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Cellular interactions of probiotic bacteria with intestinal and immune cells : cell biology research progress

Publisher: New York : Nova Biomedical, [2017]
Series: Cell biology research progress.; Bacteriology research developments series.
Edition/Format:   eBook : Document : English
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Genre/Form: Electronic books
Additional Physical Format: (OCoLC)993773950
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
ISBN: 9781536123388 1536123382
OCLC Number: 1002848043
Description: 1 online resource : illustrations
Contents: Preface; Chapter 1; Introduction; 1.1. History and Development of Probiotic Bacteria; 1.1.1. Definition of Probiotics and Probiotic-Active Substances; 1.1.2. Probiotic Properties and Classification; 1.2. Microencapsulation --
Mechanisms to Protect Sensitive Probiotic Strains; 1.2.1. Microencapsulation of Probiotic Bacteria; 1.3. Gastrointestinal (GI) Tract; 1.3.1. Functions of Microbiota in the GI Tract; 1.4. Gut-Associated Immunological Tissue (GALT); 1.4.1. Structure and Function of the GALT; 1.4.2. Probiotics and the GALT; 1.5. Immune System; 1.5.1. Innate Immunological Responses 1.5.2. Adaptive Immunological Responses1.6. Probiotic Immunomodulatory Activities; 1.6.1. Probiotic Effects on the Innate Immune System; 1.6.2. Probiotic Effects on the Adaptive Immune System; 1.7. Confocal Microscopy; 1.7.1. Fluorescent Dyes; 1.7.2. Fluorescent Proteins; 1.7.2.1. Humanized Monster Green® Fluorescent Protein (hMGFP); 1.7.2.2. DsRed2 Fluorescent Protein; 1.7.3. Intracellular Tracking of DNA, RNA and Proteins Using Image Analysis Software; 1.7.3.1. Fluorescence Correlation Spectroscopy (FCS); 1.7.3.2. Raster Image Correlation Spectroscopy (RICS); 1.8. Overall Aim of Study Chapter 2Growth of Probiotic Bacteria in Mammalian Cell Culture Conditions and Confocal Microscopy Analysis of Microencapsulated Probiotic Bacteria; 2.1. Introduction; 2.1.1. Microencapsulation of Probiotic Bacteria; 2.1.2. Probiotic Bacteria and Pathogenic Bacteria Strains; 2.2. Aims and Objectives; 2.3. Materials and Methods; 2.3.1. Bacterial Growth Curves; 2.3.1.1. Bacteria Cells and Growing Media; 2.3.1.2. Growth Curves for L. acidophilus, B. lactis and S. pyogenes; 2.3.2. Bacteria Growth Curves in Mammalian Cell Culture Conditions; 2.3.2.1. Bacteria and Mammalian Cell Culture Reagents 2.3.2.2. Bacteria Growth Curves in Mammalian Cell Culture Conditions2.3.3. Viability Standard Curve; 2.3.3.1. Reagents; 2.3.3.2. Staining of Probiotic Bacteria with LIVE/DEAD® BacLight™ Bacterial Viability and Counting Kit (Invitrogen) and Development of a Viability Standard Curve; 2.3.4. Microencapsulation of Bacteria and the Development of a Viability Standard Curve; 2.3.4.1. Microencapsulation Materials; 2.3.4.2. Microencapsulation of Probiotic Bacteria and Incubation on MRS Agar and in Mammalian Cell Culture Media; 2.3.4.2.1. MRS Agar and Standard Anaerobic Bacteria Incubation Conditions 2.3.4.2.2. Mammalian Cell Culture Media and Conditions2.3.4.3. Staining of Bacteria with LIVE/DEAD® BacLight™ Bacterial Viability and Counting Kit (Invitrogen) and Microencapsulation of Bacteria; 2.3.4.4. Laser Scanning Confocal Microscopy (LSCM) Analysis of Microencapsulated Bacteria; 2.3.4.5. Analysis of Microencapsulated Bacteria Viability Using the Bitplane Imaris Software; 2.3.5. Statistics; 2.4. Results; 2.4.1. Bacteria Growth Curves in Broth and Mammalian Cell Culture Conditions; 2.4.2. Viability Standard Curve; 2.4.3. Microencapsulation of Bacteria
Series Title: Cell biology research progress.; Bacteriology research developments series.
Responsibility: Sarah Moore and Kasipathy Kailasapathy.

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