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Basic methods in molecular biology

Author: Leonard G Davis; W Michael Kuehl; James F Battey
Publisher: Norwalk, Conn. : Appleton & Lange, ©1994.
Edition/Format:   Print book : Document   Computer File : English : 2nd edView all editions and formats
Summary:

This edition emphasizes the decisions that need to be made to select one procedure over another in the everyday workings of a molecular biology laboratory. Trouble-shooting problems that may emerge  Read more...

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Additional Physical Format: Online version:
Davis, Leonard G.
Basic methods in molecular biology.
Norwalk, Conn. : Appleton & Lange, ©1994
(OCoLC)666134767
Material Type: Document, Internet resource
Document Type: Book, Computer File, Internet Resource
All Authors / Contributors: Leonard G Davis; W Michael Kuehl; James F Battey
ISBN: 0838506429 9780838506424
OCLC Number: 29518617
Description: xiv, 777 pages : illustrations ; 26 cm
Contents: General methods --
Bacterial strains and cloning vectors --
Enzymes that modify DNA and RNA --
In vitro amplification of DNA using the polymerase chain reaction (PCR) and the thermostable Taq DNA polymerase --
DNA restriction fragment analysis and preparation --
In vitro labeling of probes and filter hybridization --
Plasmid DNA preparation from E coli hosts --
Preparation of DNA from lambda bacteriophage clones --
Subcloning fragments into plasmid vectors and plasmid construction --
Preparation of genomic DNA --
Preparation and analysis of RNA from eukaryotic cells --
Genomic cloning --
Preparation and analysis of cDNA libraries --
Preparation of subtractive cDNA --
Chain termination sequencing --
Transfection of mammalian cells --
Basic methods of protein analysis --
In situ hybridization --
Transgenic mouse preparation --
Detection and in vitro generation of specific mutations in genes and cDNAs --
Preparation of stock solutions --
Generally useful information --
Suppliers of reagents and equipment.
Responsibility: Leonard G. Davis, W. Michael Kuehl, James F. Battey.
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