skip to content
Biochemistry Laboratory Manual For Undergraduates : an Inquiry-Based Approach Preview this item
ClosePreview this item
Checking...

Biochemistry Laboratory Manual For Undergraduates : an Inquiry-Based Approach

Author: Timea Gerczei Fernandez; Scott Pattison
Publisher: Warschau/Berlin : De Gruyter Open, [2015] ©2015
Edition/Format:   eBook : Document : Government publication : EnglishView all editions and formats
Summary:
Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each  Read more...
Rating:

(not yet rated) 0 with reviews - Be the first.

Subjects
More like this

Find a copy online

Links to this item

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Material Type: Document, Government publication, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Timea Gerczei Fernandez; Scott Pattison
ISBN: 9783110411331 3110411334
OCLC Number: 1013967206
Language Note: In English.
Description: 1 online resource (186 pages) : illustrations
Contents: Frontmatter --
Preface --
Contents --
1 Introducing the Bacterial Antibiotic Sensor Mini Project --
2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function --
3 Designing Primers for Site-Directed Mutagenesis --
4 Performing Site-Directed Mutagenesis --
5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy --
6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease --
7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro --
8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry --
9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching --
10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy --
11 Understanding the Importance of Buffers in Biological Systems --
12 Molecular Visualization of an Enzyme, Acetylcholinesterase --
13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment --
14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC --
List of Figures.
Responsibility: Timea Gerczei Fernandez, Scott Pattison.
More information:

Abstract:

Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.

Reviews

User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.

Similar Items

Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Linked Data


Primary Entity

<http://www.worldcat.org/oclc/1013967206> # Biochemistry Laboratory Manual For Undergraduates : an Inquiry-Based Approach
    a schema:Book, schema:CreativeWork, schema:MediaObject ;
    library:oclcnum "1013967206" ;
    library:placeOfPublication <http://id.loc.gov/vocabulary/countries/gw> ;
    schema:about <http://experiment.worldcat.org/entity/work/data/2206780237#Topic/biowissenschaften_biologie> ; # Biowissenschaften, Biologie
    schema:about <http://experiment.worldcat.org/entity/work/data/2206780237#Topic/biochemie> ; # Biochemie
    schema:about <http://dewey.info/class/572.078/> ;
    schema:about <http://experiment.worldcat.org/entity/work/data/2206780237#Topic/labortechnik> ; # Labortechnik
    schema:author <http://experiment.worldcat.org/entity/work/data/2206780237#Person/pattison_scott> ; # Scott Pattison
    schema:author <http://experiment.worldcat.org/entity/work/data/2206780237#Person/gerczei_fernandez_timea> ; # Timea Gerczei Fernandez
    schema:bookFormat schema:EBook ;
    schema:datePublished "2015" ;
    schema:description "Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic."@en ;
    schema:exampleOfWork <http://worldcat.org/entity/work/id/2206780237> ;
    schema:genre "Government publication"@en ;
    schema:inLanguage "en" ;
    schema:isSimilarTo <http://worldcat.org/entity/work/data/2206780237#CreativeWork/> ;
    schema:name "Biochemistry Laboratory Manual For Undergraduates : an Inquiry-Based Approach"@en ;
    schema:productID "1013967206" ;
    schema:url <https://doi.org/10.2478/9783110411331> ;
    schema:workExample <http://dx.doi.org/10.2478/9783110411331> ;
    schema:workExample <http://worldcat.org/isbn/9783110411331> ;
    wdrs:describedby <http://www.worldcat.org/title/-/oclc/1013967206> ;
    .


Related Entities

<http://experiment.worldcat.org/entity/work/data/2206780237#Person/gerczei_fernandez_timea> # Timea Gerczei Fernandez
    a schema:Person ;
    schema:familyName "Gerczei Fernandez" ;
    schema:givenName "Timea" ;
    schema:name "Timea Gerczei Fernandez" ;
    .

<http://experiment.worldcat.org/entity/work/data/2206780237#Person/pattison_scott> # Scott Pattison
    a schema:Person ;
    schema:familyName "Pattison" ;
    schema:givenName "Scott" ;
    schema:name "Scott Pattison" ;
    .

<http://experiment.worldcat.org/entity/work/data/2206780237#Topic/biowissenschaften_biologie> # Biowissenschaften, Biologie
    a schema:Intangible ;
    schema:name "Biowissenschaften, Biologie"@en ;
    .

<http://worldcat.org/entity/work/data/2206780237#CreativeWork/>
    a schema:CreativeWork ;
    schema:isSimilarTo <http://www.worldcat.org/oclc/1013967206> ; # Biochemistry Laboratory Manual For Undergraduates : an Inquiry-Based Approach
    .

<http://worldcat.org/isbn/9783110411331>
    a schema:ProductModel ;
    schema:isbn "3110411334" ;
    schema:isbn "9783110411331" ;
    .

<http://www.worldcat.org/title/-/oclc/1013967206>
    a genont:InformationResource, genont:ContentTypeGenericResource ;
    schema:about <http://www.worldcat.org/oclc/1013967206> ; # Biochemistry Laboratory Manual For Undergraduates : an Inquiry-Based Approach
    schema:dateModified "2019-07-30" ;
    void:inDataset <http://purl.oclc.org/dataset/WorldCat> ;
    .

<https://doi.org/10.2478/9783110411331>
    rdfs:comment "Open Access" ;
    .


Content-negotiable representations

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.