WorldCat Identities

Hubert-Luco, Sophie

Overview
Works: 1 works in 2 publications in 1 language and 3 library holdings
Roles: Author
Publication Timeline
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Most widely held works by Sophie Hubert-Luco
Impact de la protéine de matrice des lyssavirus sur la réponse de l'hôte by Sophie Hubert-Luco( Book )

1 edition published in 2012 in French and held by 2 WorldCat member libraries worldwide

The matrix protein (M) of lyssaviruses plays some pivotal roles during the viral life circle. This study aimed at describing the impact of the M protein on host response. We described and characterized a novel cell protein targeted by the M protein of field isolates of lyssaviruses : RelAp43. We demonstrated that it exhibits all the properties of a class I member of the NF-kB family of transcription factors. We have shown that RelAp43 can modulate gene expression, in particular for genes involved in innate immunity like IFN-p. RelAp43 is able to modigy the equilibrium between the differents NF-kB dimers, by limiting p50 degradation and inhibiting the association between p66/RelA and p50. This work demonstrated that RelAp43 is specifically targeted by M protein from field isolates of lyssaviruses, contrary to vaccine strains. This interaction induces an inhibition of the NF-kB pathway and of IFN-ß expression. We established that residues 77 and 104 of the M protein were necessary to its interaction with RelAp43, while positions 100 and 110 are crucial of the host response inhibition. We also studied the role of protein M multimerisation on its modulation of host response. We developed a protocol of production and purification of M protein in bacteria. Using biophysical approaches (analytical ultracentrifugation and dynamic light scattering), we compared the biophysical properties of M protein in term of multimerisation. We tried to correlate their oligomerisation abilities with some biological observations about the induction of apoptosis and the modulation of NF-kB signaling
 
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Languages
French (1)