WorldCat Identities

Grangette, Corinne

Works: 11 works in 14 publications in 2 languages and 16 library holdings
Roles: Opponent, Thesis advisor, Other
Publication Timeline
Most widely held works by Corinne Grangette
Functional Analysis of D-Alanylation of Lipoteichoic Acid in the Probiotic Strain Lactobacillus rhamnosus GG by Mónica Perea Vélez( )

2 editions published in 2009 in English and held by 3 WorldCat member libraries worldwide

Sélection in vitro et in vivo de souches probiotiques ayant des propriétés préventives dans l'allergie by Elodie Neau( )

1 edition published in 2015 in French and held by 2 WorldCat member libraries worldwide

Rôle du peptidoglycane et du récepteur NOD2 dans les capacités immunorégulatrices des lactobacilles by Elise Macho Fernandez( Book )

2 editions published in 2011 in French and held by 2 WorldCat member libraries worldwide

N genetically susceptible individuals, an inappropriate mucosal immune responseagainst the intestinal microbiota appears to be the principal mechanism leading to thepathogenesis of (including Crohn's disease and ulcerativecolitis). Therefore, modulation of the luminal contents with probiotics, mainly represented bycommensal lactic acid bacteria (lactobacilli and bifidobacteria), represents an attractivetherapeutic alternative and has already led to successful clinical trials. However, theprotective effect of probiotic bacteria clearly depends on the strains used and the precisemechanisms of action of these microorganisms are still unknown.In this study, we investigated the role of peptidoglycan and NOD2 signalling in the antiinflammatorycapacity of selected probiotic lactobacilli.In a mouse model of experimental colitis mimicking human pathology, we first showedthat the protective capacity of the anti-inflammatory strain Lactobacillus salivarius Ls33require NOD2 signalling. Since this receptor senses peptidoglycan degradation products, weprepared highly purified peptidoglycan from Ls33 and showed that systemic as well as oraladministration of this component was able to rescue mice from experimental colitis. We alsodemonstrated that the protective effect of Ls33 peptidoglycan involved the production of IL-10, the immunosuppressive indoleamine 2,3-dioxygenase pathway and the expansion ofregulatory CD103+ dendritic cells and CD4+FoxP3+ regulatory T cells within mesentericlymph nodes. Furthermore, we showed that the IL-10-producing dendritic cells induced byLs33 peptidoglycan in vitro were able to protect mice from colitis in a NOD2-dependentmanner after adoptive transfer.Since the observed anti-inflammatory properties were not obtained with peptidoglycanderived from the non-anti-inflammatory strain L. acidophilus NCFM, we conducted astructural analysis of the two peptidoglycans. While the two strains exhibited the M-tri-Lys-DAsnmuropeptide, we identified an additional muropeptide released exclusively by Ls33, theM-tri-Lys. Although both synthetic muropeptides activated NOD2 in vitro, only systemicadministration of M-tri-Lys protects mice from colitis. This protective effect was NOD2-dependent but did not require the presence of MyD88, the main adapter used by Toll-likereceptors.In conclusion, our results indicate that purified peptidoglycan and specific derivedmuropeptides are active components in probiotic lactobacilli functionality and represent anew therapeutic strategy for treating inflammatory bowel diseases
Sélection in vitro et in vivo de souches probiotiques ayant des propriétés bénéfiques contre l'inflammation, les infections et l'obésité by Jeanne Alard( Book )

2 editions published in 2017 in French and held by 2 WorldCat member libraries worldwide

Recent studies have reported that the microbiota is involved in intestinal homeostasis by contributing to the morphological development, the education of the immune system, the mechanisms of defense, and to metabolic regulation. Dysbiosis of this microbiota as well as reduction in bacterial diversity has been observed in various chronic pathologies such as chronic inflammatory diseases (IBD) and obesity. The microbiota thus constitutes a therapeutic target of choice in the management of these chronic diseases. Probiotics, which are beneficial microorganisms for the host represent therefore an interesting alternative, however their selection criteria need to be improved.In a first study, we were able to highlight the beneficial properties of a mixture of two probiotics comprising a bifidobacteria and a lactobacilli, in a murine model of high fat diet (HFD)-induced obesity (Alard et al., 2016). This probiotic mixture significantly reduced weight gain, improved inflammatory and metabolic parameters including insulin resistance, and increased intestinal expression of receptors involved in short-chain fatty acid (AGCC) recognition. It also promoted in an artificial intestinal system the production of butyrate and propionate, the two main AGCCs. The protective effects were associated with the improvement of microbiota dysbiosis, in particular the restoration of the abundance of Akkermansia muciniphila.The main objective of this thesis was then to select within a collection of 23 bacterial strains provided by PiLèJe, one or more probiotic strain (s) possessing protective properties against IBD and obesity. Immunomodulatory properties of the strains and their ability to strengthen the intestinal barrier were studied in vitro using human mononuclear blood cells and an in vitro model of epithelial permeability induced by the sensitization of a Caco-2 cells monolayer with hydrogen peroxide. Six strains were selected, five strains inducing high levels of the anti-inflammatory cytokine IL-10 and capable of restoring the intestinal barrier and a strain capable of strongly reinforcing this barrier. These strains were then evaluated in in vivo models of TNBS (2,4,6 trinitrobenzene sulfonic acid)-induced chronic and acute colitis. Interestingly, strains able to rescue mice from acute colitis did not protect as efficiently in chronic colitis and vice versa.The selection of strains or mixtures was then pursued in the context of obesity and associated metabolic diseases. We used the same criteria as previously (anti-inflammatory capacities and to restore the intestinal barrier) in addition with the capacity of the strains to limit the accumulation of lipids in an in vitro model of adipocyte differentiation based on the use of the 3T3-L1 cell line and to induce the secretion of entero-endocrine peptides, notably involved in satiety, by the use of the murine STC-1 entero-endocrine cell line. Three mixtures and one single strain were selected and evaluated in a mouse model of obesity induced by 45% HFD diet. We demonstrated the positive capacities of a mixture composed of two strains and the single strain to reduce weight gain, as well as adipose tissue inflammation.These results indicate that in vitro screenings based on the immunomodulatory properties, the capacity to restore the gut barrier, to decrease lipid accumulation and to induce gut peptides allow pre-selection of strains or mixtures exhibiting protective effects and demonstrate that the beneficial capacities of probiotics are strain-dependent and specific to the targeted models
Analyse fonctionnelle de bactéries du microbiote intestinal humain et évaluation de leurs propriétés bénéfiques contre l'obésité et l'inflammation intestinale by Bernardo Cuffaro( )

1 edition published in 2021 in French and held by 1 WorldCat member library worldwide

Des altérations dans la composition du microbiote intestinal jouent un rôle important dans le développement des pathologies chroniques, tels que l'obésité et les maladies inflammatoires de l'intestin (MICI). Cette dysbiose conduit à la perturbation de plusieurs processus biologiques clés connus pour réguler la physiologie de l'hôte, notamment l'intégrité de la barrière intestinale et les réponses immunitaires conduisant à un état pro-inflammatoire. On observe également une altération de la production d'acides gras à chaîne courte (AGCC), importants métabolites bactériens qui régulent le métabolisme de l'hôte en stimulant la sécrétion de peptides endocrines. Les interventions ciblant le microbiote représentent donc des outils intéressants dans la prise en charge de ces maladies chroniques. Les connaissances sur la composition et les fonctions du microbiote intestinal ont élargi la collection de micro-organismes pouvant avoir un rôle sur la santé de l'hôte. Ces micro-organismes sont susceptibles d'être parfaitement adaptés au tractus gastro-intestinal et dialoguer avec l'hôte. Pour ces raisons, leur évaluation en tant que probiotiques de nouvelle génération (NGP) est en pleine effervescence. Nous avons sélectionné des bactéries dominantes du microbiote intestinal et avons évalué leur robustesse aux conditions environnementales telles que la tolérance à l'oxygène et la survie au stress gastrique et observé une capacité variable selon les souches. Nous avons ensuite caractérisé leurs activités bénéfiques potentielles en utilisant différents modèles in vitro. La plupart d'entre elles ont pu induire la libération d'AGCC dans les surnageants de culture, principalement de l'acétate et dans une moindre mesure de propionate ou de butyrate. Parmi les souches criblées, une majorité a pu restaurer la barrière intestinale en utilisant un modèle in vitro de barrière épithéliale intestinale (Caco-2) et plusieurs souches ont montré un profil anti-inflammatoire après stimulation des cellules immunes humaines (PBMC) et / ou étaient de bons inducteurs de GLP-1 après stimulation de la lignée cellulaire endocrine STC-1. Fait intéressant, certaines d'entre elles ont combiné plusieurs propriétés. Nous avons ensuite évalué in vivo les souches les plus prometteuses dans différents modèles murins d'obésité et de colite. Nous avons identifié trois souches présentant des capacités prometteuses contre l'obésité. Nous avons également montré un effet souche-dépendant de l'espèce Parabacteroides distasonis contre la colite et sélectionné trois souches protectrices. Cette étude nous a apporté de plus amples informations sur la fonctionnalité des bactéries commensales ainsi que des pistes cruciales pour sélectionner des probiotiques de nouvelle génération capables de cibler les maladies chroniques inflammatoires telles que l'obésité et ou les MICI
Phagocyte activation processes in Peyer's patches and development of a strategy to analyse the gut microbiota by Cynthia Arroyo Portilla( )

1 edition published in 2021 in English and held by 1 WorldCat member library worldwide

Peyer's patches (PP) combine antigen sampling and immune response initiation sites. PP phagocytes comprise conventional dendritic cells (cDC) and lysozyme expressing monocyte-derived cells, which include dendritic cells called LysoDC and macrophages termed LysoMac. We deciphered the transcriptional landscape of all PP phagocytes from their arrival in the tissue to their final maturation state and then compared it with two types of in vivo activation, involving the Toll-like receptors (TLR) 7 and 9. We showed that the initial residence site of cDC shapes their activation profile. This was correlated with a B and regulatory T cell response upon TLR7 stimulation and a CD8+ T cell response upon TLR9 stimulation. Altogether, our study underscores the importance of targeting not only the right phagocyte subset but also the right place at the right time with the right stimulus.The microbiota is crucial for the mucosal immune system homeostasis. Variations in the composition of gut microbiota are associated with a range of health conditions. We designed a FISH-Flow multiparametric panel including nine specific probes that target the main gut bacterial phyla. We tested this panel in fecal samples from control and vancomycin-treated mice. As expected, in control mice fecal bacteria belonged mainly to Firmicutes and Bacteroidetes, whereas antibiotic treatment induced a decrease of both phyla and an increase of Verrucomicrobia. Our method allows to analyze the composition of fecal bacteria phyla in a fast, semi-quantitative and cost-effective way. This can be useful to detect microbiota dysregulation and potentially predict the development of non-communicable diseases
Rôle des serpines, inhibiteurs de protéases à serine, du microbiote digestif humain dans les maladies inflammatoires de l'intestin by Héla Mkaouar( )

1 edition published in 2019 in French and held by 1 WorldCat member library worldwide

Les inhibiteurs des protéases à sérine (Serpins) constituent une classe d'enzymes très peu étudiée chez les bactéries. Dans ce travail de thèse nous nous sommes intéressés à l'étude des serpins provenant du microbiote intestinal et l'investigation de leur potentiel anti-inflammatoire pour le traitement des maladies inflammatoires chroniques de l'intestin (MICI) chez l'homme. Pour cela nous avons identifié les serpins provenant du microbiote intestinal humain et analysé leur diversité ainsi que leur distribution entre les individus malades et sains. Ces données nous ont permis d'isoler les serpins significativement associées aux MICI. La purification de quarte d'entre elles nous a amené à démontrer qu'elles inhibent les protéases humaines impliquées dans les MICI. L'analyse biochimique et cinétique approfondie de ces protéines a montré qu'elles possèdent des propriétés originales notamment leur efficacité d'inhibition élevée. L'étude de l'effet protecteur de trois serpins chez un modèle animal de colite a démontré pour la première fois l'efficacité des serpins in vivo démontrant ainsi leur potentiel thérapeutique
Identification de nouvelles souches probiotiques à propriétés immuno-modulatrices et anti-oxydantes by Noura Larguèche( )

1 edition published in 2012 in French and held by 1 WorldCat member library worldwide

In recent years, probiotics market is boosting. Most probiotic bacteria on the market today are lactic acid bacteria. The identification of novel bacterial strains with probiotic properties is the main objective of this thesis, which is part of the Probiotic project in partnership with two industry groups and the network innovation VITAGORA
Propriétés immunomodulatrices des cellules dendritiques humaines stimulées par un produit de fermentation de bactéries probiotiques Bifidobacterium breve C50 by Audrey Granier( )

1 edition published in 2012 in French and held by 1 WorldCat member library worldwide

Notre système immunitaire a pour fonction de nous protéger des infections mais également des allergies, des cancers ou des maladies inflammatoires. Les cellules dendritiques (DC) sont au coeur de ce système en orientant les réponses immunitaires vers une voie effectrice ou tolérogène. A l'état immature, elles sont présentes, entre autres, aux interfaces avec le milieu extérieur. Elles permettent ainsi la reconnaissance des antigènes, notamment ceux présents dans le tube digestif, comme les aliments ou la flore commensale. Certaines bactéries dites probiotiques sécrètent des métabolites au cours de la fermentation définissant les produits de fermentation. Notre travail montre qu'un produit de fermentation de la souche bactérienne BbC50 (BbC50sn) activent les DC humaines grâce à sa fixation sur le récepteur TLR2 et modulent de nombreux récepteurs à leurs surfaces. Les DC ainsi maturées peuvent activer des lymphocytes T CD4+ et CD8+ et également induire des lymphocytes T régulateurs CD4+ et CD8+
Étude de l'implication d'une voie MyD88/IL-22 dans le contrôle de la colonisation par la bactérie segmentée filamenteuse by Marion Picard( )

1 edition published in 2017 in French and held by 1 WorldCat member library worldwide

The mammalian intestine is heavily colonized by a huge microbial community. During a long coevolution process, the host has evolved mutualistic relationships with its microbiota. Thes relationships, mainly based on metabolstic advantages, also allow the full maturation of the host immune system, which development is initiated in utero by a genetic program. In mice, only SFB has been yet described to display strong immunostimulant properties allowing the coordination of a large panel of immune responses, more specifically IgA and Th17 responses, which preferential induction sites might be the Peyer's patches. The first part of my thesis contributed to complete a work dedicated to the characterization of the IgA et Th17 responses induced by SFB. We have underscored SFB capacity to stimulate the post-natal maturation of isolated lymphoid follicles and also tertiary lymphoid tissues that would substitute to Peyer's patches as inductors sites of both IgA and Th17 responses induced by SFB. However, this microbiote also constitutes a potential antigenic threat for the host integrity. Notably, the SFB could provoke chronic deleterious inflammation in peripheric compartment in genetically predisposed individuals. It suggests the establishment of highly regulated mechanisms regulating SFB colonization, to avoid compromising events for the host homeostasis. In a second part of my thesis, with the help of immunodeficient axenic mice, we have shown a role of TLR signaling pathways in the control of SFB colonization. However, antimicrobial peptides, IgA, IL-17 or IL-22 seem to be involved int eh control of SFB colonization
Probiotique et autophagie : exploration de l'impact possible sur la maladie de Crohn by Mazen Zaylaa( )

1 edition published in 2018 in French and held by 1 WorldCat member library worldwide

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French (11)

English (3)