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C-di-GMP signaling : methods and protocols

Author: Karin Sauer
Publisher: New York : Humana Press : Springer, [2017] ©2017
Series: Methods in molecular biology (Clifton, N.J.), v. 1657.
Edition/Format:   eBook : Document : EnglishView all editions and formats
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Genre/Form: Laboratory Manual
Electronic books
Laboratory manuals
Additional Physical Format: Print version:
C-di-GMP signaling.
New York : Humana Press, ©2017
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Karin Sauer
ISBN: 9781493972401 1493972405
OCLC Number: 1003291650
Description: 1 online resource (xiv, 486 pages) : illustrations (some color)
Contents: Discovery of the second messenger Cyclic di-GMP / Ute Romling and Michael Y. Galperin --
Enzymatic production of c-di-GMP using a thermophilic diguanylate cyclase / Prabhadevi Venkataramani and Zhao-Xun Liang --
Synthesis of [32P]-c-di-GMP for diguanylate cyclase and phosphodiesterase activity determinations / Barbara I. Kazmierczak --
High-performance liquid chromatography (HPLC)-based detection and quantitation of cellular c-di-GMP / Olga E. Petrova and Karin Sauer --
Identification and quantification of cyclic di-guanosine monophosphate and its linear metabolites by reversed-phase LC-MS/MS / Heike Bahre and Volkhard Kaever --
Detection of cyclic dinucleotides by STING / Xiao-Xia Du and Xiao-Dong Su --
Spectrophotometric and mass spectroscopic methods for the quantification and kinetic evaluation of in vitro c-di-GMP synthesis / Geoffrey B. Severin and Christopher M. Waters --
Gauging and visualizing c-di-GMP levels in Pseudomonas aeruginosa using fluorescence-based biosensors / Morten Rybtke, Song Lin Chua, Joey Kuok Hoong Yam, Michael Givskov, Liang Yang, and Tim Tolker-Nielsen --
Cyclic di-GMP-responsive transcriptional reporter bioassays in Pseudomonas aeruginosa / Bradley R. Borlee, Grace I. Borlee, Kevin H. Martin, and Yasuhiko Irie --
Live flow cytometry analysis of c-di-GMP levels in single cell populations / ongchan Yeo, Xin C. Wang, and Ming C. Hammond --
Experimental detection and visualization of the extracellular matrix in macrocolony biofilms / Diego O. Serra and Regine Hengge --
Congo red stain identifies matrix overproduction and is an indirect measurement for c-di-GMP in many species of bacteria / Christopher J. Jones and Daniel J. Wozniak --
Type IV Pili-dependent motility as a tool to determine the activity of c-di-GMP modulating enzymes in Myxococcus xanthus / Dorota Skotnicka and Lotte Søgaard-Andersen --
Using light-activated enzymes for modulating intracellular c-di-GMP levels in bacteria / Min-Hyung Ryu, Anastasia Fomicheva, Lindsey O'Neal, Gladys Alexandre, and Mark Gomelsky --
Analysis of c-di-GMP levels synthesized by a photoreceptor protein in response to different light qualities using an in vitro enzymatic assay / Veronika Angerer, Lars-Oliver Essen, and Annegret Wilde --
Probing the role of cyclic di-GMP signaling systems in disease using Chinese radish / Shi-Qi An, Ji-Liang Tang, and J. Maxwell Dow --
Contribution of cyclic di-GMP in the control of type III and type VI secretion in Pseudomonas aeruginosa / Ronan R. McCarthy, Martina Valentini, and Alain Filloux --
Semiquantitative analysis of the red, dry, and rough colony morphology of Salmonella enterica serovar typhimurium and Escherichia coli using congo red / Annika Cimdins and Roger Simm --
Fluorescent 2-aminopurine c-di-GMP and GpG analogs as PDE probes / Jie Zhou, Clement Opoku-Temeng, and Herman O. Sintim --
Measuring cyclic diguanylate (c-di-GMP)-specific phosphodiesterase activity Using the MANT-c-di-GMP assay / Dorit Eli, Trevor E. Randall, Henrik Almblad, Joe J. Harrison, and Ehud Banin --
Determining phosphodiesterase activity (radioactive assay) / Barbara I. Kazmierczak --
Determining diguanylate cyclase activity (radioactive assay) / Barbara I. Kazmierczak --
Detection of c-di-GMP-responsive DNA binding / Jacob R. Chambers and Karin Sauer --
Use of nonradiochemical DNAse footprinting to analyze c-di-GMP modulation of DNA-binding proteins / Claudine Baraquet and Caroline S. Harwood --
Detection of cyclic di-GMP binding proteins utilizing a biotinylated cyclic di-GMP pull-down assay / Jacob R. Chambers and Karin Sauer --
Probing protein-protein interactions with genetically encoded photoactivatable cross-linkers / Richard B. Cooley and Holger Sondermann --
Identification of c-di-AMP-binding proteins using magnetic beads / Jan Kampf, Jan Gundlach, Christina Herzberg, Katrin Treffon, and Jorg Stulke --
Pull-down with a c-di-GMP-specific capture compound coupled to mass spectrometry as a powerful tool to identify novel effector proteins / Benoıt-Joseph Laventie, Timo Glatter, and Urs Jenal --
Identification of c-di-GMP-responsive riboswitches / Johann Peltier and Olga Soutourina --
Isothermal titration calorimetry to determine apparent dissociation constants (Kd) and stoichiometry of interaction (n) of C-di-GMP binding proteins / Bruno Y. Matsuyama, Petya V. Krasteva, and Marcos V.A.S. Navarro --
Targeting c-di-GMP signaling, biofilm formation, and bacterial motility with small molecules / Clement Opoku-Temeng and Herman O. Sintim --
Discovering selective diguanylate cyclase inhibitors : from PleD to discrimination of the active site of Cyclic-di-GMP phosphodiesterases / S. Rinaldo, G. Giardina, F. Mantoni, A. Paiardini, Alessio Paone, and Francesca Cutruzzola --
High-throughput screening for compounds that modulate the cellular c-di-GMP level in bacteria / Julie Groizeleau, Jens Bo Andersen, Michael Givskov, Jens Berthelsen, and Tim Tolker-Nielsen --
Genetic tools to study c-di-GMP-dependent signaling in Pseudomonas aeruginosa / Livia Leoni, Sarika Vishnu Pawar, and Giordano Rampioni.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1657.
Responsibility: edited by Karin Sauer.

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