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Genre/Form: | Thèses et écrits académiques |
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Material Type: | Document, Thesis/dissertation, Internet resource |
Document Type: | Internet Resource, Computer File |
All Authors / Contributors: |
Mathieu Dupuy; Yann Héchard; Florence Berne; Jean-Marc Berjeaud; Laurence Mathieu; Vincent Thomas; Patrick Leprat; Université de Poitiers.; École doctorale Sciences pour l'environnement - Gay Lussac (La Rochelle / 2009-2018).; Ecologie et biologie des interactions - EBI (Poitiers).; Université de Poitiers. UFR des sciences fondamentales et appliquées. |
OCLC Number: | 892850923 |
Notes: | Titre provenant de l'écran-titre. |
Description: | 1 online resource |
Responsibility: | Mathieu Dupuy ; sous la direction de Yann Héchard et de Florence Berne. |
Abstract:
Legionella is a pathogenic gram negative bacterium, ubiquitously found in waterborne environment. The use of surface water, in open recirculating cooling circuits of nuclear power plants, causes a warming of these waters, which may cause proliferation of Legionella. To limit the development of this bacterium, under the legal thresholds, EDF implements a monochloramine treatment. The aim of our study was to improve the knowledge on the association between Legionella and their main host : amoebae, and on the factors that may influence their susceptibility to biocide treatment. We first showed the permissiveness of 12 strains of amoebae to 2 strains of L. pneumophila. Temperature is appeared as the most influential parameter, the proliferation of Legionella seems favored at 40°C. Then, we searched to develop a protocol to quantify Legionella according to its state of association. This protocol is not operational, yet. Finally, we compared the sensitivity of Legionella, under different physiological states and studied the impact of water quality and suspended matter. Post-amoeba cells and at a lesser extent, stationary cells, are the less sensitive. The effect of the matrix was demonstrated for the Vienne river water, on the other side the elimination or enrichment in suspended matter has not modified the sensitivity. This study provides a basis for a better knowledge to manage monochloramine treatment against Legionella, and for operating cooling systems.
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