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Antibody engineering protocols

Author: Sudhir Paul
Publisher: Totowa, N.J. : Humana Press, ©1995.
Series: Methods in molecular biology (Clifton, N.J.), v. 51.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

This collection of developed methods for producing new antibody reagents by immunization and recombinant DNA techniques contains ready-to-use protocols that illuminate current areas of research on  Read more...

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Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Sudhir Paul
ISBN: 0896032752 9780896032750 9781592595389 1592595383
OCLC Number: 32626831
Description: xii, 450 pages : illustrations ; 23 cm.
Contents: SEQHUNT : a program to screen aligned nucleotide and amino acid sequences / George Johnson, Tai Te Wu, Elvin A. Kabat --
Molecular modeling of antibody-combining sites / David M. Webster, Anthony R. Rees --
Structure and properties of human immunoglobulin light-chain dimers / Fred J. Stevens, Marianne Schiffer --
Crystallographic and chromatographic methods for study of antibody light chains and other proteins / Marianne Schiffer, Fred J. Stevens --
Detection of human variable gene family expression at the single-cell level / Moncef Zouali --
Purification of reduced and alkylated antibody subunits / Mei Sun, Sudhir Paul --
Murine monoclonal antibody development / Donald R. Johnson --
Comparative properties of polyclonal and monoclonal antibodies / L. Scott Rodkey --
Evaluation of antibody clonality / L. Scott Rodkey --
Affinity immunoblotting / Keith A. Knisley, L. Scott Rodkey --
Epitope and idiotope mapping using monoclonal antibodies / Srinivas Kaveri --
Anti-idiotypic antibodies that mimic opioids / Jay A. Glasel, Dianne Agarwal --
Catalytic antibodies : structure and possible applications / Howard Amital, Ilan Tur-Kaspa, Zeev Tashma, Israel Hendler, Yehuda Shoenfeld --
Preparation and assay of acetylcholinesterase antibody / Alain Friboulet, Ladan Izadyar-Demichèle --
DNA hydrolysis by antibodies / Alexander G. Gabibov, Oxana Makarevitch --
Screening strategies for catalytic antitransition-state analog antibodies / Alfonso Tramontano --
Expression of chimeric immunoglobulin genes in mammalian cells / Sergey M. Deyev, Oleg L. Polanovsky --
Single-chain anti-DNA Fv / Michael Polymenis, B. David Stollar --
Molecular cloning of antiground-state proteolytic antibody fragments / Qing-Sheng Gao, Sudhir Paul --
Cloning and bacterial expression of an esterolytic sFv / Rodger G. Smith, Mark T. Martin, Rosa Sanchez, John H. Kenten --
Site-directed mutagenesis of antibody-variable regions / Qing-Sheng Gao, Sudhir Paul --
Synthetic antibody gene libraries for in vitro affinity maturation / Su-jun Deng, C. Roger MacKenzie, Saran A. Narang --
Chaperonins in phage display of antibody fragments / Eskil Söderlind, Marta Dueñas, Carl A. Borrebaeck --
Phage-display libraries of murine and human antibody fab fragments / Jan Engberg, Peter Sejer Andersen, Leif Kofoed Nielsen, Morten Dziegiel, Lene K. Johansen, Bjarne Albrechtsen --
Selection of human immunoglobulin light chains from a phage-display library / Sonia Tyutyulkova, Qing-Sheng Gao, Sudhir Paul --
Purification of antibody light chains by metal affinity and protein L chromatography / Sonia Tyutyulkova, Sudhir Paul --
Rapid purification of recombinant antibody fragments for catalysis screening / Han Huang, Brian Fichter, Robert Dannenbring, Sudhir Paul --
Assay of radiolabeled VIP binding and hydrolysis by antibodies / Han Huang, Sudhir Paul --
Methods of measuring thyroglobulin and peptide-methylcoumarinamide hydrolysis by autoantibodies / Lan Li, Ravishankar Kalaga, Srinivas Kaveri, Sudhir Paul --
Radiolabeling of antibodies for therapy and diagnosis / Janina Baranowska-Kortylewicz, Glenn V. Dalrymple, Syed M. Quadri, Katherine A. Harrison.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 51.
Responsibility: edited by Sudhir Paul.
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