60f Montes Usategui, Mario [WorldCat Identities]
WorldCat Identities

Montes Usategui, Mario

Overview
Works: 13 works in 25 publications in 3 languages and 25 library holdings
Roles: Author, Other
Publication Timeline
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Most widely held works by Mario Montes Usategui
Multichannel optical correlators : analysis, evaluation, and development of procedures for pattern recognition by Mario Montes Usategui( Book )

5 editions published between 1995 and 2019 in English and held by 4 WorldCat member libraries worldwide

"Los objetivos del trabajo son, en primer lugar, analizar las limitaciones que presenta la correlación monocanal para tratar con problemas complejos de reconocimiento de imágenes. Con este propósito se ha adaptado el formalismo de las regiones de decisión al caso particular de la correlación optica. El análisis muestra asimismo la forma de resolver los problemas encontrados, mediante la utilización de sistemas que realicen múltiples correlaciones (correladores multicanal). Se han desarrollado procedimientos para sacar provecho de la potencia de estos sistemas y se ha mostrado la superioridad frente a los sistemas de correlación unica.Se han estudiado los problemas encontrados en la construcción práctica de estos dispositivos y se han propuesto soluciones a algunos de ellos. Finalmente, se explora la posibilidad de realizar las multiples correlaciones necesarias mediante una arquitectura de correlación iterativa." -- TDX
Análisis de la influencia de las aberraciones del sistema difractor en el reconocimiento de imágenes por correlación óptica by Julio D Pérez Tudela( )

3 editions published in 2006 in Spanish and held by 3 WorldCat member libraries worldwide

Momentum measurements of single-beam traps and quantitative holographic experiments two sides of the same coin by Arnau Farré Flaquer( )

3 editions published in 2012 in English and held by 3 WorldCat member libraries worldwide

Manipulació òptica i seguiment amb presició nanomètrica per estudis de transport en cèl·lules vives by Carol López-Quesada( )

2 editions published in 2012 in Catalan and held by 2 WorldCat member libraries worldwide

Investigation on force detection methods for optical trapping and sensing inside living cells by Josep Mas Soler( Book )

2 editions published between 2016 and 2017 in Spanish and English and held by 2 WorldCat member libraries worldwide

Optical Tweezers (OT) are a light-based non-invasive tool that has played an important role during the last decades in the field of biophotonics, together with the advances in optical microscopy techniques. Optical trapping and micromanipulation of microscopic objects is nowadays possible with tightly focused laser beams, with the capability of measuring forces acting on the trapped particle in the order of piconewtons. OT have found numerous applications in the field of biophysics, and more specifically in molecular motor studies. One of the open challenges in the field is measuring molecular motor forces in their natural (in vivo) environment: the interior of living cells. Due to the complex mechanical properties of the cell cytoplasm, standard trap force calibration methods do not apply in this environment. In this thesis we investigate two recent optical force calibration methods: active-passive calibration in viscoelastic media, and direct force measurements via light momentum changes. The two methods are tested and cross-validated inside living cells, and an application involving intracellular molecular motor stall force measurements is demonstrated. The obtained results prove the possibility of measuring forces inside the cell cytoplasm with holographic tweezers, even with an object-independent trap force calibration when the conditions for the momentum-based technique are fulfilled. We also present an algorithm for manipulating groups of optical traps with minimal computational cost, using random mask multiplexing combined with iterative computer-generated holograms for dynamic holographic optical tweezers
Influence of experimental parameters on the laser heating of an optical trap by Frederic Català( )

1 edition published in 2017 in English and held by 2 WorldCat member libraries worldwide

Holographic optical tweezers systems for biophysical experiments by Jordi Andilla i Salla( )

2 editions published in 2008 in English and held by 2 WorldCat member libraries worldwide

Implementation of the direct force measurement method in optical tweezers by Frederic Català i Castro( Book )

2 editions published in 2019 in English and held by 2 WorldCat member libraries worldwide

Mechanics is the branch of physics that studies movement and force, and plays an evident role in life. The swimming dynamics of bacteria in search of nutrients, organelle transport by molecular motors or sensing different kinds of stimuli by neurons, are some of the processes that need to be explained in terms of mechanics. At a human scale, distance and force can be measured with a ruler and a calibrated spring. However, assessing these magnitudes may become an important challenge at a micron scale. Among several techniques, optical tweezers stand out as a non-invasive tool that is capable of using light to grab micron-sized particles and measuring position and force with nanometer (10(-9) and femto-Newton (10(-15) accuracy. Small specimens, such as a bacterium or a cell membrane, can be trapped and effectively manipulated with a focused laser beam. Light momentum exchanged with the trapped sample can be used for eventually measuring the otherwise inaccessible forces that govern biological processes. Optical tweezers have enabled, after trapping cell vesicles in vivo, to measure the pulling force exerted by molecular motors, such as kinesin. Flagellar propulsion forces and energy generation have been investigated by optically trapping the head of a bacterium. Cell membranes have been deformed with optical tweezers and the underlying tension determined. However, the exact forces exerted by optical tweezers are difficult to measure beyond the in vitro approach. In order to calibrate the optical traps, the trapped samples often need to be spherical or present some degree of symmetry, it is important to bear information on the experimental parameters, and one needs high control of several variables that determine the trapping dynamics, such as medium homogeneity and temperature. A cutting-edge method, developed in the Optical Trapping Lab – BiOPT, from the Universitat de Barcelona, targets the light-momentum change as a direct reading of the force exerted by an optical trap. This frees experiments from the necessity of calibrating the optical traps, and makes possible to perform accurate force measurement experiments in vivo and involving irregular samples. In my PhD thesis, the direct force detection method for optical tweezers has been implemented and tested in some of such situations. I first give a technical description of the set-up used for the experiments. The use of a spatial light modulator (SLM) for holographic optical tweezers (HOTs), a piezo-electric platform to induce drag forces, and the trapping laser emission characteristics, are explained in detail. The light-momentum set-up is tested against certain situations deviating from the ideal performance and some steps for optimization of several effects are analyzed. Backscattering light loss is quantified through experiments and numerical simulations and finally assessed to account for an average ±5% uncertainty in force measurements. Then, the method is used to measure forces on irregular samples. First, arbitrary systems composed of microspheres of different kinds are collectively treated as irregular samples, in which the global momentum exchanged with the trapping beam coincides with the total Stokes-drag force. Second, pairs of optical tweezers are used to stably trap cylinders of sizes from 2 milimicras to 50 milimicras and measure forces in accordance with slender-body hydrodynamic theory. Another aspect of the thesis deals with the temperature change induced by water absorption of IR light, which is one of the major concerns within the optical trapping community. As main reasons, accurate knowledge of local temperature is needed for understanding thermally-driven processes, as well as eventual damage to live specimens. Here we use direct force measurements to detect changes in viscosity that are due to laser heating, and compare the results with heat transport simulations to discuss the main conclusions on this effect. The last goal of my thesis has been the implementation of the method inside tissue. The laser beam is affected by the scattering structures present in vivo, such as refractive index mismatches throughout different cells, nuclei, cell membranes or vesicles. As a primary result, despite the trapping beam is captured beyond 95%, I quantified this effect to result in an increase in the standard deviation of force measurements around ±20%. The approach has consisted in comparing the trapping force profiles of spherical probes in vitro (water) and in vivo (zebrafish embryos). To conclude, I here demonstrate that the direct force measurement method can be applied in an increasing number of experiments for which trap calibration becomes intricate or even impossible. Quantitative measurements become feasible in samples with unknown properties, the more important examples being arbitrary, non-spherical samples and the interior of an embryonic tissue
Digitalización tridimensional de objetos para la generación automática de imágenes de referencia en procesos de correlación óptica by Francisco Javier Rodríguez Miguel( )

1 edition published in 2002 in Spanish and held by 1 WorldCat member library worldwide

Video-based calibration of holographic optical traps by Dorian Treptow( )

1 edition published in 2011 in English and held by 1 WorldCat member library worldwide

Disseny d'un algoritme per al càlcul de distàncies a partir de tècniques d'estèreo visió by Ignasi Sos Bravo( )

1 edition published in 2003 in Catalan and held by 1 WorldCat member library worldwide

Back Focal Plane Interferometry for Holographic Optical Traps by Jade Martínez Llinàs( )

1 edition published in 2011 in English and held by 1 WorldCat member library worldwide

English: Since its invention in 1986, optical trapping as a tool to manipulate microparticles non-invasively has found many applications in physics and biology. Force measurement on biomaterials is a potential application of optical tweezers which requires precise position detection. Back focal plane interferometry has become a commonly precise method for position detection, but it is limited to single trap calibration. However, the study of complex biological systems requires simultaneous multiple trap manipulation. Time-sharing a trap at kHz by acousto-optic-deflectors produces multiple independent tweezers, but limited to two dimensions. In this work, we aim to combine the versatility of digital holography for simultaneous multiple manipulation with the precision of back focal plane interferometry for force measurement. A holographic optical system set-up has been built and used to generate two traps with orthogonal polarizations: a fixed trap useful for measuring forces, and a holographic tweezer which allows dynamic manipulation. This method represents a step towards simultaneous manipulation with many holographic traps and precise position detection with only one trap
Escáner de sólidos por captura de imágenes by Alberto Ayala Bernal( )

1 edition published in 1999 in Spanish and held by 1 WorldCat member library worldwide

 
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Alternative Names
Mario Montes-Usategui researcher

Mario Montes-Usategui wetenschapper

Montes, Mario (Montes Usategui)

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