WorldCat Identities

Radlwimmer, Bernhard

Overview
Works: 10 works in 10 publications in 2 languages and 22 library holdings
Roles: Contributor, Other
Publication Timeline
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Most widely held works by Bernhard Radlwimmer
Co-localization of CENP-C and CENP-H to discontinuous domains of CENP-A chromatin at human neocentromeres by Alicia Alonso( )

1 edition published in 2007 in English and held by 2 WorldCat member libraries worldwide

Genomic and transcriptomic changes complement each other in the pathogenesis of sporadic Burkitt lymphoma by ICGC MMML-Seq Consortium( )

1 edition published in 2019 in English and held by 2 WorldCat member libraries worldwide

Array-CGH in unclear syndromic nephropathies identifies a microdeletion in Xq22.3-q23 by Alexander Hoischen( )

1 edition published in 2009 in English and held by 2 WorldCat member libraries worldwide

<> by Simon Raffel( )

1 edition published in 2018 in English and held by 2 WorldCat member libraries worldwide

Expansive growth of two glioblastoma stem-like cell lines is mediated by bFGF and not by EGF( )

1 edition published in 2013 in English and held by 2 WorldCat member libraries worldwide

Background. Patient-derived glioblastoma (GBM) stem-like cells (GSCs) represent a valuable model for basic and therapeutic research. GSCs are usually propagated in serum-free Neural Basal medium supplemented with bFGF and EGF. Yet, the exact influence of these growth factors on GSCs is still unclear. Recently it was suggested that GBM stemlike cells with amplified EGFR should be cultured in stem cell medium without EGF, as the presence of EGF induced rapid loss of EGFR amplification. However, patient biopsies are usually taken into culture before their genomic profiles are defined. Thus, an important question remains whether GBM cells without EGFR amplification also can be cultured in stem cell medium without EGF.Meterials and methods. To address this question, we used two heterogeneous glioblastoma GSC lines (NCH421k and NCH644) that lack EGFR amplification.Results. Although both cell lines showed very low EGFR expression under standard growth conditions, bFGF stimulation induced higher expression of EGFR in NCH644. In both cell lines, expression of the stem cell markers nestin and CD133 was higher upon stimulation with bFGF compared to EGF. Importantly, bFGF stimulated the growth of both cell lines, whereas EGF had no effect. We verified that the growth stimulation by bFGF was either mediated by proliferation (NCH421k) or resistance to apoptosis (NCH644).Conclusions. We demonstrate that GSC cultures without EGFR amplification can be maintained and expanded with bFGF, while the addition of EGF has no significant effect and therefore can be omitted
Matrix-comparative genomic hybridization from multicenter formalin-fixed paraffin-embedded colorectal cancer tissue blocks by the EORTC Gastrointestinal (GI) Group( )

1 edition published in 2007 in English and held by 2 WorldCat member libraries worldwide

Primary glioblastoma cultures: can profiling of stem cell markers predict radiotherapy sensitivity?( )

1 edition published in 2014 in English and held by 1 WorldCat member library worldwide

Abstract Human glioblastomas may be hierarchically organized. Within this hierarchy, glioblastoma-initiating cells have been proposed to be more resistant to radiochemotherapy and responsible for recurrence. Here, established stem cell markers and stem cell attributed characteristics such as self-renewal capacity and tumorigenicity have been profiled in primary glioblastoma cultures to predict radiosensitivity. Furthermore, the sensitivity to radiotherapy of different subpopulations within a single primary glioblastoma culture was analyzed by a flow cytometric approach using Nestin, SRY (sex-determining region Y)-box 2 (SOX2) and glial fibrillary acidic protein. The protein expression of Nestin and SOX2 as well as the mRNA levels of Musashi1, L1 cell adhesion molecule, CD133, Nestin, and pleiomorphic adenoma gene-like 2 inversely correlated with radioresistance in regard to the clonogenic potential. Only CD44 protein expression correlated positively with radioresistance. In terms of proliferation, Nestin protein expression and Musashi1, pleiomorphic adenoma gene-like 2, and CD133 mRNA levels are inversely correlated with radioresistance. Higher expression of stem cell markers does not correlate with resistance to radiochemotherapy in the cancer genome atlas glioblastoma collective. SOX2 expressing subpopulations exist within single primary glioblastoma cultures. These subpopulations predominantly form the proliferative pool of the primary cultures and are sensitive to irradiation. Thus, profiling of established stem cell markers revealed a surprising result. Except CD44, the tested stem cell markers showed an inverse correlation between expression and radioresistance. Markers used to define glioma-initiating cells (GIC) are generally not defining a more resistant, but rather a more sensitive group of glioma cells. An exemption is CD44 expression. Also proliferation of the GIC culture itself was not systematically associated with radiosensitivity or - resistance, but a SOX-2 positive, proliferative subgroup within a GIC culture is showing the highest radiosensitivity
Decreased expression of the mitochondrial BCAT protein correlates with improved patient survival in IDH-WT gliomas( )

1 edition published in 2016 in English and held by 1 WorldCat member library worldwide

LGR5 is a Marker of Poor Prognosis in Glioblastoma and is Required for Survival of Brain Cancer Stem-Like Cells( )

1 edition published in 2012 in English and held by 1 WorldCat member library worldwide

Abstract In various types of cancers including glioblastoma, accumulating evidence show the existence of cancer stem-like cells (CSCs), characterized by stem cell marker expression, capability of differentiation and self-renewal, and high potential for tumor propagation in vivo. LGR5, whose expression is positively regulated by the Wnt signaling pathway, is a stem cell marker in intestinal mucosa and hair follicle in the skin. As Wnt signaling is also involved in brain development, the function of LGR5 in the maintenance of brain CSCs is to be assessed. Our study showed that the LGR5 transcript level was increased in CSCs. Co-immunofluorescence staining demonstrated the co-localization of CD133- and LGR5-positive cells in glioblastoma tissue sections. Functionally, silencing of LGR5 by lentiviral shRNA-mediated knockdown induced apoptosis in brain CSCs. Moreover, LGR5 depletion led to a downregulation of L1 cell adhesion molecule expression. In line with an important function in glioma tumorigenesis, LGR5 expression increased with glioma progression and correlated with an adverse outcome. Our findings suggest that LGR5 plays a role in maintenance and/or survival of brain CSCs
 
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Audience level: 0.92 (from 0.84 for Expansive ... to 0.97 for Schlussber ...)

Alternative Names
Bernhard Radlwimmer onderzoeker

Bernhard Radlwimmer researcher

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