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Yeast metabolic engineering : methods and protocols

Author: Valeria Mapelli
Publisher: New York, NY : Humana Press, [2014] ©2014
Series: Methods in molecular biology (Clifton, N.J.), v. 1152.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Yeast metabolic engineering.
New York : Humana Press, [2014]
(DLC) 2014936204
(OCoLC)868042164
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Valeria Mapelli
ISBN: 9781493905638 1493905635 1493905627 9781493905621
OCLC Number: 878124530
Description: 1 online resource (x, 316 pages) : illustrations (some color).
Contents: An overview on selection marker genes for transformation of Saccharomyces cerevisiae --
Natural and modified promoters for tailored metabolic engineering of the yeast Saccharomyces cerevisiae --
Tools for genetic engineering of the yeast Hansenula polymorpha --
Molecular tools and protocols for engineering the acid-tolerant yeast Zygosaccharomyces bailii as a potential cell factory --
Strains and molecular tools for recombinant protein production in Pichia pastoris --
Methods for efficient high-throughput screening of protein expression in recombinant pichia pastoris strains --
Synthetic RNA switches for yeast metabolic engineering: Screening recombinant enzyme libraries --
Generation of arming yeasts with active proteins and peptides via cell surface display system: Cell surface engineering, bio-arming technology --
Genetic engineering of industrial Saccharomyces cerevisiae strains using a selection/counter-selection approach --
Evolutionary engineering of yeast --
Determination of a dynamic feeding strategy for recombinant pichia pastoris strains --
Yeast metabolomics: Sample preparation for a GC/MS based analysis --
13CC-based metabolic flux analysis in yeast: The Pichia pastoris case --
Pathway activity profiling (PAPi): A tool for metabolic pathway analysis --
QTL mapping by pooled-segregant whole-genome sequencing in yeast --
Genome-scale metabolic models of yeast, methods for their reconstruction, and other applications --
Model-guided identification of gene deletion targets for metabolic engineering in Saccharomyces cerevisiae --
Patents: A tool to bring innovation from the lab bench to the marketplace.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1152.
Responsibility: edited by Valeria Mapelli.

Abstract:

Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that  Read more...

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