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Aptamers for analytical applications : affinity acquisition and method design

Author: Yiyang Dong
Publisher: Weinheim, Germany Wiley-VCH [2018]
Edition/Format:   Print book : EnglishView all editions and formats
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Genre/Form: Aufsatzsammlung
Document Type: Book
All Authors / Contributors: Yiyang Dong
ISBN: 9783527342679 3527342672
OCLC Number: 1085552662
Description: xix, 406 Seiten : Illustrationen
Contents: About the Author xvForeword xviiPreface xix1 Introduction of SELEX and Important SELEX Variants 1Yiyang Dong, ZhuoWang, SaiWang, YehuiWu, YufanMa, and Jiahui Liu1.1 SELEX 11.2 Negative SELEX and Its Analogs 31.3 One-Round SELEX 51.4 CE-SELEX 61.5 Microfluidic SELEX 81.6 Cell-SELEX 101.7 In Silico-SELEX 121.8 Post-SELEX and In Chemico-SELEX 141.9 Auto-SELEX 171.10 Primer-Free SELEX 171.11 Genomic SELEX 181.12 Photo-SELEX 191.13 qPCR-SELEX 191.14 Perspectives 20References 212 In Chemico Modification of Nucleotides for Better Recognition 27Przemyslaw Jurek, MartaMatusiewicz,MaciejMazurek, and Filip Jelen2.1 Introduction 272.1.1 Beyond ATGC 272.1.2 The Scope of This Chapter 292.2 Modified Functional Nucleic Acids 302.2.1 The "Hows" 302.2.1.1 Post-SELEX Optimization 302.2.1.2 In-line Modifications 302.2.2 The "Whys" 312.2.2.1 The Hurdles 312.2.2.2 The Gains 322.2.3 The "Ifs" 332.3 Backbone Modifications 352.3.1 2'-OH Modifications 362.3.2 Phosphodiester Bond Modifications 362.3.3 Xeno Nucleic Acids 382.3.3.1 TNA 392.3.3.2 FANAs 392.3.3.3 HNA, CeNA, LNA, ANA 392.3.3.4 Other Modifications 402.4 Nucleobase Modifications 402.4.1 General Information 402.4.2 Modified Aptamers and Catalysts 422.4.2.1 Introduction of Cationic Moieties 422.4.2.2 Catalysts with Protein-like Sidechains 432.4.2.3 Nucleobase-linked Nucleobases 442.4.2.4 Glycans Targeting with Boronic Acids 442.4.2.5 "Click Chemistry"-Based Versatile Approach 452.4.2.6 Nonenzymatic Selection - X-aptamers 452.4.2.7 Slow Off-rate Modified Aptamers 462.5 Aptamers with Expanded Genetic Alphabet 482.5.1 GACTZP Aptamers 482.5.2 Aptamers with a Hydrophobic Fifth Base 502.6 Summary 522.A Appendix 52References 683 Immobilization of Aptamers on Substrates 85Annalisa De Girolamo, Maureen McKeague,Michelangelo Pascale, Marina Cortese, andMaria C. DeRosa3.1 Introduction 853.2 Methods for Immobilization of Aptamers 873.2.1 Physical Adsorption 873.2.2 Covalent Binding 883.2.2.1 Covalent Immobilization of Activated Aptamers on a Functionalized Surface 883.2.2.2 Covalent Immobilization of Modified Aptamers on Activated Surfaces 923.2.2.3 Covalent Immobilization by Entrapment 953.2.2.4 Covalent Immobilization by Electrografting 973.2.3 Self-assembled Monolayers 983.2.4 Avidin-Biotin Binding (Affinity Coupling) 1003.2.5 Electrochemical Adsorption 1013.2.6 Hybridization 1013.3 Immobilization of Aptamers on Substrates for Diagnostic Applications 1023.3.1 Flat Gold 1023.3.1.1 Surface Plasmon Resonance Detection 1093.3.1.2 Electrochemical Detection 1093.3.2 Solid Phase 1113.3.2.1 Optical Detection 1123.3.2.2 Sample Cleanup 1143.3.3 Nanomaterials 1153.4 Future Perspectives on New Substrates and New Immobilization Chemistries 1163.5 Conclusions 117References 1194 Characterization of Aptamer-Ligand Complexes 127RebecaMiranda-Castro, Noemi de-los-Santos-Alvarez, and Maria J. Lobo-Castanon4.1 Introduction 1274.2 Equilibrium Characterization:Thermodynamics 1284.2.1 Basic Principles 1284.2.2 Separation-Based Methods 1334.2.2.1 Equilibrium Dialysis and Related Techniques 1334.2.2.2 High-Performance Liquid Chromatography 1354.2.2.3 Electrophoresis 1364.2.3 Direct Methods 1374.2.3.1 Isothermal Titration Calorimetry 1384.2.3.2 Fluorescence-Based Methods 1404.3 Kinetic Characterization 1464.3.1 Heterogeneous Methods 1484.3.1.1 Surface Plasmon Resonance 1484.3.1.2 Electrochemical Impedance Spectroscopy 1524.3.2 Homogeneous Methods 1544.3.2.1 Rotating Droplet Electrochemistry 1544.3.2.2 Capillary Electrophoresis 1574.3.2.3 Nanopore-Based Studies 1594.4 Concluding Remarks 162Acknowledgments 163References 1645 Utilization of Aptamers for Sample Preparation in Analytical Methods 173Zhiyong Yan and Yang Liu5.1 Introduction 1735.2 Substrate Materials Developed for Immobilization of Aptamers 1755.3 Utilization of Aptamers for Sample Preparation in SPE 1775.3.1 Aptamers Utilized in Affinity Column for SPE 1815.3.2 Aptamers Utilized in Other SPE 1825.4 Aptamers Utilized in SPME 1825.4.1 Aptamers Utilized in Fiber SPME 1835.4.2 Aptamers Utilized in SBSE 1845.4.3 Aptamers Utilized in Other Formats of SPME 1855.5 Aptamers Utilized in Other Affinity Chromatography 1855.6 Aptamers Utilized in Microfluidic Separation System 1875.7 Aptamers Utilized in Magnetic Separation System 1895.7.1 Aptamers Utilized in Magnetic Solid-Phase Extraction (MSPE) 1905.7.2 Aptamers Utilized in Other Magnetic Separation Formats 1905.8 Aptamers Utilized in CE 1915.9 Aptamers Utilized in Other Sample SeparationMethods 1925.10 Conclusion and Outlook 192References 1926 Development of Aptamer-Based Colorimetric Analytical Methods 205Subash C.B. Gopinath, Thangavel Lakshmipriya,M.K.Md Arshad, and Chun Hong Voon6.1 Introduction 2056.2 Aptamer Generation for Colorimetric Assay 2066.3 Aptasensor 2066.4 Aptamer-AuNP-Based Colorimetric Assays 2076.5 Applications of AuNP-Aptamer-Based Colorimetric Assays 2116.6 Conclusions 213References 2137 Enzyme-Linked Aptamer Assay (ELAA) 219Yiyang Dong and SaiWang7.1 Introduction 2197.2 Enzyme-Linked Immunosorbent Assay 2197.3 AnalyticalMerits of Aptamer vs Antibody 2217.4 Enzyme-Linked Aptamer Assay (ELAA) 2237.5 Comparison of Direct-Competitive ELAA (dc-ELAA), Indirect-Competitive ELAA (ic-ELAA), and ELISA 2257.6 Conclusion 226References 2278 Development of Aptamer-Based Fluorescence Sensors 229SeyedM. Taghdisi, Rezvan Yazdian-Robati, Mona Alibolandi, Mohammad Ramezani, and Khalil Abnous8.1 Introduction 2298.2 Fluorescent-Dye-Based Aptasensors 2308.3 Nanoparticle-Based Aptasensors 2318.3.1 Fluorescent Aptasensors Based on Gold Nanoparticles 2318.3.2 Fluorescent Aptasensors Based on Carbon Nanomaterials 2348.3.3 Fluorescent Aptasensors Based on Silica Nanoparticles 2368.3.4 Fluorescent Aptasensors Based on Silver Nanoparticles 2388.3.5 Fluorescent Aptasensors Based on DNA Structures 2398.3.5.1 Fluorescent Aptasensors Based on DNA Nanostructures 2398.3.5.2 Fluorescent Aptasensors Based on Triple-Helix Molecular Switch (THMS) 2408.4 Conclusion 241Acknowledgment 241SuggestedWebsites 242References 2429 Development of Aptamer-Based Electrochemical Methods 247Jian-guo Xu, Li Yao, Lin Cheng, Chao Yan, andWei Chen9.1 Introduction 2479.2 Classification of Electrochemical Aptasensors 2479.3 Amperometric Aptasensors 2489.3.1 Covalent Labels 2489.3.1.1 Enzyme Labels 2489.3.1.2 Other Covalently Linked Redox Species 2509.3.2 Non-covalent Labels 2569.3.2.1 Intercalated Redox Species 2569.3.2.2 Cationic Redox Species 2609.3.3 Label-Free Aptasensors 2639.4 Potentiometric Aptasensors 2659.5 Impedimetric Aptasensors 2669.6 Electrochemiluminescence Aptasensors 2689.7 Conclusion 268References 26910 Development of Aptamer-Based Lateral Flow Assay Methods 273Miriam Jauset-Rubio, Mohammad S. El-Shahawi, Abdulaziz S. Bashammakh, Abdulrahman O. Alyoubi, and Ciara K. O'Sullivan10.1 Introduction 27310.2 Development of Aptamer-Based Lateral Flow Assay - Strategy 27510.2.1 Analogies and Differences Compared to Lateral flow Immunoassays (LFIAs) 27510.2.2 Fundamental Assay Considerations 27610.2.3 Fundamental Analytical Considerations 27710.3 Lateral Flow Aptamer Assays 27810.3.1 Sandwich Assay 27810.3.2 Competitive Assay 28110.3.3 Signal Amplification 28310.4 Summary and Perspectives 291References 29411 Development of Aptamer-Based Non-labeling Methods 301Huajie Gu, Liling Hao, and ZhoupingWang11.1 Introduction 30111.2 Surface Plasmon Resonance (SPR)-Based Aptasensor 30211.2.1 Introduction 30211.2.2 The Principle of SPR Technique 30211.2.3 The Classification of SPR Biosensors 30311.2.3.1 SPR Biosensors Based on Angular Modulation 30311.2.3.2 SPR Biosensors Based onWavelength Modulation 30411.2.3.3 SPR Biosensors Based on Amplitude Modulation 30411.2.3.4 SPR Biosensors Based on Phase Modulation 30411.2.4 The Application of Aptamer-Based SPR Technique 30411.2.4.1 Determination of the Affinity of Aptamers 30511.2.4.2 Detection Analyte Concentrations 30511.2.5 Summary and Prospects of SPR Aptasensors 31011.3 Quartz Crystal Microbalance (QCM)-Based Aptasensor 31111.3.1 Introduction 31111.3.2 The Principle of QCM Technique 31111.3.3 The Application of Aptamer-Based QCM Technique 31211.3.3.1 Determination of the Affinity of Aptamers 31211.3.3.2 Detection of Analyte Concentrations 31311.3.4 Summary and Prospect of QCM Aptasensors 31811.4 Isothermal Titration Calorimetry (ITC) 31911.4.1 Introduction 31911.4.2 The Principle of ITC Technique 31911.4.3 Thermodynamic Parameters Obtained from ITC Experiment 32011.4.4 Application of ITC in Association Between Aptamer and Target 32211.4.4.1 Interaction Between the Aptamer Domain of the Purine Riboswitch and Ligands 32211.4.4.2 Interaction Between the Cocaine-Binding Aptamer and Quinine 32411.4.4.3 Affinity Test by ITC After Systemic Evolution of Ligands by EXponential Enrichment (SELEX) 32711.4.5 Summary 32911.5 MicroScaleThermophoresis (MST) 32911.5.1 Introduction 32911.5.2 The Principle of MST Technique 33011.5.3 Application of MST in Association Between Aptamer and Target 33211.5.3.1 Interaction Between Steroid Hormones and Aptamers 33211.5.3.2 Affinity Test by MST After Systemic Evolution of Ligands by EXponential Enrichment (SELEX) 33311.5.4 Summary 335References 33512 Challenges of SELEX and Demerits of Aptamer-Based Methods 345Haiyun Liu and Jinghua Yu12.1 Introduction 34512.2 Challenges of SELEX 34712.2.1 Aptamer Degradation 34712.2.2 Purification 34812.2.3 Binding Affinity (Kd) 34812.2.4 Target Immobilization 34912.2.5 Cross-Reactivity 35012.2.6 Time and Cost 35012.2.7 Interaction of Aptamers with Intracellular Targets 35112.2.8 Bioinformatics Tools 35212.3 Demerits of Aptamer-Based Methods 35212.3.1 Sensitivity 35212.3.2 Selectivity and Specificity 35412.3.3 Reproducibility 35512.3.4 Calibration and Uncertainty 35512.3.5 Regeneration 35512.3.6 Immobilization of Aptamers 35612.4 Summary and Perspectives 356References 35713 State of the Art and Emerging Applications 365Lin-Chi Chen, Jui-HongWeng, and Pei-Wei Lee13.1 Introduction 36513.2 Frontiers of Analytical Aptamer Selection and Probe Design 36813.2.1 Biochip-Based Aptamer Selection 36813.2.2 SELEX with Next-Generation Sequencing (NGS) 37213.2.3 Aptamer Optimization and Specialized Selection 37313.2.4 In Silico Aptamer Design 37613.3 Novel Aptasensing Platforms - From Assays and Sensors to Instrumental Analyses 37813.3.1 Aptamer Assays 37813.3.2 Aptasensors 38013.3.3 Aptamer Chips 38213.3.4 Cell-Based Aptasensing 38413.4 Emerging Applications of Aptamer Diagnostics 38513.4.1 Human Disease Diagnosis 38613.4.2 Food/EnvironmentalMonitoring - Mycotoxins, Pesticides, Heavy Metal Ions 38713.4.3 Therapeutic Drug Assessment - Organ-on-a-Chip 38713.4.4 New Molecular Biology Applications - CRISPR/Cas9, Stem Cells, IHC 38813.5 Concluding Remarks - Frontiers of Frontiers 389Acknowledgments 389References 390Index 397
Responsibility: edited by Yiyang Dong.

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<http://experiment.worldcat.org/entity/work/data/5215094276#Topic/analytische_chemie> # Analytische Chemie
    a schema:Intangible ;
    schema:name "Analytische Chemie" ;
    .

<http://experiment.worldcat.org/entity/work/data/5215094276#Topic/chemistry_analytic> # Chemistry, Analytic
    a schema:Intangible ;
    schema:name "Chemistry, Analytic" ;
    .

<http://experiment.worldcat.org/entity/work/data/5215094276#Topic/science_life_sciences_biochemistry> # SCIENCE--Life Sciences--Biochemistry
    a schema:Intangible ;
    schema:name "SCIENCE--Life Sciences--Biochemistry" ;
    .

<http://worldcat.org/isbn/9783527342679>
    a schema:ProductModel ;
    schema:isbn "3527342672" ;
    schema:isbn "9783527342679" ;
    .

<http://www.worldcat.org/title/-/oclc/1085552662>
    a genont:InformationResource, genont:ContentTypeGenericResource ;
    schema:about <http://www.worldcat.org/oclc/1085552662> ; # Aptamers for analytical applications : affinity acquisition and method design
    schema:dateModified "2019-10-24" ;
    void:inDataset <http://purl.oclc.org/dataset/WorldCat> ;
    .


Content-negotiable representations

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