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山下敦子

Overview
Works: 16 works in 20 publications in 3 languages and 30 library holdings
Roles: Author
Classifications: KNX1850,
Publication Timeline
Key
Publications about 山下敦子
Publications by 山下敦子
Most widely held works by 山下敦子
Nō no naka no tenshi by V. S Ramachandran( Book )
2 editions published in 2013 in Japanese and held by 6 libraries worldwide
Koseki no jūbako : shoninsha no tame no koseki jitsumu no reshipi by Atsuko Yamashita( Book )
4 editions published between 2010 and 2012 in Japanese and held by 5 libraries worldwide
Kon'in, rikon, konshi zokushō, shinken (kanriken), miseinen kōken by Atsuko Yamashita( Book )
1 edition published in 2015 in Japanese and held by 3 libraries worldwide
Aigokeros : cuadernos patagónicos by Narcisa Hirsch( Book )
1 edition published in 2004 in Spanish and held by 2 libraries worldwide
Yōshi rien, enshi zokushō, tokubetsu yōshi rien by Atsuko Yamashita( Book )
1 edition published in 2014 in Japanese and held by 2 libraries worldwide
Sōsoku, shusshō, ninchi by Atsuko Yamashita( Book )
1 edition published in 2014 in Japanese and held by 2 libraries worldwide
Koseki no madoguchi : Furochato de wakaru todokesho no shinsa ( Book )
1 edition published in 2014 in Japanese and held by 1 library worldwide
Koseki no madoguchi : Furochato de wakaru todokesho no shinsa ( Book )
1 edition published in 2016 in Japanese and held by 1 library worldwide
A large‐scale expression strategy for multimeric extracellular protein complexes using Drosophila S2 cells and its application to the recombinant expression of heterodimeric ligand‐binding domains of taste receptor ( file )
1 edition published in 2017 in English and held by 1 library worldwide
Abstract: Many of the extracellular proteins or extracellular domains of plasma membrane proteins exist or function as homo‐ or heteromeric multimer protein complexes. Successful recombinant production of such proteins is often achieved by co‐expression of the components using eukaryotic cells via the secretory pathway. Here we report a strategy addressing large‐scale expression of hetero‐multimeric extracellular domains of plasma membrane proteins and its application to the extracellular domains of a taste receptor. The target receptor consists of a heterodimer of T1r2 and T1r3 proteins, and their extracellular ligand binding domains (LBDs) are responsible for the perception of major taste substances. However, despite the functional importance, recombinant production of the heterodimeric proteins has so far been unsuccessful. We achieved the successful preparation of the heterodimeric LBD by use of Drosophila S2 cells, which have a high secretory capacity, and by the establishment of a stable high‐expression clone producing both subunits at a comparable level. The method overcame the problems encountered in the conventional transient expression of the receptor protein in insect cells using baculovirus or vector lipofection, which failed in the proper heterodimer production because of the biased expression of T1r3LBD over T1r2LBD. The large‐scale expression methodology reported here may serve as one of the considerable strategies for the preparation of multimeric extracellular protein complexes
Yōshi engumi tokubetsu yōshi engumi by Atsuko Yamashita( Book )
1 edition published in 2014 in Japanese and held by 1 library worldwide
Nyūseki tenseki bunseki kokuseki no tokusō by Atsuko Yamashita( Book )
1 edition published in 2016 in Japanese and held by 1 library worldwide
Koseki no madoguchi : Furochato de wakaru todokesho no shinsa ( Book )
1 edition published in 2015 in Japanese and held by 1 library worldwide
Koseki no dōjō : shoninsha no tame no suteppu appu jissen mondaishū by Atsuko Yamashita( Book )
1 edition published in 2011 in Japanese and held by 1 library worldwide
Koseki no madoguchi : Furochato de wakaru todokesho no shinsa ( Book )
1 edition published in 2014 in Japanese and held by 1 library worldwide
Koseki no madoguchi : Furochato de wakaru todokesho no shinsa ( Book )
1 edition published in 2014 in Japanese and held by 1 library worldwide
Policy networks in educational reforms in Japan by Atsuko Yamashita( Archival Material )
1 edition published in 2001 in English and held by 1 library worldwide
 
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Alternative Names
Yamashita, Atsuko
Yamasita, Atuko
ヤマシタ, アツコ
山下, 厚子
山下敦子
Languages
Japanese (17)
English (2)
Spanish (1)
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