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Yersinia pestis protocols

Author: Rui-fu Yang
Publisher: Singapore : Springer, [2018] ©2018
Series: Springer protocols (Series)
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This protocol book includes common and advanced research protocols for Yersinia pestis research and for its identification, genotyping and source-tracing. This book includes protocols for genomic and transcriptomic analysis, small RNA study, protein-protein interaction, gene deletion. It will be helpful for researchers in this field, graduate students, clinicians, and researchers in infectious diseases, systems  Read more...
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Details

Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Print version :
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Rui-fu Yang
ISBN: 9789811079474 9811079471
OCLC Number: 1038761631
Description: 1 online resource (xi, 290 pages) : illustrations (some color)
Contents: Isolation and identification of Yersinia pestis / Zhizhen Qi, Qingwen Zhang, Yong Jin, Youquan Xin, Haihong Zhao, Xiaoyan Yang, Xiaoqing Xu, and Xuefei Zhang --
Isolation and characterization of Yersinia pestis bacteriophages / Xiangna Zhao --
Extraction of chromosomal DNA and plasmid from Yersinia pestis / Yazhou Zhou --
Extraction of total RNA from Yersinia pestis / Haili Wang and Yanping Han --
Genotyping of Yersinia pestis / Yanjun Li and Yujun Cui --
Genome-wide variation analysis of Yersinia pestis / Chao Yang and Yujun Cui --
Genome-wide detection of expressed sRNAs in Yersinia pestis with RNA-seq / Yanping Han --
Validation of Yersinia pestis sRNAs with nonradioactive-labeled northern blotting / Yanping Han --
Determination of transcription start sites (TSSs) in Yersinia pestis with a primer extension assay / Yanping Han --
Purification of Hfq-associated RNAs with RNA immunoprecipitation (RIP) / Yanping Han --
Overexpression of target sRNAs in tightly controlled multicopy plasmids / Xiaofang Gao, Zizhong Liu, and Yanping Han --
Target validation of sRNA with a GFP reporter gene fusion system / Xiaofang Gao, Zizhong Liu, and Yanping Han --
Inducible gene expression in Yersinia pestis / Juping Zhao and Yicheng Sun --
Analysis of yops secretion by Yersinia pestis type III secretion system / Zongmin Du and Shiyang Cao --
Co-immunoprecipitation analysis for the detection of protein-protein interactions in Yersinia pestis / Shiyang Cao and Zongmin Du --
Detection of type III secretion system needle assembly in Yersinia pestis by cross-linking YscF / Shiyang Cao and Zongmin Du --
Evaluation of protein translocation into eukaryotic cells by Yersinia pestis / Yafang Tan --
Adhesion analysis of Yersinia pestis to host cells / Yafang Tan --
Construction of gene deletion mutants in Yersinia pestis / Wenliang Li, Dan Rong, and Yanping Han --
CRISPR-Cas12a-assisted recombineering in Yersinia pestis / Juping Zhao and Yicheng Sun --
Plasmid curing in Yersinia pestis / Yazhou Zhou --
Genetic regulation analysis of Yersinia pestis / Yiquan Zhang --
Investigation of biofilm formation in Yersinia pestis / Haihong Fang --
Extraction and purification of F1 capsule antigen from Y. pestis / Xiaoyi Wang --
Screening of regulator responses to Yersinia pestis infection with a concatenated tandem array of transcription factor response element (catTFRE) pulldown / Chune Jing --
Animal models for Yersinia pestis / Yujing Bi and Xiaoyi Wang --
Antibiotic resistance evaluation of Yersinia pestis / Jingyu Guo --
PCR for detection and identification of Yersinia pestis / Yong Zhao --
Immunological methods for detection and identification of Yersinia pestis / Yong Zhao --
Analysis of fatty acids in Yersinia pestis by gas chromatography / Yafang Tan --
Evaluation of efficacy of Yersinia pestis vaccines / Xiaoyi Wang --
Online resources for Yersinia pestis / Yanfeng Yan --
Effects of Y. pestis on macrophages / Yujing Bi.
Series Title: Springer protocols (Series)
Responsibility: edited by Ruifu Yang.

Abstract:

This protocol book includes common and advanced research protocols for Yersinia pestis research and for its identification, genotyping and source-tracing. This book includes protocols for genomic and  Read more...

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