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Wysocki, Lawrence J.

Overview
Works: 2 works in 3 publications in 1 language and 3 library holdings
Publication Timeline
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Publications about Lawrence J Wysocki
Publications by Lawrence J Wysocki
Most widely held works by Lawrence J Wysocki
Dissecting the Immunogenicity of Monoclonal Antibodies ( file )
1 edition published in 2001 in English and held by 0 libraries worldwide
The potential of mononclonal antibodies, (mAbs), for use in therapeutic and diagnostic applications has not been fully realized in part due to counter-immune responses that often arise in patient recipients of mAb. A growing research effort to humanize mAb has focused primarily on the structure or sequence of the antibody variable (V) region domains. However, these approaches may ultimately suffer, as they overlook the requirement of T cell help for the immune counter-reaction and the potential of somatic hypermutation and V-D-J recombination to generate target T cell epitopes within mAb V regions. My approach focuses on this issue. In order to understand some basic principals concerning anti-immunoglobulin immune responses, I have developed methods, reagents and mouse strains during the previous funding year to effectively study T cell epitopes in mAb V regions. I was able to reproducibly distinguish an immune response to a V-region T cell epitope via a lymph node proliferation assay. Furthermore, I have generated MHC-tetrameric staining reagents with which I can identify T cells reactive with two such epitopes. Finally, I have generated congenic mouse strains containing or lacking the enzyme terminal deoxynucleotidyl transferase, (TdT), in order to address the role of T cell epitopes in junctional diversity
Dissecting Immunogenicity of Monoclonal Antibodies ( file )
2 editions published between 2002 and 2003 in English and held by 0 libraries worldwide
The potential of monoclonal antibodies, (mAbs), for use in therapeutic and diagnostic applications has not been fully realized in part due to counter-immune responses that often arise in patient recipients of mAb. A growing research effort to "humanize" mAb has focused primarily on the structure or sequence of the antibody variable (V) region domains. However, these approaches may ultimately suffer, as they overlook the requirement of T cell help for the immune counter-reaction and the potential of somatic hypermutation and V-D-J recombination to generate target T cell epitopes within mAb V regions. My approach focuses on this issue. In order to understand some basic principals concerning anti-immunoglobulin immune responses, I have developed a panal of T cell hybridomas and new transgenic mice. Studies with these tools strongly support our basic hypothesis that T cells are tolerant of endogenous immunoglobulin-derived diversity
 
Languages
English (3)
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