WorldCat Identities

Hills, Gillian 1944-

Overview
Works: 81 works in 130 publications in 3 languages and 1,867 library holdings
Genres: Drama  Film adaptations  Thrillers (Motion pictures)  Detective and mystery films  Melodramas (Motion pictures)  Juvenile works  Horror films  Fiction  Crime films  Romance films 
Roles: Actor, Author, Singer, Lyricist, Composer, Editor, Performer
Classifications: PN1997, 791.4372
Publication Timeline
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Most widely held works by Gillian Hills
Blowup by Michelangelo Antonioni( Visual )

14 editions published between 1966 and 2017 in English and German and held by 1,153 WorldCat member libraries worldwide

Thomas' camera never flinches. Not at love without meaning. Not at the dazzle and madness of youth. Not at a murder revealed in a photographic Blow-Up. Fashion photographer Thomas (David Hemmings) casually takes a somewhat voyeuristic shot of a man and a young woman in each other's arms on a park bench. The young woman (Academy Award winner Vanessa Redgrave) follows Thomas home and makes love to him in exchange for the photograph. But Thomas keeps the negative, and when he enlarges it, what had seemed a carnal moment appears to be murder. Thomas returns to the park, and discovers that the man in the photograph is dead. Yet whenThomas enlarges the photo again, he notices a shadow in the bushes that could be barrel of the gun. Is the woman with whom Thomas made love a murderer? Reality seems to change with each Blow-Up
Blowup( Visual )

3 editions published in 2017 in English and held by 313 WorldCat member libraries worldwide

A London photographer takes some pictures of a couple in a park and discovers that he may have recorded evidence of a murder
Les liaisons dangereuses 1960 by Roger Vadim( Visual )

2 editions published in 2002 in French and held by 145 WorldCat member libraries worldwide

An update of the French epistolary novel "Les liaisons dangereuses", set amongst the upper classes in Paris in the late 1950s
Digby: the biggest dog in the world by Carolyn Bear( Book )

2 editions published in 1974 in English and held by 56 WorldCat member libraries worldwide

When Billy's dog accidentally drinks a secret solution that causes gigantic growth, the army sets out to destroy him
Beat girl( Visual )

13 editions published between 1960 and 2010 in English and held by 38 WorldCat member libraries worldwide

This is the British version of Rebel Without a Cause, set in the beatnik world where jaded restless kids rule the night in smokey coffe bars, at suicidal chickie runs and wild impromptu parties. This is newly mastered from the British "X" rated "Continential" version never before released in the U.S
Demons of the mind( Visual )

4 editions published between 1994 and 2002 in English and Undetermined and held by 20 WorldCat member libraries worldwide

"Convinced that his family is doomed by hereditary evil, Count Zorn holds his daughter Elizabeth and his son Emil prisoner in his castle"--Back of container
Blow up (v.f.)( Visual )

2 editions published in 2013 in French and held by 13 WorldCat member libraries worldwide

A London photographer takes some pictures of a couple in a park and discovers that he may have recorded evidence of a murder
Social sector rents in Scotland by Steve Wilcox( Book )

2 editions published in 2007 in English and held by 9 WorldCat member libraries worldwide

An analysis of rents in 2005/06 and a review of future policy options
Home based : a research report on the provision of dispersed hostels by Gillian Hills( Book )

1 edition published in 1988 in English and held by 9 WorldCat member libraries worldwide

Private renting in Scotland : context information paper by Gillian Hills( Book )

2 editions published between 1995 and 1999 in English and held by 8 WorldCat member libraries worldwide

Demographic trends in Scotland : context information paper by Gillian Hills( Book )

2 editions published in 2000 in English and held by 6 WorldCat member libraries worldwide

Musique pop de Paris( Recording )

1 edition published in 2012 in French and held by 6 WorldCat member libraries worldwide

This upbeat collection is an eclectic array of French pop music from the 1960s. It features gems from ye-ye rock, a style akin to American go-go music that combined early rock 'n' roll and Gallic elements
Private rented tenants in Scotland by Gillian Hills( Book )

1 edition published in 1996 in English and held by 5 WorldCat member libraries worldwide

Une petite tasse d'anxièté( Visual )

in Undetermined and held by 4 WorldCat member libraries worldwide

Zou bisou bisou : tout en Français : the yé-yé years 1960-1965 by Gillian Hills( Recording )

3 editions published in 2017 in Undetermined and French and held by 4 WorldCat member libraries worldwide

Orchids in line by Gillian Hills( Book )

2 editions published in 1975 in English and held by 4 WorldCat member libraries worldwide

Zou bisou bisou( Recording )

in Undetermined and held by 3 WorldCat member libraries worldwide

Technology development for the over-expression, purification and crystallisation of human membrane proteins by Gillian Hills( )

3 editions published between 2010 and 2011 in English and held by 3 WorldCat member libraries worldwide

Currently, the field of mammalian membrane protein structural biology is in its infancy. Existing technologies and experiences have shown that it is possible to obtain the structures of mammalian membrane proteins if sufficient work and thought has been invested. However, there is still an urgent need to develop new methodologies and approaches to improve all aspects of this important area of biological research. Here, a series of novel technologies for the overproduction, purification and crystallisation of human membrane proteins are described which have been tested with a representative member from each of the G-protein coupled receptor (adenosine 2a receptor (A2aR)) and membrane enzyme (sterol isomerase (SI)) superfamilies. The methylotrophic yeast Pichia pastoris is an excellent host cell for the overproduction of recombinant proteins including membrane proteins of mammalian origin. However, the commercially available expression vectors are far from what is required to maximise the production levels as well as simplify the detergent extraction and purification of human membrane proteins. Here, a series of related expression constructs were made that had different combinations of tags at both ends of the recombinant protein. The final optimised expression vectors had a C3 protease-iLOV-biotin acceptor-His10 (CLBH) tag fused to the C-terminus of the recombinant protein. The -CLBH vectors gave high level production of both test proteins (one N<sub>in</sub> - hSI; one N<sub>out</sub> - hA2aR) that could be rapidly purified to homogeneity using a generic protocol. The position of the His10 tag did not affect the expression level of the recombinant protein. In contrast, fusion of the biotin acceptor domain to the C-terminus of the recombinant protein increased its expression by a factor of between 2-4. The biotin acceptor domain could also be fully biotinylated in vitro using recombinantly expressed biotin ligase allowing purification/immobilisation of the target protein with streptavidin beads. Removal of the expression/purification tags from the recombinant proteins with C3 protease occurred more efficiently than when TEV protease was used. An optimised protocol was developed that gave maximal production of our target proteins in fermenter culture at an induction temperature of 22°C. Care was taken to find a methanol feed rate that gave the highest levels of protein production without causing the accumulation of excess methanol in the culture (which is known to be toxic to the yeast). Using this protocol it was possible to make both hSI and hA2aR with a production level>10 mg of recombinant proteinper litre of culture. As most MPs are colourless, target protein identification is usually performed by methods such as radioligand binding and/or Western blotting. However, these techniques can be time-consuming, use a lot of protein and do not give any information on the aggregation state of the protein in detergent solution. Previously, it has been shown that the processes of identifying and analysing membrane proteins in detergent solution can be accelerated by attaching green fluorescent protein to the C-terminus of the recombinant MP. Here, the potential of the recently described iLOV fluorescence tag for membrane protein applications was assessed. iLOV was shown to be an useful tool for optimising processes such as yeast clonal selection, protein production in fermenter culture, detergent and construct screening as well as tracking recombinant MPs throughthe purification process. Of note, the iLOV tag allowed a direct assessment of the stability and dispersity state of both target MPs in a range of detergents by fluorescence size exclusion chromatography (FSEC). Using this approach, it was shown that wild-type hA2aR solubilised using a combination of dodecyl-[beta]Dmaltoside (DDM) and cholesteryl-hemisuccinate (CHS) aggregated during purification on a Ni2+ column. Furthermore, it was shown that the hA2aR agonist conformationally-fixed mutant Rag23 is stable in DDM without any CHS present. Moreover, Rag23 was found to be monodisperse in a series of short-chain detergents (decyl-[beta]D-maltoside, nonyl-[beta]D-maltoside (NM)) and [beta]-octylglucoside) suggesting that this mutant is well-suited to structural studies. SI was remarkably robust in short chain detergents demonstrating a reasonable level of stability inthe short chain detergent NM. The FSEC experiments showed that wild-type SI has considerably higher intrinsic stability than native hA2aR suggesting that membrane enzymes will prove to be more amenable to structural analysis than GPCRs. Rag23 and SI were both purified to homogeneity in a simple four-step procedure: i) Ni² purification, ii) cleavage with C3 protease, iii) reverse Ni² purification and iv) gel-filtration chromatography. A buffer/salt screen was devised that allowedthose conditions where SI had maximal thermostability in detergent-solution to beidentified. SI was found to have greatest stability in sodium phosphate buffer at acidic pH. Using this information, it was possible to purify monodisperse SI in DM suggesting that this protein may make an excellent candidate for structuralstudies too. Crystallisation trials with SI were performed using the commercially available sparse matrix screen MemSys/MemStart. In addition, a lipidic-sponge phase sparse-matrix crystallisation screen that was developed in collaboration with Prof. Richard Neutze (University of Chalmers, Sweden) was tested using SI. Cholesterol could be incorporated into all of the sponges that make up the screen upto a concentration of 10%. (This is important as the activity of many mammalian membrane proteins is cholesterol-dependent). To date, no diffracting crystals of SI have been obtained with either the conventional or lipidic-spongephase crystallisation approaches. In short, a series of novel technologies/methodologies have been developed that will act as a platform for future efforts to solve the structures of a wide-range of human membrane proteins
Johnny Tomorrow by Carolyn Bear( Book )

1 edition published in 1976 in English and held by 3 WorldCat member libraries worldwide

Salut les idoles by Johnny Hallyday( Recording )

2 editions published in 1983 in French and held by 2 WorldCat member libraries worldwide

Les plus grands succès des années 60-70 sur 2 CD
 
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Audience level: 0.35 (from 0.25 for Digby: the ... to 0.99 for Une petite ...)

Blowup
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Alternative Names
GILLIAN HILLS

Gillian Hills angol énekesnő, filmszínésznő

Gillian Hills britische Schauspielerin, Sängerin und Illustratorin

Gillian Hills British actress and singer

Gillian Hills Brits actrice

Gillian Hills brittiläinen näyttelijä ja laulaja

Gillian Hills fransk sångare och skådespelare

Gillian Hills fransk sanger og skuespiller

Gillian Hills fransk songar og skodespelar

Gillian Hillsová britská zpěvačka a herečka

Джиллиан Хиллс

ジリアン・ヒルズ

Languages
English (47)

French (8)

German (2)