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Avian and Reptilian Developmental Biology : Methods and Protocols

Author: Guojun Sheng
Publisher: New York, NY Springer New York 2017
Series: Methods in Molecular Biology, 1650
Edition/Format:   Computer file : EnglishView all editions and formats
Summary:
This volume discusses recent advances in avian and reptilian biology that have caused this diverse field to re-emerge. The chapters in this book are divided into 4 parts: genomics and transcriptomics, genetic manipulation, stem cells, and new model systems. Part I details how to perform genomic and transcriptomic analyses in birds and reptiles; Part II highlights technological advancements in avian genetic  Read more...
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Additional Physical Format: Erscheint auch als:
Druck-Ausgabe
Material Type: Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Guojun Sheng
ISBN: 9781493972166 1493972162
OCLC Number: 1036305131
Description: 1 Online-Ressource (XII, 365 p. 91 illus., 75 illus. in color)
Contents: Preface... Table of Contents...Contributing Authors... Commentary1 Some Thoughts on Experimental DesignClaudio D. Stern Part I Genomics and Transcriptomics2 Comparative Genomics as a Foundation for Evo-Devo Studies in BirdsPhil Grayson, Simon Y.W. Sin, Timothy B. Sackton, and Scott V. Edwards3 A Step-by-Step Guide to Assemble a Reptilian GenomeAsier Ullate-Agote, Yingguang Frank Chan, Michel C. Milinkovitch, and Athanasia C. Tzika4 Genomic and Transcriptomic Analyses of Avian Sex Chromosomes and Sex-Linked GenesJilin Zhang, Jing Li, and Qi Zhou5 Systems Biology Analyses in Chicken: Workflow for Transcriptome and ChIP-Seq Analyses using the Chicken Skin ParadigmYung-Chih Lai, Randall B. Widelitz and, and Cheng-Ming Chuong6 Application of a CAGE Method to an Avian Development StudyRuslan Deviatiiarov, Marina Lizio, and Oleg Gusev Part II Genetic Manipulation7 CRISPR/Cas9 in the Chicken EmbryoValerie Morin, Nadege Veron, and Christophe Marcelle 8 Fluorescent Quail: A Transgenic Model System for the Dynamic Study of Avian DevelopmentDavid Huss and Rusty Lansford9 Lentiviral-Mediated Transgenesis in SongbirdsWan-chun Liu, Marian Hruska-Plochan, and Atsushi Miyanohara10 In Ovo Electroporation Methods in Chick EmbryosHidekiyo Harada, Minoru Omi, and Harukazu Nakamura11 Genetic Manipulation of the Avian Urogenital System using In Ovo ElectroporationClaire E. Hirst, Olivier Serralbo, Katie L. Ayers, Kelly N. Roeszler and Craig A. Smith12 Enhancer Analyses using Chicken Embryo ElectroporationMasanori Uchikawa, Naoko Nishimura, Makiko Iwafuchi-Doi, and Hisato Kondoh13 Transgene Introduction into the Chick Limb Bud by ElectroporationShogo Ueda, Takayuki Suzuki, and Mikiko Tanaka Part III Stem Cells14 Chicken Induced Pluripotent Stem Cells: Establishment and CharacterizationAurelie Fuet and Bertrand Pain15 Isolation and Characterization of Chicken Primordial Germ Cells and their Application in TransgenesisJae Yong Han and Bo Ram Lee16 Handling of Gametes for In Vitro Insemination in BirdsShusei Mizushima, Mei Matsuzaki, and Tomohiro Sasanami17 In Vitro and Ex Ovo Culture of Reptilian and Avian Neural Progenitor CellsWataru Yamashita, Toyo Shimizu and Tadashi Nomura Part IV New Model Systems18 Lifting the Veil on Reptile Embryology: The Veiled Chameleon (Chamaeleo calyptratus) as a Model System to Study Reptilian DevelopmentRaul E. Diaz, Jr., Federica Bertocchini, and Paul A. Trainor19 Model Clades versus Model Species: Anolis Lizards as an Integrative Model of Anatomical EvolutionThomas J. Sanger and Bonnie K. Kircher20 The Feather Model for Chemo- and Radiation Therapy-Induced Tissue DamageZhicao Yue and Benhua Xu21 An Early Chick Embryo Culture Device for Extended Continuous ObservationHans-Georg Sydow, Tobias Pieper, Christoph Viebahn, and Nikoloz Tsikolia22 A Sensitive and Versatile In Situ Hybridization Protocol for Gene Expression Analysis in Developing Amniote BrainsPei-Shan Hou, Takuma Kumamoto, and Carina Hanashima23 Somitogenesis and Axial Development in ReptilesCindy Xu, Mariana B. Grizante, and Kenro Kusumi24 Micro-CT Imaging on Living Alligator Teeth Reveals Natural Tooth CyclingRandall B. Widelitz, Alaa Abdelhamid, M. Khalil Khan, A. Elkarargy, Cheng-Ming Chuong , and Ping Wu
Series Title: Methods in Molecular Biology, 1650
Responsibility: edited by Guojun Sheng.

Abstract:

This volume discusses recent advances in avian and reptilian biology that have caused this diverse field to re-emerge. The chapters in this book are divided into 4 parts: genomics and transcriptomics, genetic manipulation, stem cells, and new model systems. Part I details how to perform genomic and transcriptomic analyses in birds and reptiles; Part II highlights technological advancements in avian genetic manipulation; Part III focuses on methods to handle pluripotent cells; and part IV looks at the emerging models in avian and reptilian developmental biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Avian and Reptilian Developmental Biology: Methods and Protocols explores a var iety of approaches and different sauropsid models that will help facilitate communication and collaboration among researchers, which in turn will progress this field forward.

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