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Western blotting : methods and protocols

Author: Biji T Kurien; R Hal Scofield
Publisher: New York, NY : Humana Press, [2015] ©2015
Series: Methods in molecular biology (Clifton, N.J.), v.1312.
Edition/Format:   eBook : Document : EnglishView all editions and formats

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Genre/Form: Electronic books
Laboratory manuals
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Biji T Kurien; R Hal Scofield
ISBN: 9781493926947 1493926942 1493926934 9781493926930
OCLC Number: 910964463
Notes: Includes bibliographical references and index.
Description: 1 online resource (xiv, 509 pages) : illustrations (some color).
Contents: Early days of blotting / Edwin Southern --
Origins of protein blotting / Harry Towbin --
Western blotting : remembrance of things past / W. Neal Burnette --
Simian virus 40 and protein transfer / Jaime Renart --
Western blotting : an introduction / Biji T. Kurien and R. Hal Scofield --
From little helpers to automation / Biji T. Kurien and Harry Towbin --
Spectrophotometric methods to determine protein concentration / J. P. Dean Goldring --
Solubilization of proteins : the importance of lysis buffer choice / Mandy Peach [and three others] --
Simultaneous immunoblotting analysis with activity gel electrophoresis and 2-D gel electrophoresis / Der-Yen Lee and Geen-Dong Chang --
Diffusion blotting : a rapid and simple method for production of multiple blots from a single gel / Ingrid Olsen and Harald G. Wiker --
Multiple immunoblots by passive diffusion of proteins from a single SDS-PAGE gel / Biji T. Kurien and R. Hal Scofield --
Slice blotting / Graeme Lowe --
Localizing proteins by tissue printing / Rafael F. Pont-Lezica --
Dot-immunobinding assay (Dot-Iba) / Sumi Surendran, Annamma Mathai, and Vishnampet Venkataraman Radhakrishnan --
Analysis of antibody clonotype by affinity immunoblotting / Biji T. Kurien and R. Hal Scofield --
Glycosaminoglycan blotting and detection after electrophoresis separation / Nicola Volpi and Francesca Maccari --
Well-based reverse-phase protein array of formalin- fixed paraffin-embedded tissue / Joon-Yong Chung and Stephen M. Hewitt --
Quantitative computerized western blotting in detail / Dalit Talmi-Frank, Charles L. Jaffe, and Gad Baneth --
Cationic electrophoresis and eastern blotting / Engelbert Buxbaum --
Miniaturized blotting system for simultaneous detection of different autoantibodies / Ulrich Canzler [and eight others] --
Proteomic expressional profiling of a paraffin-embedded tissue by multiplex tissue immunoblotting / Joon-Yong Chungand Stephen M. Hewitt --
Post-staining electroblotting for efficient and reliable peptide blotting / Der-Yen Lee and Geen-Dong Chang --
Multistrip western blotting : a tool for comparative quantitative analysis of multiple proteins / Edita Aksamitiene, Jan B. Hoek, and Anatoly Kiyatkin --
Western blotting using PVDF membranes and its downstream applications / Setsuko Komatsu --
Blotting from phastGel to membranes by ultrasound / Joseph Kost and Aharon Azagury --
Western blotting of high and low molecular weight proteins using heat / Biji T. Kurien and R. Hal Scofield --
Membrane strip affinity purification of autoantibodies / Biji T. Kurien --
Strip immunoblotting of multiple antigenic peptides / Biji T. Kurien --
Double-blotting : a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures / Françoise Lasne --
Method for resolution and western blotting of very large proteins using agarose electrophoresis / Marion L. Greaser and Chad M. Warren --
Immunodetection of P-selectin using an antibody to its C-terminal tag / Padmaja Mehta-D'souza --
Improvements and variants of the multiple antigen blot assay-MABA : an immunoenzymatic technique for simultaneous antigen and antibody screening / Oscar Noya [and six others] --
Blotting from immobilized pH gradient gels : application to total cell lysates / Harry Towbin --
Immunoprecipitation : western blot for proteins of low abundance / Edward P. Trieu and Ira N. Targoff --
Native electrophoresis and western blot analysis (NEWeB) : methods and applications / Ioannis N. Manoussopoulos and Mina Tsagris --
Shift-western blotting : separate analysis of protein and DNA from protein-DNA complexes / Matthias Harbers --
Grid-immunoblotting / Olga Yeliosof and R. Hal Scofield --
Detection and quantification of protein-protein interactions by far-western blotting / Joshua A. Jadwin, Bruce J. Mayer, and Kazuya Machida --
Western blot analysis of adhesive interactions under fluid shear conditions : the blot rolling assay / Robert Sackstein and Robert Fuhlbrigge --
Centrifuge blotting / Jinny Paul --
Blotting of coomassie blue-stained proteins from PAGE gels to transparencies / Jinny Paul --
B-cell ELISPOT : for the identification of antigen-specific antibody-secreting cells / Hemangi B. Shah and Kristi A. Koelsch --
T cell ELISPOT : for the identification of specific cytokine-secreting T cells / Kerry M. Leehan and Kristi A. Koelsch --
Membrane microplates for one- and two-color ELISPOT and FLUOROSPOT assays / Alexander E. Kalyuzhny --
SDS-PAGE to immunoblot in one hour / Biji T. Kurien [and three others] --
Single-cell western blotting / Syed M. S. Quadri --
Protein detection by simple western analysis / Valerie M. Harris --
Western blotting using microfluidics / Pothur R. Srinivas --
Two-dimensional gel-based protein standardization verified by western blot analysis / Hisao Haniu [and three others] --
Fingerprint deposition on nitrocellulose and polyvinylidene difluoride membranes using alkaline phosphatase / Biji T. Kurien, Debashish Danda, and R. Hal Scofield --
Other notable protein blotting methods : a brief review / Biji T. Kurien and R. Hal Scofield.
Series Title: Methods in molecular biology (Clifton, N.J.), v.1312.
Responsibility: edited by Biji T. Kurien, R. Hal Scofield.
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