WorldCat Identities

Ghigho, Jean-Marc

Works: 1 works in 1 publications in 1 language and 1 library holdings
Roles: Other
Publication Timeline
Most widely held works by Jean-Marc Ghigho
ARN régulateurs de Staphylococcus aureus : Rôle de RsaA dans la formation du biofilm et de la capsule, niveaux d'expression des ARN dans les prélèvements cliniques by Claire Lays( )

1 edition published in 2012 in French and held by 1 WorldCat member library worldwide

Staphylococcus aureus is responsible for many nosocomial and communityinfections with a diversity linked to the expression of many virulence factors. Their expression istemporally coordinated during infection by transcription factors, two- component systems but alsoregulatory RNAs called non coding RNAs (ncRNAs). A large number of potential ncRNAs waspredicted in the genome of S. aureus but their function remains unknown.The first aim of this thesis was to characterize the role of one ncRNA identified by our team,RsaA, in the regulation of bacterial virulence. Several in vitro approaches allowed todemonstrate that RsaA activates biofilm formation and inhibits the bacterial capsule synthesis,with an impact on the bacterial opsonophagocytosis and internalization into macrophages. RsaArepresses the translation of mgrA mRNA by an antisense mechanism. mgrA codes for atrancriptional regulator known to repress biofilm formation but to activate capsule synthesis. Inparallel, RsaA represses the translation of the yabJ-spoVG operon, an activator of capsuleproduction. In this way, RsaA enhances biofilm formation and represses capsule synthesis.The second objective was to characterize the in vivo expression of RNA III and RsaA, E, G, HsRNAs in samples of acute infections (cutaneous absesses), chronicle infections (cystic fibrosis)and nasal carriage. The expression study (quantitative RT-PCR) allowed to demonstrate that allsRNA are expressed into samples but they have a high expression variability depending ofinfectious samples compared to asymptomatic carriage
Audience Level
Audience Level
  Kids General Special  
Audience level: 0.96 (from 0.96 for ARN régul ... to 0.96 for ARN régul ...)

French (1)