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Adrenergic receptor protocols

Author: Curtis A Machida
Publisher: Totowa N.J : Humana Press, cop. 2000.
Series: Methods in molecular biology (Clifton, N.J.), 136.
Edition/Format:   Print book : EnglishView all editions and formats
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Adrenergic Receptor Protocols consists of 35 chapters dealing with va- ous aspects of adrenergic receptor analyses, including the use of genetic, RNA, protein expression, transactivator, second  Read more...

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Document Type: Book
All Authors / Contributors: Curtis A Machida
ISBN: 0896036022 9780896036024
OCLC Number: 491032940
Description: 1 vol. (XIV-577 p.) : ill. ; 24 cm.
Contents: Part I. Genetic Analysis. Construction of Libraries for Isolation of Adrenergic Receptor Genes, Margaret A. Scofield, Jean D. Deupree, and David B. Bylund. Isolation of Adrenergic Receptor Genes, Margaret A. Scofield, Jean D. Deupree, and David B. Bylund. Analyses of Adrenergic Receptor Sequences, Jean D. Deupree, Margaret A. Scofield, and David B. Bylund. Polymerase Chain Reaction Screening of Genomic Libraries for Adrenergic Receptor Genes, Dianne M. Perez and Michael J. Zuscik. Solution-Phase Library Screening for Identification of Rare Adrenergic Receptor Clones, Dianne M. Perez and Michael J. Zuscik. Genetic Polymorphisms of Adrenergic Receptors, Ian P. Hall, Amanda P. Wheatley, and Jane C. Dewar. Part II. RNA Analysis. Northern Blot Analyses Detecting Adrenergic Receptor mRNAs, Judith C. W. Mak. Ribonuclease Protection Assay for the Detection of b1-Adrenergic Receptor RNA, Yong-Feng Yang and Curtis A. Machida. Determination of Adrenergic Receptor mRNAs by Quantitative Reverse Transcriptase-Polymerase Chain Reactions, Stefan Engelhardt and Martin J. Lohse. Nuclear Run-On Assays for Measurement of Adrenergic Receptor Transcription Rate, Zhuo-Wei Hu and Brian B. Hoffman. Primer Extension Methods for Determination of b1-Adrenergic Receptor mRNA Start Sites, Yi-Tang Tseng and James F. Padbury. Part III. Expression Analysis. Use of Eukaryotic Vectors for the Expression of Adrenergic Receptors, Stephane Schaak, Jean-Christophe Devedjian, and Herve Paris. Expression of b-Adrenergic Receptors in Recombinant Baculovirus-Infected Insect Cells, A. Donny Strosberg and Jean-Luc Guillaume. Expression of b-Adrenergic Receptors in E. coli, Ralf Jockers and A. Donny Strosberg. Use and Pharmacological Analysis of Established and Transfected Cell Lines Expressing Adrenergic Receptors, Hongying Zhong and Kenneth P. Minneman. Transient Transfection and Adrenergic Receptor Promoter Analysis, Yi-Tang Tseng and James F. Padbury. Antisense RNA/DNA-Based Techniques to Probe AdrenergicReceptor Function, Hsien-yu Wang, Fubao Lin, and Craig C. Malbon. Targeted Disruption of Adrenergic Receptor Genes, Daniel K. Rohrer. Part IV. Receptor and Second Messenger Analysis. Development of Antibodies to Adrenergic Receptors, Suleiman W. Bahouth. Western Blot Detection of Adrenergic Receptors, Suleiman W. Bahouth. UV Crosslinking of Adrenergic Receptors and Ligands: Detection by SDS-PAGE, Michael K. Sievert, John F. Resek, Zhongren Wu, Yajing Rong, and Arnold E. Ruoho. Detection of b-Adrenergic Receptors by Radioligand Binding, Cheryl D. Dunigan, Patricia K. Curran, and Peter H. Fishman. Assessing Adrenergic Receptor Conformation Using Chemically Reactive Fluorescent Probes, Anne Dam Jensen and Ulrik Gether. Biochemical Methods for Detection and Measurement of Cyclic AMP and Adenylyl Cyclase Activity, Steven R. Post, Rennolds S. Ostrom, and Paul A. Insel. Assay of Arachidonic Acid Release Coupled to a1- and a2-Adrenergic Receptors, Lincoln Edwards and Paul Ernsberger. Patch-Clamp Recording Methods for Examining Adrenergic Regulation of Potassium Currents in Ocular Epithelial Cells, Jennifer S. Ryan, Chanjuan Shi, and Melanie E. M. Kelly. Part V. Transactivator Analysis. Southwestern Blots for Detection of a DNA Binding Protein Recognizing the a1B-Adrenergic Receptor Gene Promoter, Bin Gao and George Kunos. DNase I Footprinting Analysis of Transcription Factors Recognizing Adrenergic Receptor Gene Promoter Sequences, Bin Gao and George Kunos. Electrophoretic Mobility Shift Assay for Detection of DNA Binding Proteins Recognizing b-Adrenergic Receptor Gene Sequences, Philbert Kirigiti and Curtis A. Machida. Determination of mRNA Stability and Characterization of Proteins Interacting with Adrenergic Receptor mRNAs, Burns C. Blaxall and J. David Port. Part VI. Microscopy Analysis. Use of Immunohistochemistry and Confocal Microscopy in the Detection of Adrenergic Receptors, Ruth L. Stornetta. Distribution of a2A- and a2C-Adrenergic Receptor Immunoreactivity in th
Series Title: Methods in molecular biology (Clifton, N.J.), 136.
Responsibility: edited by Curtis A. Machida.

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