WorldCat Identities

Murray, Graeme I.

Overview
Works: 17 works in 68 publications in 1 language and 868 library holdings
Genres: Laboratory manuals  Academic theses 
Roles: Editor, Author, htt, Other, Contributor
Classifications: QH506, 572.8028
Publication Timeline
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Most widely held works by Graeme I Murray
Laser capture microdissection : methods and protocols by Graeme I Murray( )

30 editions published between 2004 and 2018 in English and held by 663 WorldCat member libraries worldwide

Describes in step-by-step detail how to successfully carry out tissue-based laser microdissection in the laboratory using the different systems available and to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. Highlights include gene expression profiling of primary tumor cell populations using laser capture microdissection, RNA transcript amplification and GeneChip microarrays, laser capture microdissection and colorectal cancer proteomics, and proteomic analysis of human bladder tissue using SELDI approach following microdissection techniques. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics
Cytochromes P450 Role in the metabolism and toxicity of drugs and other xenobiotics( )

1 edition published in 2008 in English and held by 47 WorldCat member libraries worldwide

With contributions by a team of internationally respected scientists, this book provides up-to-date information on the extensively studied cytochrome P450 enzyme in a very accessible manner, During half a century, cytochrome P450 in its original uniqueness as an optically "wrong" cytochrome has attracted many investigators, who have contributed to the unveiling of a bewildering multiplicity of biologically important functions of the, by now very large, superfamily of cytochrome P 450 enzymes. With its discovery in 1958 and with the advent of more refined spectroscopic methodologies, through the double wavelength spectrophotometry, the mysterious enzyme system began to reveal its secrets in a swift stream of investigative successes. As one of the most extensively studied enzyme systems worldwide the interest in cytochromes P450 very much reflects its importance in the elimination of drugs and other chemicals from the body and its role in chemical toxicity and in the aetiology of diseases such as cancer. There has been significant progress in research in this area in recent years and current books on this subject are now out of date. This much needed, new, fully up-to-date publication fills this gap and emphasises the new relevant topics that have emerged during the last decade in an easily accessible manner. The enzyme system, cytochromes P450, comprises a number of families/subfamilies, and the focus of the book is to deal with each individually, furnishing information directly relevant to scientists involved in the development of chemicals, in particular in the evaluation of their safety. The book has contributions from internationally respected scientists who are research-active in the relevant areas. The authors have made extensive use of figures and tables so that the reader can access the necessary information without always having to read the text. In addition, a very extensive, user-friendly index is a unique hallmark of the book. Part A of this monograph introduces the reader to the current knowledge of the evolutionary development of cytochrome P450 structure and function. Furthermore, it deals with the role of this enzyme in the formation of reactive intermediates. The shrewd and extensive utilisation of the molecular biology methodology very rapidly led to a vast body of enzymes calling for a classification of the plethora of different cytochromes P450 (the superfamily) into families and subfamilies. This is aptly exemplified by the ten chapters in Part B of this book, dealing with ten subfamilies and two families of cytochrome P450. Part C offers an insight into another aspect of cytochrome P450 research, namely its regulation through receptor-mediated stimuli - as opposed to enzyme induction or inhibition. The final chapter translates the current data on one of several drug metabolising systems into clinical application and highlights the role of cytochromes P450 in the treatment of neoplastic growth. The book deals extensively with each family/subfamily of the cytochromes P450 that contribute to the metabolism of xenobiotics. Essential and invaluable information is provided for the industrial research scientist working with fine chemicals, and especially those in the pharmaceutical industry, dealing with the safety evaluation of chemicals or being involved in the study of their metabolism, pharmacokinetics and toxicological properties. It should also prove of interest to Regulators concerned with the safety evaluation of chemicals, research pharmacologists and toxicologists, and postgraduate students studying drug metabolism and toxicology at an advanced level
Laser Capture Microdissection Methods and Protocols by Graeme I Murray( )

9 editions published in 2011 in English and held by 37 WorldCat member libraries worldwide

Laser microdissection techniques have revolutionized the ability of researchers in general, and pathologists in particular, to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies including PCR, microarrays, and proteomics. In second edition of Laser Capture Microdissection: Methods and Protocols, experts in the field provide the reader with practical advice on how to carry out tissue-based laser microdissection successfully in their own laboratory using the different laser microdissection systems that are available and to apply a wide range of molecular technologies. The individual chapters encompass detailed descriptions of the individual laser based micro-dissection systems. The downstream applications of the laser microdissected tissue described in the book include PCR in its many different forms as well as gene expression analysis including application to microarrays and proteomics. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Laser Capture Microdissection: Methods and Protocols, Second Edition is an ideal resource for researchers striving to move forward our understanding of normal physiology and pathology
Cytochromes P450 by D. F. V Lewis( Book )

2 editions published in 2008 in English and held by 36 WorldCat member libraries worldwide

With contributions by a team of internationally respected scientists, this book provides up-to-date information on the extensively studied cytochrome P450 enzyme in a very accessible manner
Cytochromes P450 : role in the metabolism and toxicity of drugs and other xenobiotics by Costas Ioannides( )

3 editions published in 2008 in English and held by 35 WorldCat member libraries worldwide

With contributions by a team of internationally respected scientists, this book provides up-to-date information on the extensively studied cytochrome P450 enzyme in a very accessible manner
Laser capture microdissection : methods and protocols by Graeme I Murray( Book )

8 editions published in 2005 in English and held by 26 WorldCat member libraries worldwide

Use of laser microdissection has grown enormously to become a very powerful high-throughput technology essential to a range of downstream applications in pathology, cancer research, life sciences, medical diagnostics, and biotechnology. In Laser Capture Microdissection, expert laboratory researchers noted for their optimized methods describe in step-by-step detail not only how to successfully carry out tissue-based laser microdissection in their own laboratories using the different systems available, but also how to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. These readily reproducible methods make it possible to carry out contamination-free molecular analyses of specific types of normal and diseased cells. Highlights include gene expression profiling of primary tumor cell populations, RNA transcript amplification and GeneChip® microarrays, colorectal cancer proteomics, and proteomic analysis of human bladder tissue using the SELDI® approach. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics. The protocols follow the successful Methods in Molecular Biology"!series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Laser Capture Microdissection provides a highly practical guide to successful tissue-based laser microdissection using available commercial systems and to the interpretation of the results using a wide range of sophisticated molecular technologies
Cytochemical studies of drug metabolising enzymes by Graeme I Murray( Book )

3 editions published in 1989 in English and held by 5 WorldCat member libraries worldwide

Cytochemical studies have been performed to investigate several enzymes which are involved in the metabolism of drugs, toxins, carcinogens and a variety of endogenous substances. Three groups of enzymes were studied: cytochrome P-450, gamma-glutamyl transpeptidase and NADP linked dehydrogenases. Also studied was glutathione, a peptide closely associated with these enzymes. The distribution of cytochrome P-450 in human tissues was determined by immunocytochemistry with monoclonal antibodies to a major constitutive form of human hepatic cytochrome P-450. Within the liver, cytochrome P-450 was present predominantly in hepatocytes of zone 3 of the liver acinus. Cytochrome P-450 was also demonstrated in the columnar absorptive cells of small intestinal villi, polymorphonuclear leucocytes and their precursors and mast cells. A novel cytochemical technique for demonstrating glutathione was developed. Glutathione was identified using o-phthaldialdehyde to produce a fluorophore which was visualised with fluorescence microscopy. This method was used to study the localisation and distribution of glutathione in breast lesions, particularly invasive carcinomas, where a variable level of glutathione in different carcinomas was demonstrated. A method was devised for the enzyme histochemical demonstration of gamma-glutamyl transpeptidase in fixed resin embedded tissue. Enzyme activity was optimally maintained and accurately localised in tissue displaying excellent morphology by fixing tissue in cold methanol free formaldehyde and then embeddding the tissue at low temperature in glycol methacrylate resin. Several NADP linked dehydrogenases and NADPH-cytochrome P-450 reductase were localised by enzyme histochemistry in sections of freeze-dried resin embedded tissue. This novel method of tissue preparation was evolved to allow the demonstration of the activity of dehydrogenases in resin embedded tissue
Laser Capture Microdissection Methods and Protocols by Graeme I Murray( Book )

1 edition published in 2005 in English and held by 3 WorldCat member libraries worldwide

Use of laser microdissection has grown enormously to become a very powerful high-throughput technology essential to a range of downstream applications in pathology, cancer research, life sciences, medical diagnostics, and biotechnology. In Laser Capture Microdissection, expert laboratory researchers noted for their optimized methods describe in step-by-step detail not only how to successfully carry out tissue-based laser microdissection in their own laboratories using the different systems available, but also how to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. These readily reproducible methods make it possible to carry out contamination-free molecular analyses of specific types of normal and diseased cells. Highlights include gene expression profiling of primary tumor cell populations, RNA transcript amplification and GeneChip® microarrays, colorectal cancer proteomics, and proteomic analysis of human bladder tissue using the SELDI® approach. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Laser Capture Microdissection provides a highly practical guide to successful tissue-based laser microdissection using available commercial systems and to the interpretation of the results using a wide range of sophisticated molecular technologies
Extending colonic mucosal microbiome analysis--assessment of colonic lavage as a proxy for endoscopic colonic biopsies by Euan Watt( )

1 edition published in 2016 in English and held by 2 WorldCat member libraries worldwide

Laser Capture Microdissection Methods and Protocols by Graeme I Murray( )

1 edition published in 2005 in English and held by 2 WorldCat member libraries worldwide

Epidermal Growth Factor (EGFR) copy number aberrations in esophageal and gastro-esophageal junctional carcinoma by Åsa Dahle-Smith( )

1 edition published in 2015 in English and held by 2 WorldCat member libraries worldwide

Laser capture microdissection by Graeme I Murray( Book )

3 editions published between 2010 and 2018 in English and Undetermined and held by 2 WorldCat member libraries worldwide

Sensitivity of Colorectal Cancer to Arginine Deprivation Therapy is Shaped by Differential Expression of Urea Cycle Enzymes by Constantinos Alexandrou( )

1 edition published in 2018 in English and held by 2 WorldCat member libraries worldwide

APRIL is a novel clinical chemo-resistance biomarker in colorectal adenocarcinoma identified by gene expression profiling by Russell D Petty( )

1 edition published in 2009 in English and held by 2 WorldCat member libraries worldwide

<> by Sabelo Hadebe( )

1 edition published in 2016 in English and held by 2 WorldCat member libraries worldwide

Laser Capture Microdissection : Methods and Protocols by Graeme I Murray( )

1 edition published in 2005 in English and held by 1 WorldCat member library worldwide

Use of laser microdissection has grown enormously to become a very powerful high-throughput technology essential to a range of downstream applications in pathology, cancer research, life sciences, medical diagnostics, and biotechnology. In Laser Capture Microdissection, expert laboratory researchers noted for their optimized methods describe in step-by-step detail not only how to successfully carry out tissue-based laser microdissection in their own laboratories using the different systems available, but also how to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. These readily reproducible methods make it possible to carry out contamination-free molecular analyses of specific types of normal and diseased cells. Highlights include gene expression profiling of primary tumor cell populations, RNA transcript amplification and GeneChip® microarrays, colorectal cancer proteomics, and proteomic analysis of human bladder tissue using the SELDI® approach. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Laser Capture Microdissection provides a highly practical guide to successful tissue-based laser microdissection using available commercial systems and to the interpretation of the results using a wide range of sophisticated molecular technologies
CYP3A isoforms in Ewing's sarcoma tumours: an immunohistochemical study with clinical correlation( )

1 edition published in 2015 in English and held by 1 WorldCat member library worldwide

Ewing's sarcoma is an aggressive malignancy of bone and soft tissue with high incidence of metastasis and resistance to chemotherapy. Cytochrome P450 (CYP) monooxygenases are a family of enzymes that are involved in the metabolism of exogenous and endogenous compounds, including anti-cancer drugs, and have been implicated in the aggressive behaviour of various malignancies. Tumour samples and clinical information including age, sex, tumour site, tumour size, clinical stage and survival were collected from 36 adult and paediatric patients with Ewing's sarcoma family tumours. Tissue microarrays slides were processed for immunohistochemical labelling for CYP3A4, CYP3A5 and CYP3A7 using liver sections as positive control. The intensity of staining was scored as negative, low or high expression and was analysed statistically for any association with patients' clinical information. Four cases were later excluded due to inadequate viable tissue. CYP3A4 staining was present in 26 (81%) cases with high expression noted in 13 (40%) of 32 cases. High expression was significantly associated with distant metastases (P <0.05). CYP3A5 and CYP3A7 were expressed in 5 and 13 cases respectively (15.6%, 40.6%). There was no association between the expression of CYP3A isoforms and age, sex, tumour size, or location (pelvic or extra-pelvic). None of the biomarkers showed any correlation with overall or disease-free survival. In conclusion, expression of CYP3A isoforms is noted in Ewing's sarcoma tumours and high CYP3A4 expression may be associated with metastasis. Additional studies are needed to further investigate the role of CYP3A4 in the prognosis of these tumours
 
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Laser capture microdissection : methods and protocols Laser capture microdissection : methods and protocols
Covers
Cytochromes P450 Role in the metabolism and toxicity of drugs and other xenobioticsLaser Capture Microdissection Methods and ProtocolsCytochromes P450Cytochromes P450 : role in the metabolism and toxicity of drugs and other xenobioticsLaser capture microdissection : methods and protocolsLaser Capture Microdissection Methods and ProtocolsLaser Capture Microdissection Methods and ProtocolsLaser capture microdissectionLaser Capture Microdissection : Methods and Protocols
Alternative Names
Murray, Graeme

Languages
English (67)