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Works: 271 works in 356 publications in 1 language and 359 library holdings
Publication Timeline
Most widely held works by CALIFORNIA UNIV SAN DIEGO LA JOLLA
Measuring Sleep by Wrist Actigraph ( Book )
4 editions published between 1979 and 1981 in English and held by 3 libraries worldwide
Using a piezo-electric transducer, wrist activity was recorded simultaneously with EEG, EOG, and EMG to obtain 102 recordings -- 39 from hospital patients and 63 from non-patients -- during both Sleep and Wakefulness. On a minute-to-minute basis, wrist activity alone was used to estimate Sleep Time. Blind independent scoring of the EEG-EOG-EMG records was also done for Sleep and Wakefulness. Results from the two Sleep/Wake estimations agreed 94.5% of the minutes. Correlations between the two methods were determined for Total Sleep Period (r=0.90), Total Sleep Time (r=0.89), Total Wake Within Sleep (r=0.70), and number of Mid-Sleep Awakenings (r=0.25). Correlation coefficients were even higher when the 39 patients were excluded from the computations. On the average, the actigraphic method overestimated Sleep Time by 10 minutes. Continuous wrist activity recordings provide simple, inexpensive, but very accurate estimates of Sleep Time. (Author)
Silicide Formation and Schottky Barrier of Rare-Earth Metals on SI ( Book )
3 editions published in 1983 in English and held by 3 libraries worldwide
During this period, our activities included the following: Design and construct mechanical masks for Er (and other thin film) depositions; Completion of the construction of a vacuum annealing furnace. The vacuum chamber is pumped by a turbo-molecular pump and is capable of annealing eight 88) different samples sequentially in one pump-down. The normal operating pressures is <2 x 1- to the -7 torr and the chamber is relatively free from carbon and oxygen contamination; Investigation of annealing Er layers deposited on Si in our new vacuum furnace, and the electronic properties of ErSi2 diodes using mechanical masks, and Acceptance of publication by Applied Physics Letters of our paper entitled Surface Morphology of Erbium Silicides (See Appendix A). (Author)
Characterization of Novel Seven Transmembrane Helix, GS-Coupled Receptors and Pou Domain Proteins in Cancer of the Mammary Epithelium ( Book )
6 editions published between 1995 and 1998 in English and held by 2 libraries worldwide
Understanding the molecular basis of breast carcinoma is central to designing rational and effective therapy for this prevalent disease
Ret Receptor: Functional Consequences of Oncogenic Rearrangements ( Book )
6 editions published between 1995 and 1998 in English and held by 2 libraries worldwide
The ret/ptc2 gene was cloned from human papillary thyroid carcinomas, and is the product of a reciprocal chromosomal rearrangement, translocation event between the cAMP dependent protein kinase regulatory subunit I alpha (RI alpha) and the tyrosine kinase domain of the Ret receptor. Ret/ptc2 is a dimer which is autophosphorylated, soluble, and constitutively active. We generated a computer model of the Ret/ptc2 kinase domain, expressed and purified Ret/ptc2, developed a kinase assay to test a variety of peptide substrates and a microinjection assay to compare the structure function relationship of Ret/ptc2 mutants. A number of tyrosine residues within the Ret/ptc2 kinase domain as well as the RI alpha dimerization domain are critical for eliciting a mitogenic response
Relationship Between Scavenger Receptors and UPA:PAI-l and UPA Receptors in Breast Cancer ( Book )
3 editions published between 1997 and 1999 in English and held by 2 libraries worldwide
This proposal focuses on the question whether a derangement in expression or trafficking of scavenger receptors, LRP and megalin, might alter their interaction with uPAR, uPA, and PAI-1 in breast cancer cells. We established cell systems and prepared several essential reagents (antibodies) to evaluate the levels of scavenger receptor expression in three estrogen-insensitive and one sensitive breast cell lines, one normal mammary cell line, and a human fibrosarcoma cell line. Our novel findings demonstrate: 1) Differences in the level of expression and distribution of scavenger receptors, uPAR, uPA, and PAI-1 in normal vs. tumor cells. The increased expression of uPAR and uPA in cancer cells mimics the situation in vivo in breast carcinomas where overexpression of uPAR and uPA is associated with poor prognosis; 2) Surface uPAR and LRP colocalize into a molecular complex and are co-internalized via clathrin-mediated endocytosis; 3) Direct interaction between the two receptors stabilizes these complexes even after dissociation of uPA:PAl-i in early endosomes; 4) LRP-dependent clearance of uPA:PAI-1-i from the cell surface also requires uPAR binding to LRP. These results document functional interactions between scavenger receptors (LRP) and uPAR suggesting a role for LRP in the regulation of plasminogen activation in cancer cells
Vascular Endothelial Growth Factor and Receptors in Breast Cancer ( Book )
4 editions published between 1997 and 1998 in English and held by 2 libraries worldwide
Experiments completed in the past year were designed to characterize the modifications in mammary gland and tumor vascular endothelium. Studies included analysis of VEGF isoform expression and vascular morphology in mammary gland tumors, mammary glands from ovariectomized (implanted with estrogen or placebo pellets), pregnant, lactating and involuting mice. Our findings demonstrate that estrogen alone can up-regulate the expression of VEGF 120, 164, and 188 isoforms and induce continuous vascular endothelium to become fenestrated. In all cases, where estrogen was present, VEGF was up- regulated, vessels were proliferating, vascular endothelium was fenestrated and caveolae were clustered and fused. Moreover in situations, where estrogen was lacking or decreasing, vascular endothelium had no fenestrations or clustered, fused caveolae. It is known that microvascular permeability is increased with the presence of fenestrae and clustered, fused caveolae
Remote Sensing of Inner Heliospheric Plasmas ( Book )
5 editions published between 1991 and 1997 in English and held by 2 libraries worldwide
Solar disturbances produce major effects on the corona, the solar wind, the interplanetary medium, and the Earth along with its magnetosphere. We have developed new techniques for studying plasma disturbances in the inner heliosphere by remotely sensing them. These techniques use data from the HELIOS spacecraft zodiacal light photometers, the ISEE-3 spacecraft kilometer radio- wave experiment, and a variety of other spacecraft and ground-based instruments. The zodiacal light photometers on board the two HELIOS spacecraft (data coverage from 1974 to 1986) provide the first good information about the heliospheric masses and shapes of propagating disturbances. Metric and kilometric type II and type III radiation caused by shock waves and fast moving electrons respectively are another way to remotely sense the structures which propagate outward from the Sun. The best kilometric radio wave sensing of inner heliospheric plasma is available from the ISEE-3 spacecraft. The investigations into the physics of the disturbances sensed by these techniques and the ability to forecast their occurrences are well underway
The Primary Sequence of Acetylcholinesterase and Selective Antibodies for the Detection of Organophosphate Toxicity ( Book )
3 editions published between 1987 and 1991 in English and held by 2 libraries worldwide
The proposed work has been directed to determination of the structure of acetylcholinesterase. During the project period we have determined the primary structure of the Torpedo enzyme through amino acid sequencing and the isolation and sequencing of a cDNA clone encoding for the 11s form of the enzyme. The recombinant DNA studies involving the isolation of cDNA clones encoding acetylcholinesterase were part of studies supported by an NIH grant. However, they relied on amino acid sequence information for preparation of oligonucleotides for screening and ultimately yielded complementary information on primary structure. Peptides corresponding to the active center of the enzyme and a C-terminal region have been synthesized and antibodies are being raised for the purpose of detecting the phosphorylated enzyme and delineating functional regions of the molecule. Finally, the disulfide bonding pattern has been determined for the 11s form of the enzyme. Keywords: Molecular structure; Antibodies; (kt)
VR/Multimedia Synthesis, Phase II (with) Appendix 8 and Appendix 9 (Videotape) ( Book )
3 editions published between 1997 and 2000 in English and held by 1 library worldwide
ELECTRONIC FILE CHARACTERISTICS: VHS-format video. PHYSICAL DESCRIPTION: 2 videotapes; 1/2 in. ea.; 35 mins. and 6.45 mins. SYSTEMS DETAIL NOTE: Requires VHS video player and monitor. ABSTRACT: The Learning Resources Center (LRC), the hub of technology-based educational research at the University of California, San Diego (UCSD) School Of Medicine, has a strong track record for developing and distributing cutting-edge curricular resources. Under grants from DARPA (N000l4-93-l-l278 and DAMD l7-94-j-4487) and ONR (N00014-97-l-0356), the LRC has spent five years developing "VisualizeR (C)", a virtual environment for the teaching and learning of complex three-dimensional (3D) structures in conjunction with large quantities of diverse pedagogic materials. "Anatomic VisualizeR (C)" combines 3D models (based on the National Library of Medicine's Visible Human (TM) Dataset) with supporting 2D media (e.g., diagnostic imagery, surgical videos) to create compelling lessons in anatomy. The multimodal approach ("Virtual Reality - Multimedia Synthesis") is realized as a virtual dissection room where students can directly interact with 3D models and concurrently access supporting curricular materials. Anatomic Visualize (R) learning modules (e.g., skull, ear, abdomen, and skeleton) have been piloted with high school students at Southwest High School (a health sciences magnet school in San Diego county), medical students at UCSD, and graduate nursing students at the Unformed Services University of the Health Sciences in Bethesda, Maryland
Estrogen Nuclear Receptor Coactivators in Pathogenesis of Breast Cancer ( Book )
3 editions published between 1998 and 1999 in English and held by 1 library worldwide
This project focuses on the role of nuclear receptor coactivators in steroid dependent regulation of cell growth in pathogenesis of breast cancer. A novel nuclear receptor coactivator of transcription, p300 and CBP associated factor (p/CAF) has been shown to be required for estrogen, thyroid hormone, and retinoic acid-dependent gene expression. The histone acetyltransferase activity of p/CAF was demonstrated to play essential role in nuclear receptor dependent gene expression and it appeared to be transcription factor specific. The ligand-dependent association of p/CAF with nuclear receptor depends upon nuclear receptor corepressor (NCoR) dismissal. These data suggest that p/CAF complex plays a central role in nuclear receptor dependent gene regulation, a key element in steroid dependent cancer development
Feasibility of Dual Optics/Ultrasound Imaging and Contrast Media for the Detection and Characterization of Prostate Cancer ( Book )
3 editions published between 2008 and 2010 in English and held by 1 library worldwide
This research project focuses on prostate cancer, a devastating socioeconomic disease, whose detection is plagued with inadequate sensitivity and specificity. Hypoxia is the hallmark of malignancy because aggressive cancers outgrow their blood supply. We ultimately aim to build an instrument that combines OPtics and UltraSound (OPUS) to quantify hypoxia via optical imaging but with the improved spatial resolution of US imaging. Specifically, the acousto-optic effect will be used to only modulate light (at the ultrasound frequency) which propagates through a small ultrasound focal zone. This DOD Idea Development Award is concerned with the development of a novel acousto-optic detection idea based on quadrature measurements with a gain-modulated image intensified CCD camera. Furthermore, we proposed the novel idea of using microbubble-based contrast agents to significantly increase the light modulation and, moreover, the use of fluorescent microbubbles to provide additional enhancement. During the first year of the research project we have demonstrated the detection of ultrasound-modulated incoherent photons followed by the novel quadrature detection of ultrasound-modulated photons and fluorescence photons with the gain-modulated image intensified CCD camera approach. This research demonstrates the potential to perform acousto-optic molecular imaging of prostate cancer with incoherent and fluorescence photons using endogenous contrast, e.g. hypoxia, and also fluorescent probes
Molecular Mechanisms of Glial Abnormalities in Neurofibromatosis ( Book )
3 editions published between 1998 and 2000 in English and held by 1 library worldwide
Neurofibromatosis is a genetic disease in which the major pathology is due to abnormal proliferation of Schwann cells. One of the two tumor suppressor genes mutated in this disease is the neurofibromatosis 1 gene (NF1). The product of this gene, neurofibromin, inhibits ras function. Based on several unique features of control of growth in Schwann cells, we believe that specific factors associated with CREB, and the CREB-binding protein, CBP, serve to specifically modulate cAMP and ras -dependent growth events in glia, and underly the NF1 mutation-induced Schwann cell proliferation. Our identification of CBP as an integral component of the activation complex for cAMP and Growth factors, and evidence that competition for limiting amounts of CBP can result in AP-1 inhibiting events, has led us to provide evidence for a molecular mechanism which integrates diverse signaling pathways, dictating differentiation and proliferation events. We hypothesize that this regulatory system can be exploited to discover novel approaches to the treatment of neurofibromatosis. We have proposed to generate various genetic models exhibiting altered cAMP and ras -dependent signaling pathways and utilize these model systems to identify and characterize factors which are responsible for the etiology of neurofibromatosis type 1
Cholinesterase Structure: Identification of Residues and Domains Affecting Organophosphate Inhibition and Catalysis ( Book )
5 editions published between 1996 and 1999 in English and held by 1 library worldwide
During the project period we have initiated a completed studies in the following areas: (1) The crystal structure of a mouse acetylcholinesterase-fasciculin 2 complex has provided an essential template for structure-function studies; progress has been made in crystallizing acetylcholinesterasein the absence of fasciculin. (2) Studies of a series of enantiomeric organophosphates reacting with acetylcholinesterase have been completed; they have yielded vital information on their binding orientation in the ground and transition states. Residues on the enzyme governing enantiomer specificity and leaving group orientation have been defined through site-specific mutagenesis. (3) The interactions of fasciculin 2 with acetylcholinesterase have been studied by kinetic and site-specific mutagenesis methods. The fasciculin 2-acetylcholinesterase complex has enabled us to study entry of ligands to the active center gorge. (4) Studies in oxime reactivation of cholinesterase inhibited by the enantiomeric phosphates were undeataken using 2-PAM and HI-6 with wild-type and mutant acetylcholinesterases
Design and Packaging of Fault Tolerant Optoelectronic Multiprocessor Computing Systems ( file )
4 editions published between 1991 and 1993 in English and held by 0 libraries worldwide
There is considerable interest in developing optical interconnects for multi-chip modules (MCM). As a consequence, there is the basic need in developing a methodology for partitioning the system for effective utilization of the optical and electronic technologies. For the given netlist of a system design, key question to be answered is where to use optical interconnections. This paper introduces the Computer Aided Design (CAD) approach for partitioning opto-electronic systems into Opto-Electronic Multichip Modules (OE MCM). We will first discuss the design tradeoff issues in optoelectronic system design including speed, power dissipation, area and diffraction limits for free space optics. We will then define a formulation for OE MCM partitioning and describe new algorithms for optimizing this partitioning based on the minimization of the power dissipation. The models for the algorithms are discussed in detail and an example of a multistage interconnect network is given. Different results, with the number and size of chips being variable, are presented where improvement for the system packaging has been observed when the partitioning algorithm are applied
Cholinesterase Structure: Identification of Mechanisms and Residues Involved in Organophosphate Inhibition and Enzyme Reactivation ( file )
3 editions published between 2003 and 2005 in English and held by 0 libraries worldwide
Studies on the structural of acetylcholinesterase (AChE) as a target of organophosphate toxicity continue and have yielded several leads of significance and practical outcomes. First, studies on oxime reactivation reveal the importance of achieving a suitable angle of attack for the oxime within the confines of the active center gorge. Through the use of mutant AChE-oxime combinations, oxime-assisted catalytic turnover of organophosphates can be achieved such that mutant AChE can be employed with oximes as a catalytic scavenger. Second, through cysteine substitution mutagenesis and acrylodan labeling we have developed a fluorescent enzyme whose emission spectrum changes upon conjugation with organophosphate. These enzymes are now being immobilized and developed as remote sensors for AChE inhibition. Third, we have developed mass spectrometry methods to detect directly the organophosphate conjugates with AChE. Lastly, we have developed several transgenic (knock-out) animal strains that enable us to study the roles cholinesterase inhibition centrally and in the periphery play in organophosphate toxicity and whether the antidotal actions of oximes arise solely through reactivation
Fundamentals of Acoustic Instabilities in Liquid Propellant Rockets ( file )
3 editions published between 1992 and 1999 in English and held by 0 libraries worldwide
The objective of this research was to improve understanding of the mechanisms by which flow, mixing and combustion processes are coupled to acoustic fields in liquid propellant rocket motors. Particular attention was focused on analyses of amplification mechanisms coupled with finite rate chemical reactions by use of numerical and analytical methods. A theoretical explanation of empirical correlation of instability boundaries for engine test results for LOX/RP-1 rockets was developed on the basis of amplification by finite rate chemical reactions in strained mixing layers. In addition, a new numerical computation of nonlinear amplification mechanisms in LOX/GH2 combustion suggested a possible explanation of threshold phenomena found in liquid propellant rockets
Mechanism of Action of Substituted Indanones in Multidrug Resistant Breast Cancer ( file )
3 editions published between 2000 and 2002 in English and held by 0 libraries worldwide
Our laboratory has recently synthesized a series of novel substituted indanones that are selectively toxic to multidrug resistant cancer cells, including breast cancer cell lines. In this application we proposed to characterize the mechanism of action of indanocine and to assess the in vivo anti-tumor activity of indanocine. During the second year we: - published the second report on the biological activity of indanocine (Cancer Res 2001 Oct 1;61(19):7248.54) - analyzed the indanocine-resistant stable cell line - identified the potential indanocine-binding site on tubulin - continued the animal testing of indanocine - studies the pro-apoptotic mechanism of action in non-dividing tumor cells The results shown in this annual summary demonstrate that indanocine is a very promising new anti-cancer agent, with selective activity in slowly-dividing or quiescent tumor cells. The positive early animal models suggest that indanocine could be soon ready for clinical trials
Theories of Turbulent Combustion in High Speed Flows ( file )
5 editions published between 1991 and 1995 in English and held by 0 libraries worldwide
This research involves theoretical studies of the chemical and fluid mechanical phenomena which make turbulent combustion in high-speed flows different from such combustion in low-speed flows. Finite-rate chemistry plays a significant role in high-speed flows because of the small ratios of flow times to chemical times. The studies address ignition and extinction phenomena in nonpremixed turbulent combustion of hydrogen-air systems by both numerical and asymptotic methods. Attention also is paid to effects of compressibility in high-speed turbulent combustion, with consideration given to interdispersal configurations of shocklets and flamelets. Efforts are made to provide a firmer foundation for the modeling of high-speed turbulent reacting flows, to aid in the development of a formulation which gives results that can be compared with experiments, on turbulent combustion ... Turbulent flames, Diffusion flames, Supersonic combustion
Nonlinear Spatio-Temporal Processing of Femtosecond Pulses for Ultrahigh Bandwidth Communication ( file )
2 editions published between 1999 and 2000 in English and held by 0 libraries worldwide
We emphasize developing high speed and high efficiency parallel to serial multiplexers and serial to parallel demultiplexers, the two most critical challenges for current technology to meet the needs of ultra high bandwidth communication applications. In the following we describe research on nonlinear spatio temporal processors using cascaded second order nonlinearity to perform (1) Spatial temporal wave mixing for space to time conversion, and (2) Instantaneous time reversal and phase conjugation of ultrafast waveforms. In addition, we will also report our study on a (3) Femtosecond single shot pulse imaging system that uses transverse time delay pulse cross correlation
Skeletal Muscle Ischemia and Heat Shock Proteins ( file )
3 editions published between 1994 and 1996 in English and held by 0 libraries worldwide
Blood loss testing in decreased organ perfusion and subsequent ischemic injury of cardiac and skeletal muscle presents a significant problem for the soldier in combat. Recent findings indicate that different forms of noxious stress including exposure to increased temperature, noxious chemical agents, and schemia lead to increased expression of heat shock proteins (hsp) which have a protective effect against injury induced by noxious stimuli. We wanted to determine in this proposal if a muscle derived permanent cell line expressing increased amounts of hsp7O will show protection against damage induced by simulated ischemia. To generate cell lines which permanently overexpress the inducible hsp7O (hsp7Oi) proteins, cells will be transfected with a neomycin resistance gene and the hsp7Oi gene. Stable lines will be selected by growing L6 cells in the presence of neomycin. Cells which have the neomycin resistance gene and the hsp7O gene incorporated into their DNA will survive. Such stably transfected cell lines will then be exposed to simulated ischemia consisting of hypoxia, absence of glucose, low tonicity, and resultant schemic damage will be determined by quantitating cell viability measured in colony formation assays, the inhibition of protein synthesis, and the release of cytoplasmic enzymes like creatine kinase. These studies will determine if hsp7Oi exerts a protective effect against ischemia mediated muscle injury. Demonstrating a protective effect of hsp7O protein will make it a useful agent to reduce ischemic muscle damage in soldiers exposed to muscle injury in combat
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