WorldCat Identities

Brault, Véronique

Overview
Works: 20 works in 22 publications in 2 languages and 27 library holdings
Roles: Thesis advisor, Other, Author, Opponent
Publication Timeline
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Most widely held works by Véronique Brault
Rôle des modifications posttraductionnelles des particules virales du CABYV dans la transmission par puceron by Sébastien Revollon( Book )

2 editions published in 2010 in French and held by 2 WorldCat member libraries worldwide

Des études antérieures réalisées sur un polérovirus, le Turnip yellows virus (TuYV), ont montré que la déglycosylation des particules virales du TuYV inhibait la transmission du virus par puceron, suggérant ainsi que les particules virales du TuYV pouvaient être glycosylées et que la glycosylation pouvait jouer un rôle crucial dans la vection de ce virus. La première partie de ce mémoire a consisté à déterminer la présence de glycosylation sur les particules virus du Cucurbit aphid-borne yellow virus (CABYV). Différentes approches ont été mises en oeuvres pour déterminer la présence de résidus glycosylées : mutation ponctuelle des sites potentiel de N-glycosylation, immunodétection des glycanes et spectrométrie de masse. Toutes ces approches vont dans le sens d'une absence de glycosylation des protéines structurales du CABYV. No us avons cependant mis en évidence qu'une protéine de plante copurifiant avec le virus était glycosylée, permettant d'expliquer les résultats obtenus précédemment. La deuxième partie de ce mémoire décrit l'étude du rôle de différentes protéines de plantes dans le mécanisme de transmission par puceron des polérovirus. Un effet stimulateur sur la transmission est observé, cependant cet effet n'est pas spécifique
Virus effects on plant quality and vector behavior are species specific and do not depend on host physiological phenotype by Quentin Chesnais( )

1 edition published in 2019 in English and held by 2 WorldCat member libraries worldwide

Anti-inflammatory efffect of cysteine cathepsin inhibitor stefin B is mediated by autophagy( )

1 edition published in 2018 in English and held by 2 WorldCat member libraries worldwide

Cystatins in inflammasome activation and sepsis( )

1 edition published in 2021 in English and held by 2 WorldCat member libraries worldwide

Identification of host factors potentially involved in RTM-mediated resistance during potyvirus long distance movement by Luc Sofer( )

1 edition published in 2017 in English and held by 2 WorldCat member libraries worldwide

Cystatins, metabolism and neuro inflammation( )

1 edition published in 2018 in English and held by 2 WorldCat member libraries worldwide

ǂThe ǂrole of cystatins in inflammasome activation and sepsis( )

1 edition published in 2020 in English and held by 2 WorldCat member libraries worldwide

Isolement et caractérisation du gène de la protéine capsidique du virus de la mosaïque chromée de la vigne GCMV : expression in vitro et dans des plantes transgéniques by Véronique Brault( Book )

2 editions published in 1990 in French and held by 2 WorldCat member libraries worldwide

L'ARN2 DU VIRUS DE LA MOSAIQUE CHROMEE DE LA VIGNE (GCMV) A ETE CLONE ET SEQUENCE. CET ARN, DE 4441 NUCLEOTIDES DE LONG, CODE POTENTIELLEMENT POUR UNE PROTEINE DE 146 KDA. CECI CONFIRME LES EXPERIENCES DE TRADUCTION IN VITRO DE L'ARN2 QUI ONT MONTRE QU'IL ETAIT TRADUIT SOUS FORME D'UNE POLYPROTEINE LIBERANT A PARTIR DE SON EXTREMITE C-TERMINALE LA PROTEINE CAPSIDIQUE. LE SEQUENCAGE CHIMIQUE DE L'EXTREMITE N-TERMINALE DE LA PROTEINE CAPSIDIQUE ISOLEE DE VIRIONS AINSI QUE LA RECHERCHE DE SITES DE CLIVAGE RECONNUS PAR LES PROTEASES VIRALES NOUS ONT CONDUIT A CONSIDERER DEUX SITES POTENTIELS DE CLIVAGE LIBERANT LA PROTEINE CAPSIDIQUE (R/A ET Q/A RESPECTIVEMENT EN POSITION 810/811 ET 855/856). DES CONSTRUCTIONS POUVANT CONDUIRE A LA SYNTHESE D'UNE PROTEINE AYANT L'UNE OU L'AUTRE DES DEUX EXTREMITES DETERMINEES PRECEDEMMENT ONT ETE OBTENUES. LES POLYPEPTIDES CORRESPONDANT AU SITE DE CLIVAGE R/A ONT LA MEME MIGRATION ELECTROPHORETIQUE QUE LA PROTEINE CAPSIDIQUE ISOLEE DE VIRIONS CE QUI TEND A DEMONTRER QUE C'EST LE VERITABLE SITE DE CLIVAGE DE LA CAPSIDE. CES GENES HYBRIDES ONT ETE SOUS-CLONES DANS DES VECTEURS INTERMEDIAIRES ET INTRODUITS DANS LE GENOME DE TABACS QUI EXPRIMENT LA PROTEINE CAPSIDIQUE DU GCMV
Anti inflammatory role of cystatins and mitochondrial function( )

1 edition published in 2019 in English and held by 2 WorldCat member libraries worldwide

Organisation du phloème et analyse fonctionnelle des protéines PP2 by Thibaud Cayla( )

1 edition published in 2012 in French and held by 1 WorldCat member library worldwide

The phloem is a complex tissue, made of several cell types, including companion cells and sieve elements. It plays an important role in long-distance transport and allocation of a number of metabolites and macromolecules. The sieve elements display specific structures yet uncharacterized and of unknown function. For instance the function of the phloem specific PP2 proteins (Phloem Protein 2) that have been described for long in the sieve elements is still unclear. PP2 proteins present lectin activity and bind to phloem sap proteins, suggesting a role in the transport of macromolecules in the phloem. We have investigated the function of two members of this family, PP2-A1 and PP2-A2 in the model species Arabidopsis thaliana. Different approaches have been undertaken to study these proteins: a cytological approach, the research of partners, and the study of downregulated and upregulated lines for the PP2-A1 and PP2-A2 genes. Localization studies of PP2-A1 fused to GFP-derived fluorescent tags have been realized by confocal laser scanning microscopy in the companion cells and the sieve elements; they showed that PP2-A1 presents a nucleocytoplasmic localization in the companion cells, whereas it forms fixed aggregates in the sieve elements. It suggested that PP2-A1 is anchored to the plasma membrane or to organelles inside the sieve elements. Similar results were obtained for PP2-A2. Making use of this study to accurately identify companion cells and sieve elements in vivo, and using additional subcellular fluorescent markers, we realized a fine mapping of companion cells and sieve elements in vivo. This study revealed the presence of numerous organelles of unknown identity at the periphery of the sieve elements, suggesting an important activity in those cells consistent with recent proteomics analysis of the sieve tubes. The study of plants overexpressing tagged versions of PP2-A1 and PP2-A2 enabled to observe an altered phenotype with delayed flowering and increased biomass, suggesting that PP2-A1 and PP2-A2 may play a role in long distance signalling. This work illustrates the complexity of phloem cell organization and functions, bringing new elements on long distance signalling pathway used by the plants to coordinate their growth and development
Impacts of recombinant actin expression on the structure and function of the indirect flight muscles (IFM) in transgenic Drosophila melanogaster by Véronique Brault( Book )

1 edition published in 1998 in English and held by 1 WorldCat member library worldwide

Nature du complexe viral impliqué dans le mouvement à longue distance du virus de la jaunisse du navet by Clémence Hipper( )

1 edition published in 2013 in French and held by 1 WorldCat member library worldwide

Le projet de thèse consistait à étudier le mouvement du Virus de la jaunisse du navet (TuYV) dans le système vasculaire. Le premier objectif était d'identifier la nature du complexe viral cheminant dans les tubes criblés : virions et/ou complexes ribonucléoprotéiques. L'analyse du mouvement de mutants viraux dans différentes espèces végétales, en absence ou en présence de protéines de capside de type sauvage apportées en trans, a permis de démontrer une étroite relation entre la formation de virions et le transport à longue distance. Le second objectif de cette étude portait sur l'identification de partenaires cellulaires de la protéine P4 du TuYV. Deux protéines ont été identifiées par un criblage de banques d'ADNc d'A. thaliana par le système du double hybride dans la levure, et l'analyse de leur implication dans le cycle viral a été amorcée par des expériences de localisation subcellulaire et de validation fonctionnelle in planta
Rôle des modifications posttraductionnelles des particules virales du CABYV dans la transmission par puceron by Sébastien Revollon( )

1 edition published in 2010 in French and held by 1 WorldCat member library worldwide

Previous studies on the Turnip yellows virus (TuYV) have shown that a deglycosylation of virus structural proteins inhibit the transmission of the virus by aphids. This suggested that TuYV structural proteins were glycosylated and that glycosylation could have a leading role in the vection mechanism of the virus. First, this manuscript describes how we try to dermine the presence of glycosylation on the Cucurbit aphid-borne yellows virus (CABYV) structural proteins. Different approaches were used as point mutation of potential N-glycosylated sites, immunodetection and mass-spectrometry. All this approaches lead to an absence of glycosylation on CABYV structural proteins. However a plant protein copurifying with the virus was glycosylated and could explain the results obtained in the previous studies. The second part of this manuscript describes the study of the role of different plants protein in the transmission mechanism by aphid of polervoris. A stimulating effect is observed on the transmission process, however this effect is not specific
Genome Sequence of the Pea Aphid Acyrthosiphon pisum by Stephen Richards( )

1 edition published in 2010 in English and held by 1 WorldCat member library worldwide

Aphids are important agricultural pests and also biological models for studies of insect-plant interactions, symbiosis, virus vectoring, and the developmental causes of extreme phenotypic plasticity. Here we present the 464 Mb draft genome assembly of the pea aphid Acyrthosiphon pisum. This first published whole genome sequence of a basal hemimetabolous insect provides an outgroup to the multiple published genomes of holometabolous insects. Pea aphids are host-plant specialists, they can reproduce both sexually and asexually, and they have coevolved with an obligate bacterial symbiont. Here we highlight findings from whole genome analysis that may be related to these unusual biological features. These findings include discovery of extensive gene duplication in more than 2000 gene families as well as loss of evolutionarily conserved genes. Gene family expansions relative to other published genomes include genes involved in chromatin modification, miRNA synthesis, and sugar transport. Gene losses include genes central to the IMD immune pathway, selenoprotein utilization, purine salvage, and the entire urea cycle. The pea aphid genome reveals that only a limited number of genes have been acquired from bacteria; thus the reduced gene count of Buchnera does not reflect gene transfer to the host genome. The inventory of metabolic genes in the pea aphid genome suggests that there is extensive metabolite exchange between the aphid and Buchnera, including sharing of amino acid biosynthesis between the aphid and Buchnera. The pea aphid genome provides a foundation for post-genomic studies of fundamental biological questions and applied agricultural problems
Élargissement du spectre de résistance aux potyvirus : utilisation de la plante modèle Arabidopsis thaliana by Anna Bastet( )

1 edition published in 2018 in French and held by 1 WorldCat member library worldwide

The development of genetic resistance is important to avoid viral infections in cultivated crops. In this context, translation initiation factors eIF4E have a major role in resistance to potyviruses, a family of viruses damageable to crops. Although natural resistance alleles are often used in crops breeding, there are still species devoided of such natural resistance, making it impossible to develop genetic resistance. Using the Arabidopsis thaliana-potyviruses pathosystem, I aim at developing new sources of resistances as a proof of concept before considering their application to crop species. For this, I am developing artificial resistance alleles created by directed mutagenesis before testing them for both their functionality and their resistance efficiency in plants. By combining these synthetic resistance alleles with others eIF4E factors-mediated resistance, my aim is to enlarge resistance spectrum to potyviruses as well as to increase the resistance durability. This study will make proof of the feasibility of this system to obtain large spectrum resistance plants with the perspective of extending it to cultivated plants
Partenaires et rôle dans le cycle viral des différentes formes de la protéine RT du Cucurbit aphid-borne yellows virus by Sylvaine Boissinot( )

1 edition published in 2013 in French and held by 1 WorldCat member library worldwide

Poleroviruses infect a wide range of cultivated plants such as potatoes, sugar beet and plants of Cucurbitaceae family. These viruses are restricted to phloem tissue where they replicate in nucleated cells and translocate over long distances through sieve elements. Polerovirus capsid is composed of the major coat protein (CP) and of a minor component referred to as the readthrough (RT*) protein and exposed at the outside of the particles. CP and RT proteins are essential for virus movement and transmission by aphids. The aim of this study is to identify phloem proteins interacting with viral proteins and potentially involved in viral cycle, by screening an A. thaliana companion cell (CC) cDNA library with structural proteins or protein domains of CABYV. Four genes encoding for a heat shock protein (HSP), a profilin (PRF3), a glycosyl hydrolase and the protein ”Response to low sulfur ” (LSU3) were identified and interact with the C-terminal part of the RT protein (RTC-ter) and with the RT* protein for the HSP. An additional gene encoding for the protein ALY, identified in the laboratory, by screening an aphid cDNA library with structural proteins of the Turnip yellows virus (another polerovirus) was studied. This protein has four orthologues in Arabidopsis, involved in the gene silencing mechanism against Tomato Bushy Stunt Virus. Here we show that CABYV and TuYV structural proteins interact with the four orthologues of Arabidopsis. Involvement of these candidate genes was not confirmed in Arabidopsis knock-out mutants. In functional experiments, ambiguous results were obtained with PRF3 arabidopsis mutants, and this lead me to study the interaction between PRF3 protein CABYV RT c-ter domain by FLIM, but no interaction was found so far. As all candidat interact with the RTC-ter domain, we studied more precisely the role of this domain in the viral cycle and the role of the complete RT protein. We studied the in vivo RT protein processing and its consequences on systemic movement of CABYV mutants. Using a collection of point mutations introduced in the central domain of the CABYV RT protein, we approached the site of the RT processing and proposed that this process is affected by the secondary structure around the cleavage site. We also reported for the first time the generation of a polerovirus mutant able to synthesize only the RT* protein and to incorporate it into the particle. This mutant was unable to move systemically. Conversely another mutant producing a full-length RT protein impaired in correct processing and incorporating a shorter version of the RT* protein showed very weak systemic infection. These data are strongly in favor of a role of both RT proteins in efficient CABYV movement. An inefficient virus transport was still maintained in the absence of RT proteins suggesting an RT-independent movement pathway. Based on these results, we propose a model for CABYV long-distance transport in which the complete RT protein, or its C-terminal part, acts in trans on wild-type virions to promote their efficient long-distance transport
Analyse fonctionnelle du récepteur de l'éphrine de Myzus persicae et mise en évidence de son rôle dans la transmissino du virus de la jaunisse du navet by Michaël Mulot( )

1 edition published in 2018 in French and held by 1 WorldCat member library worldwide

Poleroviruses infect a wide range of economically important plants. They are transmitted in a circulative and non-propagative mode by an insect vector, the aphid. The virus particles are acquired by aphids when ingesting the sap from an infected plant and cross successively the epithelia of the midgut and the salivary gland cells by a transcytosis mechanism that relies on the presence of unknown receptors.The ephrin receptor (Eph) is a membrane protein which contains a domain able to bind in yeast to the structural proteins of poleroviruses. By developing methods based on RNA interference, we have shown that oral acquisition of double-stranded RNA targeting Eph in the aphid Myzus persicae can reproducibly reduce polerovirus internalization into the aphid's body. Such treated aphids transmit the virus to plants with a lower efficiency. Eph could therefore function as a receptor for poleroviruses in M. persicae
Differential epitope tagging of actin in transformed Drosophila produces distinct effects on myofibril assembly and function of the indirect flight muscle( )

1 edition published in 1999 in English and held by 0 WorldCat member libraries worldwide

We have tested the impact of tags on the structure and function of indirect flight muscle (IFM)-specific Act88F actin by transforming mutant Drosophila melanogaster, which do not express endogenous actin in their IFMs, with tagged Act88F constructs. Epitope tagging is often the method of choice to monitor the fate of a protein when a specific antibody is not available. Studies addressing the functional significance of the closely related actin isoforms rely almost exclusively on tagged exogenous actin, because only few antibodies exist that can discriminate between isoforms. Thereby it is widely presumed that the tag does not significantly interfere with protein function. However, in most studies the tagged actin is expressed in a background of endogenous actin and, as a rule, represents only a minor fraction of the total actin. The Act88F gene encodes the only Drosophila actin isoform exclusively expressed in the highly ordered IFM. Null mutations in this gene do not affect viability, but phenotypic effects in transformants can be directly attributed to the transgene. Transgenic flies that express Act88F with either a 6x histidine tag or an 11-residue peptide derived from vesicular stomatitis virus G protein at the C terminus were flightless. Overall, the ultrastructure of the IFM resembled that of the Act88F null mutant, and only low amounts of C-terminally tagged actins were found. In contrast, expression of N-terminally tagged Act88F at amounts comparable with that of wild-type flies yielded fairly normal-looking myofibrils and partially reconstituted flight ability in the transformants. Our findings suggest that the N terminus of actin is less sensitive to modifications than the C terminus, because it can be tagged and still polymerize into functional thin filaments
Substitution of flight muscle-specific actin by human (beta)-cytoplasmic actin in the indirect flight muscle of Drosophila( )

1 edition published in 1999 in English and held by 0 WorldCat member libraries worldwide

The human (beta)-cytoplasmic actin differs by only 15 amino acids from Act88F actin which is the only actin expressed in the indirect flight muscle (IFM) of Drosophila melanogaster. To test the structural and functional significance of this difference, we ectopically expressed (beta)-cytoplasmic actin in the IFM of Drosophila that lack endogenous Act88F. When expression of the heterologous actin was regulated by approximately 1.5 kb of the 5' promoter region of the Act88F gene, little (beta)-cytoplasmic actin accumulated in the IFM of the flightless transformants. Including Act88F-specific 5' and 3' untranslated regions (UTRs) yielded transformants that expressed wild-type amounts of (beta)-cytoplasmic actin. Despite the assembly of (beta)-cytoplasmic actin containing thin filaments to which endogenous myosin crossbridges attached, sarcomere organization was deficient, leaving the transformants flightless. Rather than affecting primarily actin-myosin interactions, our findings suggest that the (beta)-cytoplasmic actin isoform is not competent to interact with other actin-binding proteins in the IFM that are involved in the organization of functional myofibrils
 
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Languages
French (11)

English (11)