ... [et al.] -- 6. Expression of EGFP by adenovirus-mediated gene transfer in the central nervous system / Ryohei Tomioka -- 7. Adenovirus-mediated delivery of short hairpin RNA (shRNA) mediates efficient gene silencing in terminally differentiated cardiac myocytes / Andreas Rinne ... [et al.] -- 8. Microscopic analysis of adenoviral decontamination using GFP adenovirus with comparable sensitivity to flow cytometry / Michael Grusch ... [et al.] -- 9. Production of multicistronic HIV-1 based lentiviral vectors / Nachimuthu Chinnasamy, James Shaffer, and Dhanalakshmi Chinnasamy -- 10. Using an EGFPmeter to evaluate the lentiviral vector production: tricks and traps / Claude Bagnis, Pascal Bailly, and Sylvie Chapel-Fernandes -- 11. Use of a mcrophage-tropic GFP-tagged human immunodeficiency virus type 1 (HIV-1) to study viral reservoirs / Amanda M. Brown -- 12. GFP-lentiviral vectors targeting for neuroAIDS / Yuanan Lu -- 13. Construction of cell-type specific promoter lentiviruses for optically guiding electrophysiological recordings and for targeted gene delivery Aaron M. Jasnow ... [et al.] -- 14. Selective Protein Expression Within the CNS Using Hybrid Lentivirus / Fredrik Gussing ... [et al.] -- 15. Construction and analysis of alphaherpesviruses expressing green fluorescent protein / Bruce W. Banfield and Gregory A. Bird -- 16. The use of green fluorescent fusion proteins to monitor herpes simplex virus replication / Travis J. Taylor and David M. Knipe -- 17. Modified bovine herpesvirus 1 for protein secretion / Gunther M. Keil -- 18. Identification of viral peptide fragments for vaccine development / Zhanglin Lin, Shuang Li, and Yong Chen -- 19. Use of GFP to investigate expression of plant-derived vaccines / Hong-Ye Li and Mee-Len Chye -- 20. Studies of the role and function of barley stripe mosaic virus encoded proteins in replication and movement using GFP fusions / Sophie Haupt ... [et al.] -- 21. Sendai virus for cancer immunotherapy / Yasuji Ueda, Mamoru Hasegawa, and Yoshikazu Yonemitsu -- 22. Poxvirus tropism for primary human leukocytes and hematopoietic cells / Qigui Yu, Ningjie Hu, and Mario Ostrowski -- 23. Reverse genetics of nipah virus to probe viral pathogenicity / Misako Yoneda ... [et al.] -- 24. Baculovirus-mediated gene transfer into mesenchymal stem cells / Chien-Tai Tsai, Ching-Kuang Chuang, and Yu-Chen Hu. |
| Series Title: | Methods in molecular biology (Clifton, N.J.), v. 515.; Springer protocols. |
| Responsibility: | edited by Barry W. Hicks. |
Reviews
Publisher Synopsis
From the reviews: "One of the most useful modifications that have been made to viruses is the ability to encode tracers such as green fluorescent protein (GFP) ! thereby allowing not only study of the virus itself, but also the behaviour of infected cells. As the title indicates, it is a methods text, giving useful practical details important to those attempting to carry out similar studies." (Christopher Ring, Microbiology Today, November, 2009) "This volume in the Methods in Molecular Biology series is a compilation of laboratory protocols organized into 24 chapters dealing with the diverse viral delivery systems for green fluorescent proteins. ! The book is written primarily for practicing scientists involved in the study of green fluorescent expression in live cells and experimental animals. ! provides a number of updated protocols for the practicing investigator. ! a valuable companion for all researchers involved in the use of green fluorescent proteins in biological systems." (Ruben Mestril, Doody's Review Service, September, 2009) "This book focuses on a very important area of application of green fluorescent protein (GFP) and its variants ! . The significance of viral applications of fluorescent proteins is well covered ! . it will certainly be a valuable source of information and a good basis for laboratory protocols for researchers from all different fields of life sciences." (Johannes Schmid, ChemBioChem, Vol. 10, 2009) "Cover the use of Green Fluorescent Protein (GFP) technology to study aspects of the replication of viruses and the majority of the chapters describe the use of viruses and GFP as research tools in a much wider context. ! I enjoyed the book for the breadth of topics and snapshots of research ! . a more likely home for this book is a university or institute library where its eclectic contents will have a sufficiently wide audience." (Paul Monaghan, Infocus Magazine, Issue 19, September, 2010) Read more...
