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Cellular Electron Microscopy

Author: J Richard McIntosh
Publisher: Amsterdam ; Boston : Elsevier/Academic Press, 2007.
Series: Methods in cell biology, v. 79.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. This book will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book's five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D,  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Cellular electron microscopy.
Amsterdam ; Boston : Elsevier/Academic Press, ©2007
(OCoLC)85847813
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: J Richard McIntosh
ISBN: 9780080475035 0080475035 0123706475 9780123706478 1281003573 9781281003577 9786611003579 6611003576
OCLC Number: 145505784
Language Note: Text in English.
Description: 1 online resource (xxvii, 850 pages, 28 unnumbered pages of plates) : illustrations
Contents: The Physics of Rapid Cooling and Its Implications for Cryoimmobilization of Cells / J. Dubochet --
Cryopreparation Methods for Electron Microscopy of Selected Model Systems / K. McDonald --
Cryopreparation Methodology for Plant Cell Biology / M.W. Hess --
Correlative Light and Electron Microscopy of Early Caenorhabditis elegans Embryos in Mitosis / T. Muller-Reichert --
Understanding Microtubule Organizing Centers by Comparing Mutant and Wild-Type Structures with Electron Tomography / E.T. O'Toole --
Whole-Cell Investigation of Microtubule Cytoskeleton Architecture by Electron Tomography / J.L. Hoog --
Electron Microscopy of Archaea / C.E. Robertson --
Reconstructing Mammalian Membrane Architecture by Large Area Cellular Tomography / B.J. Marsh --
Visualization of Membrane-Cytoskeletal Interactions During Plant Cytokinesis / M.S. Otegui --
Electron Tomographic Methods for Studying the Chemical Synapse / C.P. Arthur --
Using Electron Microscopy to Understand Functional Mechanisms of Chromosome Alignment on the Mitotic Spindle / B.F. McEwen --
Electron Microscopic Analysis of the Leading Edge in Migrating Cells / T. Svitkina --
Imaging Actomyosin In Situ / K.A. Taylor --
Electron Tomography of Bacterial Chemotaxis Receptor Assemblies / P. Zhang --
How to "Read" a Vitreous Section / J. Dubochet --
Single-Particle Electron Cryomicroscopy of the Ion Channels in the Excitation-Contraction Coupling Junction / I.I. Serysheva --
Electron Microscopy of Microtubule-Based Cytoskeletal Machinery / A. Hoenger --
Reconstructing the Endocytotic Machinery / Y. Cheng --
3D Immunolocalization with Plastic Sections / M.K. Morphew --
Electron Microscopy Analysis of Viral Morphogenesis / A. Pelchen-Matthews --
Electron Tomography of Immunolabeled Cryosections / M.S. Ladinsky --
Visualizing Macromolecules with Fluoronanogold: From Photon Microscopy to Electron Tomography / T. Cheutin --
Markers for Correlated Light and Electron Microscopy / G.E. Sosinsky --
Localizing Specific Elements Bound to Macromolecules by EFTEM / R.D. Leapman --
Localization of Protein Complexes by Pattern Recognition / C. Best --
The Application of Energy-Filtered Electron Microscopy to Tomography of Thick, Selectively Stained Biological Samples / J.C. Bouwer --
Optimization of Image Collection for Cellular Electron Microscopy / P. Mooney --
Future Directions for Camera Systems in Electron Microscopy / N. Xuong --
Structure Determination In Situ by Averaging of Tomograms / F. Forster --
Methods for Image Segmentation in Cellular Tomography / K. Sandberg --
Database Resources for Cellular Electron Microscopy / M.E. Martone.
Series Title: Methods in cell biology, v. 79.
Responsibility: edited by J. Richard McIntosh.
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Abstract:

Advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. This book  Read more...

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