WorldCat Identities

Schekman, Randy W.

Overview
Works: 28 works in 39 publications in 1 language and 207 library holdings
Genres: Periodicals  Conference papers and proceedings  Filmed lectures  Nonfiction films 
Roles: Editor, Author
Publication Timeline
.
Most widely held works by Randy W Schekman
Endoplasmic reticulum : a subject collection from Cold Spring Harbor perspectives in biology( Book )

4 editions published in 2013 in English and held by 104 WorldCat member libraries worldwide

Abstracts of papers presented at the 1993 meeting on yeast cell biology : August 17-August 22, 1993( Book )

1 edition published in 1993 in English and held by 14 WorldCat member libraries worldwide

Abstracts of papers presented at the 1987 meeting on yeast cell biology : August 11-August 16, 1987( Book )

3 editions published in 1987 in English and held by 12 WorldCat member libraries worldwide

Annual review of cell and developmental biology( Book )

2 editions published in 2005 in English and held by 11 WorldCat member libraries worldwide

Annual review of cell and developmental biology( Book )

2 editions published in 2000 in English and held by 11 WorldCat member libraries worldwide

Annual review of cell and developmental biology( Book )

2 editions published between 2004 and 2005 in English and held by 10 WorldCat member libraries worldwide

Annual review of cell and developmental biology( Book )

1 edition published in 2002 in English and held by 8 WorldCat member libraries worldwide

Annual review of cell and developmental biology : volume 26, 2010( Book )

1 edition published in 2010 in English and held by 5 WorldCat member libraries worldwide

Resolution and reconstitution of a multienzyme DNA replication reaction by Randy W Schekman( )

4 editions published in 1975 in English and held by 4 WorldCat member libraries worldwide

Annual review of cell and developmental biology( Book )

1 edition published in 2008 in English and held by 4 WorldCat member libraries worldwide

Annual review of cell and developmental biology( Book )

1 edition published in 2013 in English and held by 4 WorldCat member libraries worldwide

Annual review of cell and developmental biology( Book )

1 edition published in 2001 in English and held by 2 WorldCat member libraries worldwide

Molecular mechanisms of protein sorting in the secretory pathway( Visual )

1 edition published in 2003 in English and held by 1 WorldCat member library worldwide

Annual review of cell biology( Book )

1 edition published in 1999 in English and held by 1 WorldCat member library worldwide

Annual review of cell and developmental biology( Book )

1 edition published in 2003 in English and held by 1 WorldCat member library worldwide

Annual review of cell and developmental biology( Book )

1 edition published in 2002 in English and held by 1 WorldCat member library worldwide

Annual Review of Cell and Developmental Biology 2008( Book )

1 edition published in 2008 in English and held by 1 WorldCat member library worldwide

Biogenesis and function of the autophagosome by Randy W Schekman( Visual )

1 edition published in 2013 in English and held by 1 WorldCat member library worldwide

(CIT): The NIH Cell Biology and Metabolism Program will host Randy Schekman, co-winner of the 2013 Nobel Prize for Physiology or Medicine. The pathway of autophagy has assumed an important position in the analysis of mammalian cellular response to stress, hypoxia and pathogen infection. Autophagosomes mature by growth and envelopment of cytosolic proteins and organelles that are trapped within the inner membrane of a two-membrane organelle. Trapped proteins are delivered by autophagosome fusion to the lysosome where protein and polysaccharide degradation permit amino acids and sugars to be recycled. A pre-autophagsosomal membrane matures by the addition of membrane material from various intracellular sources and the attachment of peripheral proteins that remain bound through a covalent lipidation reaction. The origin and the mechanism of generation of the pre-autophagic membrane are poorly understood. Published evidence suggests an origin of this membrane on the ER, on the mitochondrial surface or possibly the Golgi or plasma membrane. We have addressed these issues with the development and analysis of a cell-free reaction that reproduces the lipidation of a major peripheral autophagosomal protein, LC3. Mouse embryonic fibroblasts (MEFs) taken from a mouse strain deficient in Atg5 are unable to lipidate LC3 and other targets of lipidation involved in the autophagic process. A crude membrane fraction isolated from these MEFs was mixed with cytosol harvested from normal cells that were untreated or subjected to a stress regimen known to induce autophagy. On addition of ATP, incubation of the mixture resulted in the formation of lipidated endogenous LC3 or exogenous recombinant LC3. The reaction required both membranes and cytosol and was stimulated 2-5 fold when the cytosol was taken from stress-induced cells, but was inactive when cytosol was used from stressed atg5 mutant MEFs. Autophagosome maturation requires a class III PI-3 kinase (VPS34 homolog); LC3 lipidation in our cell-free reaction was inhibited by wortmannin and 3-methyladenine, known inhibitors of this kinase, and by the addition of a peptide containing a PI3P-bnding sequence, the FYVE domain. Using cell fractionation techniques including differential centrifugation and buoyant density sedimentation, we have identified an ERGIC fraction enriched in Sec22 and ERGIC-53 that has the highest specific activity for LC3 lipidation in comparison to other cellular membranes. Membranes isolated from cells depleted of the ERGIC by use of selective inhibitors of traffic did not retain lipidation activity, but the activity reappeared quickly when traffic and the ERGIC was restored by removal of the inhibitor. Other early markers of the autophagosome membrane, Atg14L and DFCP1, are recruited to the ERGIC in cultured cells and in our cell-free reaction. This approach may now be exploited to understand the molecular mechanism of growth, closure and cargo capture by the maturing autophagosomal membrane
Protein sorting in the secretory pathway : lessons from yeast applied to mammalian development by Randy W Schekman( Visual )

1 edition published in 2012 in English and held by 1 WorldCat member library worldwide

Prof Randy Schekman's laboratory developed genetic and biochemical approaches to dissect the process of protein secretion in the baker's yeast, S.cerevisiae. The genes and proteins his lab discovered in yeast were found to organize the secretory pathway in all eukaryotes. Part of the pathway has been dissected using biochemical reconstitution of the formation and fusion of transport vesicles that mediate protein traffic from the endoplasmic reticulum to the Golgi apparatus. His lab has probed the molecular mechanism of defects in protein sorting that lead to human diseases of development such as spina bifida and a disease of craniofacial morphology that results in a tissue-selective defect in the secretion of collagen. Recent results suggest a role for ubiquitin conjugation of a coat protein complex in the envelopment of vesicles that covey collagen from the endoplasmic reticulum
Annual Review of Cell and Developmental Biology 2007( Book )

1 edition published in 2005 in English and held by 1 WorldCat member library worldwide

 
moreShow More Titles
fewerShow Fewer Titles
Audience Level
0
Audience Level
1
  Kids General Special  
Audience level: 0.65 (from 0.52 for Annual rev ... to 1.00 for Wit & wisd ...)

Annual review of cell and developmental biology
Covers
Annual review of cell and developmental biologyAnnual review of cell and developmental biologyAnnual review of cell and developmental biologyAnnual review of cell and developmental biology : volume 26, 2010Annual review of cell and developmental biologyAnnual review of cell biologyAnnual Review of Cell and Developmental Biology 2008
Alternative Names
Randy Schekman Amerikaans biochemicus

Randy Schekman amerikanischer Biochemiker

Randy Schekman biologiste cellulaire américain

Randy Schekman biólogo celular e investigador científico estadounidense

Randy Schekman biologo statunitense

Randy W. Schekman

Randy W. Schekman dokter asal Amerika Serikat

Randy W. Schekman nobelprisvinner i medisin 2013

Randy Wayne Schekman

Schekman, Randy Wayne

Ранди Шекман

Ренді Шекман

Рэндзі Шэкман

Рэнди Шекман

Шекман, Рэнди

רנדי שקמן

راندي شيكمان

رندی شکمن

رینڈی شیک مین

رینڈی شیکمان

रैंडी शेकमैन

র‍্যান্ডি ওয়েন শেকম্যান

ரேன்டி சேக்மன்

റാൻഡി ഡബ്ല്യൂ ഷെക്ക്മാൻ

රැන්ඩි ෂෙක්මන්

랜디 쉐크맨

ランディ・シェクマン

兰迪·谢克曼

Languages
English (39)