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Ancient DNA : methods and protocols

Author: Beth Alison ShapiroAxel BarlowPeter D HeintzmanMichael HofreiterJohanna L A PaijmansAll authors
Publisher: New York, NY : Humana Press, 2019.
Series: Methods in molecular biology (Clifton, N.J.), v. 1963.
Edition/Format:   eBook : Document : English : Second editionView all editions and formats

This fully updated second edition explores protocols that address the most challenging aspects of experimental work in ancient DNA, such as preparing ancient samples for DNA extraction, the DNA  Read more...


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Genre/Form: Electronic books
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Beth Alison Shapiro; Axel Barlow; Peter D Heintzman; Michael Hofreiter; Johanna L A Paijmans; André E R Soares
ISBN: 9781493991761 1493991760
OCLC Number: 1090538195
Notes: Includes bibliographical references and index.
Description: 1 online resource (x, 216 pages) : illustrations (some color).
Contents: 1. Setting Up an Ancient DNA Laboratory Tara L. Fulton and Beth Shapiro 2. Pretreatment: Removing DNA Contamination from Ancient Bones and Teeth Using Sodium Hypochlorite and Phosphate Petra Korlevic and Matthias Meyer 3. Pretreatment: Improving Endogenous Ancient DNA Yields Using a Simple Enzymatic Pre-Digestion Step Hannes Schroeder, Peter de Barros Damgaard, and Morten E. Allentoft 4. Extraction of Highly Degraded DNA from Ancient Bones and Teeth Jesse Dabney and Matthias Meyer 5. Sampling and Extraction of Ancient DNA from Sediments Laura S. Epp, Heike H. Zimmermann, and Kathleen R. Stoof-Leichsenring 6. Extraction of Ancient DNA from Plant Remains Nathan Wales and Logan Kistler 7. DNA Extraction from Keratin and Chitin Paula F. Campos and M. Thomas P. Gilbert 8. Double-Stranded Library Preparation for Ancient and Other Degraded Samples Kirstin Henneberger, Axel Barlow, and Johanna L.A. Paijmans 9. A Method for Single-Stranded Ancient DNA Library Preparation Marie-Theres Gansauge and Matthias Meyer 10. Sequencing Library Preparation from Degraded Samples for Non-Illumina Sequencing Platforms Renata Martins, Marie-Louise Kampmann, and Daniel W. Foerster 11. Whole-Genome Capture of Ancient DNA Using Homemade Baits Gloria Gonzalez-Fortes and Johanna L.A. Paijmans 12. Generating RNA Baits for Capture-Based Enrichment Noah Snyder-Mackler, Tawni Voyles, and Jenny Tung 13. Hybridization Capture of Ancient DNA Using RNA Baits Andre E.R. Soares 14. Application of Solid-State Capture for the Retrieval of Small-to-Medium Sized Target Loci from Ancient DNA Johanna L.A. Paijmans, Gloria Gonzalez-Fortes, and Daniel W. Foerster 15. Targeted PCR Amplification and Multiplex Sequencing of Ancient DNA for SNP Analysis Saskia Wutke and Arne Ludwig 16. Targeted Amplification and Sequencing of Ancient Environmental and Sedimentary DNA Ruth V. Nichols, Emily Curd, Peter D. Heintzman, and Beth Shapiro 17. Authentication and Assessment of Contamination in Ancient DNA Gabriel Renaud, Mikkel Schubert, Susanna Sawyer, and Ludovic Orlando 18. Assembly of Ancient Mitochondrial Genomes without a Closely Related Reference Sequence Christoph Hahn
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1963.
Responsibility: edited by Beth Shapiro, Axel Barlow, Peter D. Heintzman, Michael Hofreiter, Johanna L. A. Paijmans, André E. R. Soares.


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