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Branching and intercellular communication in the Section V cyanobacterium <i>M</i><i>astigocladus laminosus</i>, a complex multicellular prokaryote
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Branching and intercellular communication in the Section V cyanobacterium Mastigocladus laminosus, a complex multicellular prokaryote

Author: Dennis J Nürnberg Affiliation: School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London, UKVicente Mariscal Affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, Américo Vespucio 49, Seville, SpainJamie Parker Affiliation: School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London, UKGiulia Mastroianni Affiliation: School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London, UKEnrique Flores Affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, Américo Vespucio 49, Seville, SpainAll authors
Edition/Format: Article Article : English
Publication:Molecular Microbiology, v91 n5 (March 2014): 935-949
Summary:
The filamentous Section V cyanobacterium Mastigocladus laminosus is one of the most morphologically complex prokaryotes. It exhibits cellular division in multiple planes, resulting in the formation of true branches, and cell differentiation into heterocysts, hormogonia and necridia. Here, we investigate branch formation and intercellular communication in M. laminosus. Monitoring of membrane rearrangement suggests that branch formation results from a randomized direction of cell growth. Transmission electron microscopy reveals cell junction structures likely to be involved in intercellular communication. We identify a sepJ gene, coding for a potential key protein in intercellular communication, and show that SepJ is localized at the septa. To directly investigate intercellular communication, we loaded the fluorescent tracer 5-carboxyfluorescein diacetate into the cytoplasm, and quantified its intercellular exchange by fluorescence recovery after photobleaching. Results demonstrate connectivity of the main trichome and branches, enabling molecular exchange throughout the filament network. Necridia formation inhibits further molecular exchange, determining the fate of a branch likely to become a hormogonium. Cells in young, narrow trichomes and hormogonia exhibited faster exchange rates than cells in older, wider trichomes. Signal transduction to co-ordinate movement of hormogonia might be accelerated by reducing cell volume.  Read more...
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Document Type: Article
All Authors / Contributors: Dennis J Nürnberg Affiliation: School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London, UK; Vicente Mariscal Affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, Américo Vespucio 49, Seville, Spain; Jamie Parker Affiliation: School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London, UK; Giulia Mastroianni Affiliation: School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London, UK; Enrique Flores Affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, Américo Vespucio 49, Seville, Spain; Conrad W Mullineaux Affiliation: School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London, UK
ISSN:0950-382X
Language Note: English
Unique Identifier: 5539237782
Notes: For correspondence. E-mail c.mullineaux@qmul.ac.uk; Tel. (+44) 20 7882 3645; Fax (+44) 20 8983 0973.
Awards:
Other Titles: Intercellular communication in Mastigocladus laminosus
Responsibility: D. J. Nürnberg et al.

Abstract:

The filamentous Section V cyanobacterium Mastigocladus laminosus is one of the most morphologically complex prokaryotes. It exhibits cellular division in multiple planes, resulting in the formation of true branches, and cell differentiation into heterocysts, hormogonia and necridia. Here, we investigate branch formation and intercellular communication in M. laminosus. Monitoring of membrane rearrangement suggests that branch formation results from a randomized direction of cell growth. Transmission electron microscopy reveals cell junction structures likely to be involved in intercellular communication. We identify a sepJ gene, coding for a potential key protein in intercellular communication, and show that SepJ is localized at the septa. To directly investigate intercellular communication, we loaded the fluorescent tracer 5-carboxyfluorescein diacetate into the cytoplasm, and quantified its intercellular exchange by fluorescence recovery after photobleaching. Results demonstrate connectivity of the main trichome and branches, enabling molecular exchange throughout the filament network. Necridia formation inhibits further molecular exchange, determining the fate of a branch likely to become a hormogonium. Cells in young, narrow trichomes and hormogonia exhibited faster exchange rates than cells in older, wider trichomes. Signal transduction to co-ordinate movement of hormogonia might be accelerated by reducing cell volume.

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