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Yeast gene analysis

Author: Ian Stansfield, Dr.; Michael J R Stark
Publisher: Amsterdam ; Boston : Elsevier : Academic Press, 2007.
Series: Methods in microbiology, v. 36.
Edition/Format:   Print book : English : 2nd edView all editions and formats
Summary:

Focusing on Saccharomyces cerevisiae, this second edition covers the major genome-wide strategies such as microarray analysis of transcription, synthetic gene array studies, protein microarrays and  Read more...

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Genre/Form: Fulltext
Illustrations
Internet Resources
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Ian Stansfield, Dr.; Michael J R Stark
ISBN: 9780123694782 0123694787 9780123694799 0123694795
OCLC Number: 166229302
Description: xvi, 700 pages, [12] pages of plates : illustrations (some color) ; 25 cm.
Contents: Introduction to functional analysis in yeastYeast genetics and strain constructionYeast transformationA guided tour to PCR-based genomic manipulations of S. cerevisiae (PCR-targeting)Studying essential genes: generating and using promoter fusions and conditional allelesYeast hybrid approachesArray-based yeast two-hybrid screening for protein-protein interactionsReporter genes and their uses in studying yeast gene expressionTranscript analysis: a microarray approachGFP-based microscopic approaches for whole chromosome analysis in yeastsImmunological methodsMeasuring the proximity of proteins in living cells by fluorescence resonance energy transfer between CFP and YFPIdentification, characterization, and phenotypic analysis of covalently linked cell wall ProteinsYeast protein microarraysSmart genetic screensHigh-throughput strain construction and systematic synthetic lethal Screening in Saccharomyces cerevisiaeChemical genomic tools for understanding gene function and drug actionRNA gene analysisAnalysis of gene function of mitochondriaYeast prions and their analysis in vivoMetabolic control in the eukaryotic cell, a Systems Biology perspectivePhylogenetic footprintingSaccharomyces cerevisiae as a tool for human gene function discoveryBioinformatic prediction of yeast gene functionYeast genetic strain and plasmid collectionsYeast gene analysis: the future
Series Title: Methods in microbiology, v. 36.
Responsibility: edited by Ian Stansfield and Michael J.R. Stark.

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