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Genre/Form: | Thèses et écrits académiques |
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Material Type: | Document, Thesis/dissertation |
Document Type: | Computer File |
All Authors / Contributors: |
Erwan Denis; Martin Kreis; Yves Deveaux; Catherine Damerval; Pierre Carol; Teva Vernoux; Jean-Christophe Palauqui; Université Paris-Sud (1970-2019).; Ecole doctorale Sciences du Végétal (1992-2015 / Orsay, Essonne). |
OCLC Number: | 819173707 |
Notes: | Thèse soutenue sur un ensemble de travaux. Titre provenant de l'écran-titre. |
Description: | 1 online resource |
Contents: | WOX14 controls vascular bundle initiation and differentiation in Arabidopsis / Denis, Erwan, Kreis, Martin, Pinto Gonçalves, Beatriz, Rosa, Sarah, Claisse, Gaelle, Deveaux, Yves. -- , . -- The WOX14 control of vascular bundle formation and floral transition is mediated by active Gibberellins / Denis, Erwan, Mary, Viviane, Kreis, Martin, Deveaux, Yves. -- , . |
Responsibility: | Erwan Denis ; sous la direction de Martin Kreis et de Yves Deveaux. |
Abstract:
Whilst primary meristems are initiated during embryogenesis, in higher plants additionalsecondary meristems initiate post-embryonically and contribute to the plant architecture andthe vascular strand development. Differentiation of the plant vascular cambium into xylemand phloem was shown to be regulated by cell to cell communication. The large CLE(CLAVATA3/ENDOSPERM SURROUNDING REGION related) signaling peptide familyand the WOX (WUSCHEL-LIKE HOMEOBOX) transcription factor family are thought to beconserved regulators of stem cell fate. In this thesis we report the presence of supernumeraryvascular bundles in the young inflorescence stem of the wox14 mutant. Our data indicate thatWOX14 prevents additional cambium cell to differentiate into vascular bundles during floraltransition. Moreover, the data suggest that vascular differentiation and floral transition arelinked. Consistently, WOX14 expression is induced within the connecting vascular strandduring floral transition. Furthermore, the application of gibberellins (GA) fully rescued boththe floral transition and the vascular bundle phenotype of the wox14 mutants. A detail analysisof GA biosynthesis and target genes showed that WOX14 controls the amount of bioactiveGA within the vasculature. However, WOX14 is also specifically expressed in the phloem ofthe inflorescence stem indicating a function in late vascular bundle development. We alsoshowed that not only WOX4 but also WOX14 are the target of the CLE41 peptide duringvascular development. Furthermore, the data indicate that another CLE gene, namely CLE46,is misregulated in the wox14 mutant. These results suggest that CLE46 might be the firstidentified CLE signal from the xylem that impacts vascular differentiation.
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