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Brain tumor stem cells : methods and protocols

Author: Sheila K Singh; Chitra Venugopal
Publisher: New York, NY : Humana Press ; Springer, [2018] ©2019
Series: Methods in molecular biology (Clifton, N.J.), v. 1869.
Edition/Format:   eBook : Document : EnglishView all editions and formats

This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer  Read more...


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Genre/Form: Electronic books
Laboratory Manual
Laboratory manuals
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Sheila K Singh; Chitra Venugopal
ISBN: 9781493988051 1493988050
OCLC Number: 1056959671
Description: 1 online resource (xii, 254 pages) : illustrations (some color)
Contents: Introduction to brain tumor stem cells / Nicolas Yelle, David Bakhshinyan, Chitra Venugopal, and Sheila K. Singh --
Isolation and culture of glioblastoma brain tumor stem cells / Charles Chesnelong, Ian Restall, and Samuel Weiss --
Establishment and culture of patient-derived primary medulloblastoma cell lines / Sara Badodi, Silvia Marino, and Loredana Guglielmi --
Bioinformatic strategies for the genomic and epigenomic characterization of brain tumors / Vijay Ramaswamy and Michael D. Taylor --
Detecting stem cell marker expression using the NanoString nCounter system / Scott Ryall, Anthony Arnoldo, Javal Sheth, Sheila K. Singh, and Cynthia Hawkins --
Flow cytometric analysis of brain tumor stem cells / Minomi K. Subapanditha, Ashley A. Adile, Chitra Venugopal, and Sheila K. Singh --
In vitro self-renewal assays for brain tumor stem cells / Mathieu Seyfrid, David Bobrowski, David Bakhshinyan, Nazanin Tatari, Chitra Venugopal, and Sheila K. Singh --
Differentiation of brain tumor initiating cells / Michelle M. Kameda-Smith, Minomi K. Subapanditha, Sabra K. Salim, Chitra Venugopal, and Sheila K. Singh --
Study of brain tumor stem cell migration / Montserrat Lara-Velazquez, Rawan Al-kharboosh, Luis Prieto, Paula Schiapparelli, and Alfredo Quinones-Hinojosa --
Study of brain tumor stem cell invasion / Rawan Al-kharboosh, Montserrat Lara-Velazquez, Luis Prieto, Rachel Sarabia-Estrada, and Alfredo Quinones-Hinojosa --
Cell cycle dynamics in glioma cancer stem cells / Ingrid Qemo and Lisa A. Porter --
Embryonic stem cell models of human brain tumors / Ludivine Coudiere Morrison, Nazanin Tatari, and Tamra E. Werbowetski-Ogilvie --
Chromatin immunoprecipitation (ChIP) protocols for the cancer and developmental biology laboratory / Hunter McColl, Jamie L. Zagozewski, and David D. Eisenstat --
EPH profiling of BTIC populations in glioblastoma multiforme using CyTOF / Amy X. Hu, Jarrett J. Adams, Parvez Vora, Maleeha Qazi, Sheila K. Singh, Jason Moffat, and Sachdev S. Sidhu --
Pooled lentiviral CRISPR-Cas9 screens for functional genomics in mammalian cells / Michael Aregger, Megha Chandrashekhar, Amy Hin Yan Tong, Katherine Chan, and Jason Moffat --
In vitro assays for screening small molecules / Ashley A. Adile, David Bakhshinyan, Chitra Venugopal, and Sheila K. Singh --
Drug delivery in an orthotopic tumor stem cell-based model of human glioblastoma / Elena Binda, Alberto Visioli, Nadia Trivieri, and Angelo Luigi Vescovi --
Engineering inducible knock-in mice to model oncogenic brain tumor mutations from endogenous loci / Jon D. Larson and Suzanne J. Baker --
In vivo murine models of brain metastasis / Mohini Singh, Neil Savage, and Sheila K. Singh --
Cellular magnetic resonance imaging for tracking metastatic cancer cells in the brain / Katie M. Parkins, Ashley V. Makela, Amanda M. Hamilton, and Paula J. Foster.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1869.
Responsibility: edited by Sheila K. Singh, Chitra Venugopal.


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