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Avian and reptilian developmental biology : methods and protocols

Author: Guojun Sheng
Publisher: New York : Humana Press : Springer, [2017] ©2017
Series: Methods in molecular biology (Clifton, N.J.), v. 1650.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This volume discusses recent advances in avian and reptilian biology that have caused this diverse field to re-emerge. The chapters in this book are divided into 4 parts: genomics and transcriptomics, genetic manipulation, stem cells, and new model systems. Part I details how to perform genomic and transcriptomic analyses in birds and reptiles; Part II highlights technological advancements in avian genetic  Read more...
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Genre/Form: Laboratory manuals
Electronic books
Laboratory Manuals
Additional Physical Format: Print version:
Avian and reptilian developmental biology.
New York : Humana Press, ©2017
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Guojun Sheng
ISBN: 9781493972166 1493972162
OCLC Number: 1001282382
Description: 1 online resource (xii, 365 pages) : illustrations (some color).
Contents: Some thoughts on experimental design / Claudio D. Stern --
Comparative genomics as a foundation for evo-devo studies in birds / Phil Grayson, Simon Y.W. Sin, Timothy B. Sackton, and Scott V. Edwards --
Step-by-step guide to assemble a reptilian genome / Asier Ullate-Agote, Yingguang Frank Chan, and Athanasia C. Tzika --
Genomic and transcriptomic analyses of avian sex chromosomes and sex-linked genes / Jilin Zhang, Jing Li, and Qi Zhou --
Systems biology analyses in chicken : workflow for transcriptome and ChIP-seq analyses using the chicken skin paradigm / Yung-Chih Lai, Randall B. Widelitz, and Cheng-Ming Chuong --
Application of a CAGE method to an avian development study / Ruslan Deviatiiarov, Marina Lizio, and Oleg Gusev --
CRISPR/Cas9 in the chicken embryo / Valerie Morin, Nadege Veron, and Christophe Marcelle --
Fluorescent quail : a transgenic model system for the dynamic study of avian development / David Huss and Rusty Lansford --
Lentiviral-mediated transgenesis in songbirds / Wan-chun Liu, Marian Hruska-Plochan, and Atsushi Miyanohara --
In ovo electroporation methods in chick embryos / Hidekiyo Harada, Minoru Omi, and Harukazu Nakamura --
Genetic manipulation of the avian urogenital system using in ovo electroporation / Claire E. Hirst, Olivier Serralbo, Katie L. Ayers, Kelly N. Roeszler, and Craig A. Smith --
Enhancer analyses using chicken embryo electroporation / Masanori Uchikawa, Naoko Nishimura, Makiko Iwafuchi-Doi, and Hisato Kondoh --
Transgene introduction into the chick limb bud by electroporation / Shogo Ueda, Takayuki Suzuki, and Mikiko Tanaka --
Chicken induced pluripotent stem cells : establishment and characterization / Aurelie Fuet and Bertrand Pain --
Isolation and characterization of chicken primordial germ cells and their application in transgenesis / Jae Yong Han and Bo Ram Lee --
Handling of gametes for in vitro insemination in birds / Shusei Mizushima, Mei Matsuzaki, and Tomohiro Sasanami --
In vitro and ex ovo culture of reptilian and avian neural progenitor cells / Wataru Yamashita, Toyo Shimizu, and Tadashi Nomura --
Lifting the veil on reptile embryology : the veiled chameleon (Chamaeleo calyptratus) as a model system to study reptilian development / Raul E. Diaz Jr., Federica Bertocchini, and Paul A. Trainor --
Model clades versus model species : anolis lizards as an integrative model of anatomical evolution / Thomas J. Sanger and Bonnie K. Kircher --
Feather model for chemo- and radiation therapy-induced tissue damage / Zhicao Yue and Benhua Xu --
Early chick embryo culture device for extended continuous observation / Hans-Georg Sydow, Tobias Pieper, Christoph Viebahn, and Nikoloz Tsikolia --
Sensitive and versatile in situ hybridization protocol for gene expression analysis in developing amniote brains / Pei-Shan Hou, Takuma Kumamoto, and Carina Hanashima --
Somitogenesis and axial development in reptiles / Cindy Xu, Mariana B. Grizante, and Kenro Kusumi --
MicroCT imaging on living alligator teeth reveals natural tooth cycling / Randall B. Widelitz, Alaa Abdelhamid, M. Khalil Khan, Amr Elkarargy, Cheng-Ming Chuong, and Ping Wu.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1650.
Responsibility: edited by Guojun Sheng.

Abstract:

This volume discusses recent advances in avian and reptilian biology that have caused this diverse field to re-emerge. The chapters in this book are divided into 4 parts: genomics and transcriptomics, genetic manipulation, stem cells, and new model systems. Part I details how to perform genomic and transcriptomic analyses in birds and reptiles; Part II highlights technological advancements in avian genetic manipulation; Part III focuses on methods to handle pluripotent cells; and part IV looks at the emerging models in avian and reptilian developmental biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Avian and Reptilian Developmental Biology: Methods and Protocols explores a variety of approaches and different sauropsid models that will help facilitate communication and collaboration among researchers, which in turn will progress this field forward.

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