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Brain tumor stem cells : methods and protocols

Author: Sheila K Singh; Chitra Venugopal
Publisher: New York, NY : Humana Press ; Springer, [2018] ©2019
Series: Methods in molecular biology (Clifton, N.J.), v. 1869.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer  Read more...

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Genre/Form: Electronic books
Laboratory Manual
Laboratory manuals
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Sheila K Singh; Chitra Venugopal
ISBN: 9781493988051 1493988050
OCLC Number: 1056959671
Description: 1 online resource (xii, 254 pages) : illustrations (some color)
Contents: Introduction to brain tumor stem cells / Nicolas Yelle, David Bakhshinyan, Chitra Venugopal, and Sheila K. Singh --
Isolation and culture of glioblastoma brain tumor stem cells / Charles Chesnelong, Ian Restall, and Samuel Weiss --
Establishment and culture of patient-derived primary medulloblastoma cell lines / Sara Badodi, Silvia Marino, and Loredana Guglielmi --
Bioinformatic strategies for the genomic and epigenomic characterization of brain tumors / Vijay Ramaswamy and Michael D. Taylor --
Detecting stem cell marker expression using the NanoString nCounter system / Scott Ryall, Anthony Arnoldo, Javal Sheth, Sheila K. Singh, and Cynthia Hawkins --
Flow cytometric analysis of brain tumor stem cells / Minomi K. Subapanditha, Ashley A. Adile, Chitra Venugopal, and Sheila K. Singh --
In vitro self-renewal assays for brain tumor stem cells / Mathieu Seyfrid, David Bobrowski, David Bakhshinyan, Nazanin Tatari, Chitra Venugopal, and Sheila K. Singh --
Differentiation of brain tumor initiating cells / Michelle M. Kameda-Smith, Minomi K. Subapanditha, Sabra K. Salim, Chitra Venugopal, and Sheila K. Singh --
Study of brain tumor stem cell migration / Montserrat Lara-Velazquez, Rawan Al-kharboosh, Luis Prieto, Paula Schiapparelli, and Alfredo Quinones-Hinojosa --
Study of brain tumor stem cell invasion / Rawan Al-kharboosh, Montserrat Lara-Velazquez, Luis Prieto, Rachel Sarabia-Estrada, and Alfredo Quinones-Hinojosa --
Cell cycle dynamics in glioma cancer stem cells / Ingrid Qemo and Lisa A. Porter --
Embryonic stem cell models of human brain tumors / Ludivine Coudiere Morrison, Nazanin Tatari, and Tamra E. Werbowetski-Ogilvie --
Chromatin immunoprecipitation (ChIP) protocols for the cancer and developmental biology laboratory / Hunter McColl, Jamie L. Zagozewski, and David D. Eisenstat --
EPH profiling of BTIC populations in glioblastoma multiforme using CyTOF / Amy X. Hu, Jarrett J. Adams, Parvez Vora, Maleeha Qazi, Sheila K. Singh, Jason Moffat, and Sachdev S. Sidhu --
Pooled lentiviral CRISPR-Cas9 screens for functional genomics in mammalian cells / Michael Aregger, Megha Chandrashekhar, Amy Hin Yan Tong, Katherine Chan, and Jason Moffat --
In vitro assays for screening small molecules / Ashley A. Adile, David Bakhshinyan, Chitra Venugopal, and Sheila K. Singh --
Drug delivery in an orthotopic tumor stem cell-based model of human glioblastoma / Elena Binda, Alberto Visioli, Nadia Trivieri, and Angelo Luigi Vescovi --
Engineering inducible knock-in mice to model oncogenic brain tumor mutations from endogenous loci / Jon D. Larson and Suzanne J. Baker --
In vivo murine models of brain metastasis / Mohini Singh, Neil Savage, and Sheila K. Singh --
Cellular magnetic resonance imaging for tracking metastatic cancer cells in the brain / Katie M. Parkins, Ashley V. Makela, Amanda M. Hamilton, and Paula J. Foster.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1869.
Responsibility: edited by Sheila K. Singh, Chitra Venugopal.

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