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YAC protocols

Author: David Markie
Publisher: Totowa, N.J. : Humana Press, ©1996.
Series: Methods in molecular biology (Clifton, N.J.), v. 54.
Edition/Format:   Print book : EnglishView all editions and formats
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Genre/Form: Aufsatzsammlung
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: David Markie
ISBN: 0896033139 9780896033139
OCLC Number: 32927182
Description: xii, 378 pages : illustrations ; 23 cm.
Contents: Generation of large insert YAC libraries / Zoia Larin, Anthony P. Monaco, Hans Lehrach --
YAC library storage and transport / John E. Collins, Sheila Hassock, Ian Dunham --
YAC library screening 1. Preparation of hybridization filters and PCR pools / Charlotte G. Cole, John E. Collins, Ian Dunham --
YAC library screening 2. Hybridization and PCR-based screening protocols / Charlotte G. Cole, John E. Collins, Ian Dunham --
Cloning of human telomeres in Saccharomyces cerevisiae / Gillian Bates --
Purification of YAC-containing total yeast DNA / Gary A. Silverman --
Restriction analysis of YACs / Jiannis Ragoussis --
RARE-cleavage analysis of YACs / Shawn P. Iadonato, Andreas Gnirke --
YAC localization by fluorescence in situ hybridization / Gabriele Senger --
Alu-PCR fingerprinting of YACs / Alison Coffey, Simon Gregory, Charlotte G. Cole --
Detection of chimerism in YAC clones / Sandro Banfi, Huda Y. Zoghbi --
Amplification with arbitrary primers / Anna Rienzo, Amy C. Peterson, Nelson B. Freimer --
End rescue from YACs using the vectorette / Donald J. Ogilvie, Louise A. James --
Isolation of YAC ends by plasmid rescue / Gillian Bates --
End-rescue of YAC clone inserts by inverse PCR / Gary A. Silverman --
Covering YAC-cloned DNA with phages and cosmids / Jiannis Ragoussis, Anthony P. Monaco --
Fragmentation and integrative modification of YACs / Jennifer W. McKee-Johnson, Roger H. Reeves --
Targeting mutations to YACs by homologous recombination / Karen Duff, Clare Huxley --
Reconstruction of large genomic segments of DNA by meiotic recombination between YACs / Gary A. Silverman --
Genomic reconstruction by mitotic recombination of YACs / David Markie, Jiannis Ragoussis --
Amplification of the copy number of YACs / Lucy L. Ling, Douglas R. Smith, Donald T. Moir --
Transfer of YAC clones to new yeast hosts / Forrest Spencer, Giora Simchen --
Use of ACEDB as a database for YAC library data management / Ian Dunham, Gareth Ll. Maslen --
YAC transfer into mammalian cells by cell fusion / Nicholas P. Davies, Clare Huxley --
YAC transfer by microinjection / Andreas Schedl, Brenda Grimes, Lluís Montoliu --
Transfection of mammalian cells via lipofection / William M. Strauss --
The isolation of cDNAs by hybridization of YACs to cDNA libraries / Russell G. Snell --
cDNA selection with YACs / Satish Parimoo --
Markers, selection, and media in YAC cloning / David Markie.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 54.
Responsibility: edited by David Markie.

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